Date published: 2025-9-6

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Cdc45 抗体 (G-12): sc-55569

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说明书
  • Cdc45 抗体 G-12 是小鼠单克隆 IgG3 κ,Cdc45抗体, 在24篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1-300,在Cdc45的human起源的N-terminus处进行映射
  • Cdc45 抗体 (G-12) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Cdc45
  • 抗Cdc45抗体(G-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cdc45 (G-12): sc-55569免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRPm-IgGκ BP-HRP是Cdc45 Antibody (G-12) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与Cdc45 Antibody (G-12) 打包提供(请参阅下面的订购信息)。

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関連項目

Cdc45 抗体(G-12)是一种小鼠单克隆 IgG3 kappa 轻链抗体,可通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附试验(ELISA)检测小鼠、大鼠和人源的 Cdc45 蛋白。抗 Cdc45 抗体 (G-12) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Cdc45 蛋白在 DNA 复制的启动过程中起着至关重要的作用,是复制前复合物的重要组成部分。Cdc45 与 Cdc6 和 MCM(迷你染色体维护)复合物等其他重要蛋白相互作用,确保 DNA 复制准确高效地进行。这一功能尤为重要,因为 DNA 复制错误会导致基因组不稳定,而这正是许多癌症的特征。通过促进 MCM 复合物装载到 DNA 上,Cdc45 可帮助调节细胞周期从 G1 期向 S 期的过渡,从而确保细胞的正常分裂和功能。Cdc45 (G-12) 抗体检测这一关键蛋白使其成为研究细胞周期调控和 DNA 复制机制的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc45 抗体 (G-12) 参考文献:

  1. Cdc7 激酶对 MCM4 的磷酸化促进了它与染色质上的 Cdc45 的相互作用。  |  Masai, H., et al. 2006. J Biol Chem. 281: 39249-61. PMID: 17046832
  2. cdc25 是一种特异性酪氨酸磷酸酶,可直接激活 p34cdc2。  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
  3. 酵母(Saccharomyces cerevisiae)cdc突变体中人工染色体的有丝分裂传递。  |  Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
  4. CDC45 的高丰度可通过提高 HSPA6 抑制细胞增殖。  |  Fu, Y., et al. 2022. Cell Prolif. 55: e13257. PMID: 35642733
  5. 将 CDC45 验证为诊断和预后胃癌的新型生物标记物。  |  Wu, L., et al. 2024. PeerJ. 12: e17130. PMID: 38515458
  6. CDC45 通过影响细胞周期促进肺腺癌的干性和转移。  |  Liu, Y., et al. 2024. J Transl Med. 22: 335. PMID: 38589907
  7. 细胞周期的新转折  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
  8. 含有 CDC27 和 CDC16 的 20S 复合物可催化泛素与细胞周期蛋白 B 的有丝分裂特异性连接。  |  King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
  9. 通过酵母互补克隆出 CDC34 细胞周期基因的人类同源物。  |  Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
  10. 哺乳动物的 p50Cdc37 是 Hsp90 的蛋白激酶靶向亚基,能与 Cdk4 结合并使其稳定。  |  Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
  11. 一种与酵母 Cdc6p 相关的人类蛋白质。  |  Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175
  12. 酿酒酵母 CDC45 的人类同源物。  |  Saha, P., et al. 1998. J Biol Chem. 273: 18205-9. PMID: 9660782

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc45 抗体 (G-12)

sc-55569
200 µg/ml
$316.00

Cdc45 (G-12): m-IgGκ BP-HRP 套装

sc-521020
200 µg Ab, 40 µg BP
$354.00

Cdc45 (G-12): m-IgG3 BP-HRP 套装

sc-550331
200 µg Ab; 40 µg BP
$354.00

Cdc45 抗体 (G-12) AC

sc-55569 AC
500 µg/ml, 25% agarose
$416.00

Cdc45 抗体 (G-12) HRP

sc-55569 HRP
200 µg/ml
$316.00

Cdc45 抗体 (G-12) FITC

sc-55569 FITC
200 µg/ml
$330.00

Cdc45 抗体 (G-12) PE

sc-55569 PE
200 µg/ml
$343.00

Cdc45 抗体 (G-12) Alexa Fluor® 488

sc-55569 AF488
200 µg/ml
$357.00

Cdc45 抗体 (G-12) Alexa Fluor® 546

sc-55569 AF546
200 µg/ml
$357.00

Cdc45 抗体 (G-12) Alexa Fluor® 594

sc-55569 AF594
200 µg/ml
$357.00

Cdc45 抗体 (G-12) Alexa Fluor® 647

sc-55569 AF647
200 µg/ml
$357.00

Cdc45 抗体 (G-12) Alexa Fluor® 680

sc-55569 AF680
200 µg/ml
$357.00

Cdc45 抗体 (G-12) Alexa Fluor® 790

sc-55569 AF790
200 µg/ml
$357.00

What is your recommended western blotting secondary antibody for use with Cdc45 (G-12): sc-55569?

Asked by: jenniferc
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-02-04
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Rated 4 out of 5 by from good antiobodyworked for WB for both whole cell lysates and chromatin samples in U2OS and 293FT cells. On chromatin we see an additional strong non-specific band just below 50 kDa.
Date published: 2022-10-23
Rated 4 out of 5 by from Worked in WBWorked fine at 1:500 in 10% milk, though you can see some non-specific bands.
Date published: 2017-08-30
Rated 1 out of 5 by from Not useful for meIt did not work in my hands. I saw a mix of bands, but none of them in the right place.
Date published: 2017-08-21
Rated 5 out of 5 by from Reliable results!Cdc45 monoclonal antibody (G-12) gives consistent and reliable results in WB and has been validated against Cdc45 transfectants, and for endogenous detection.
Date published: 2017-01-19
Rated 5 out of 5 by from Works well for IF applicationsWorks well for IF applications, positive in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Publication in WBPublication in WB, FCM and IF. Good data obtained with human osteosarcoma cell line U2OS. -SCBT Publication Review
Date published: 2015-04-02
Rated 5 out of 5 by from WB: Detects endogenous Cdc45 in HeLa andWB: Detects endogenous Cdc45 in HeLa and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-02-22
Rated 5 out of 5 by from WB: Single strong band in AWB: Single strong band in A-431 nuclear extract. -SCBT QC
Date published: 2015-01-20
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Cdc45 抗体 (G-12) is rated 4.3 out of 5 by 9.
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