Date published: 2025-9-9
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CD40 Antibody (LOB-11) - Western Blotting - Image 375144
CD40 抗体 (LOB-11): sc-13528. BJAB (A), NAMALWA (B), Raji (C) 和 GA-10 (D) 全细胞裂解液中 CD40 表达的 Western 印迹分析.
CD40 抗体 (LOB-11): sc-13528. BJAB (A), NAMALWA (B), Raji (C) 和 GA-10 (D) 全细胞裂解液中 CD40 表达的 Western 印迹分析.

CD40 抗体 (LOB-11): sc-13528

4.0(8)
寫評論提問

说明书
  • CD40抗体(LOB-11)是小鼠单克隆IgG2a κ, 在9篇文献中引用，规格为200 µg/ml
  • 针对CD40的胞外结构域，源自human
  • CD40 抗体 (LOB-11) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测human 来源的 CD40
  • 抗CD40抗体（LOB-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD40 (LOB-11): sc-13528的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 CD40 抗体 (LOB-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD40 抗体 (LOB-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD40抗体（LOB-11）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测人类CD40蛋白。CD40 (LOB-11) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。CD40 蛋白在免疫反应中起着至关重要的作用，主要通过介导 B 细胞和 T 辅助细胞之间的交流。CD40位于B细胞表面，与激活的T细胞上表达的CD40配体（CD40L）相互作用，这对B细胞的活化和分化至关重要。这种相互作用不仅促进B细胞的增殖，而且有助于免疫球蛋白类转换，使B细胞能够产生不同类型的抗体，包括IgE，这对于针对病原体产生有效的免疫反应至关重要。CD40在免疫调节中的重要性体现在它参与各种免疫过程，包括激活下游信号通路，从而产生细胞因子和其他介质，这些介质对于产生强大的免疫反应至关重要。了解CD40的功能和意义对于制定针对免疫相关疾病的治疗策略和提高疫苗功效至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD40 抗体 (LOB-11)

sc-13528
200 µg/ml
$316.00

CD40 (LOB-11): m-IgG Fc BP-HRP 套装

sc-525474
200 µg Ab; 10 µg BP
$354.00

CD40 (LOB-11): m-IgG2a BP-HRP 套装

sc-546275
200 µg Ab; 10 µg BP
$354.00

CD40 抗体 (LOB-11) AC

sc-13528 AC
500 µg/ml, 25% agarose
$416.00

CD40 抗体 (LOB-11) HRP

sc-13528 HRP
200 µg/ml
$316.00

CD40 抗体 (LOB-11) FITC

sc-13528 FITC
200 µg/ml
$330.00

CD40 抗体 (LOB-11) PE

sc-13528 PE
200 µg/ml
$343.00

CD40 抗体 (LOB-11) Alexa Fluor® 488

sc-13528 AF488
200 µg/ml
$357.00

CD40 抗体 (LOB-11) Alexa Fluor® 546

sc-13528 AF546
200 µg/ml
$357.00

CD40 抗体 (LOB-11) Alexa Fluor® 594

sc-13528 AF594
200 µg/ml
$357.00

CD40 抗体 (LOB-11) Alexa Fluor® 647

sc-13528 AF647
200 µg/ml
$357.00

CD40 抗体 (LOB-11) Alexa Fluor® 680

sc-13528 AF680
200 µg/ml
$357.00

CD40 抗体 (LOB-11) Alexa Fluor® 790

sc-13528 AF790
200 µg/ml
$357.00

Can CD40 (LOB-11): sc-13528 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD40 (LOB-11): sc-13528 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Very goodVery good antibody. It works in WB of whole cell lysates of human cells. 1/250 dilution.
Date published: 2020-06-10
Rated 1 out of 5 by from dose not workdoes not work at all on PFA-fixed paraffin-embeded human retina sections, unlike the CD40(H-10), these two are tested under same conditions.
Date published: 2018-02-15
Rated 5 out of 5 by from Publishable Western blot results using M12Publishable Western blot results using M12.4.1. -SCBT Publication Review
Date published: 2015-06-10
Rated 5 out of 5 by from Published good quality flow cytometry dataPublished good quality flow cytometry data in human PC-3M and PC-3S. -SCBT Publication Review
Date published: 2015-06-07
Rated 4 out of 5 by from Robust WB signal obtained using BJAB wholeRobust WB signal obtained using BJAB whole cell lysate. -SCBT QC
Date published: 2015-05-11
Rated 4 out of 5 by from Produced great FCM data for GAProduced great FCM data for GA-10 cells. -SCBT QC
Date published: 2015-04-21
Rated 4 out of 5 by from Robust immunoperoxidase membrane stainingRobust immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human lymphoma. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Publishable data in WB in mouse BLCLsPublishable data in WB in mouse BLCLs. -SCBT Publication Review
Date published: 2015-01-30
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CD40 抗体 (LOB-11) is rated 4.0 out of 5 by 8.
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