Date published: 2025-9-7
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CD40 Antibody (H-10) - Western Blotting - Image 354456
CD40 抗体 (H-10): sc-13128. GA-10 (A) 和 Raji (B) 全细胞裂解液中 CD40 表达的 Western 印迹分析.
CD40 抗体 (H-10): sc-13128. GA-10 (A) 和 Raji (B) 全细胞裂解液中 CD40 表达的 Western 印迹分析.

CD40 抗体 (H-10): sc-13128

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说明书
  • CD40 抗体 H-10 是小鼠单克隆 IgG2b κ，CD40抗体, 在18篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CD40的氨基酸74-193
  • CD40 抗体 (H-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 CD40
  • 抗CD40抗体（H-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD40 (H-10): sc-13128的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 CD40 抗体 (H-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD40 抗体 (H-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

CD40抗体（H-10）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测人类CD40蛋白。抗CD40抗体（H-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。CD40在免疫反应中起着至关重要的作用，主要作为共刺激信号，对B细胞活化和分化至关重要。CD40位于B细胞表面，与活化T细胞上表达的CD40配体（CD40L）相互作用，促进B细胞增殖和免疫球蛋白类转换所需的通信。这种相互作用对于产生有效的抗体反应至关重要，特别是在T细胞依赖性抗原的情况下。CD40参与多种免疫过程，包括促进记忆B细胞形成和增强抗体亲和力成熟，这凸显了CD40在免疫调节中的重要性。CD40信号传导失调可导致免疫缺陷或自身免疫性疾病，因此CD40成为治疗干预的重要靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD40 抗体 (H-10) 参考文献:

  1. CD40-CD40配体系统：动脉粥样硬化的潜在治疗靶点。  |  Phipps, RP., et al. 2001. Curr Opin Investig Drugs. 2: 773-7. PMID: 11572655
  2. 血小板活化和CD40/CD40配体通路：人类疾病的机制和影响。  |  Danese, S. and Fiocchi, C. 2005. Crit Rev Immunol. 25: 103-21. PMID: 15952932
  3. 通过靶向VEGF和CD40重新编程巨噬细胞，增强OX40免疫疗法。  |  Liu, Y., et al. 2024. Biochem Biophys Res Commun. 698: 149546. PMID: 38266314
  4. 利什曼原虫MAPK4拦截并重新定向CD40信号，促进感染。  |  Kumari, S., et al. 2024. Int Immunopharmacol. 134: 112100. PMID: 38728877
  5. 一种新型 RING 手指蛋白与 CD40 的细胞质结构域相互作用。  |  Hu, HM., et al. 1994. J Biol Chem. 269: 30069-72. PMID: 7527023
  6. CRAF1是TRAF的亲戚，它参与了CD40信号传导。  |  Cheng, G., et al. 1995. Science. 267: 1494-8. PMID: 7533327
  7. 辅助 T 细胞膜激活 B 细胞。  |  Kehry, MR. and Hodgkin, PD. 1994. Crit Rev Immunol. 14: 221-38. PMID: 7538767
  8. CD40 及其配体：B 淋巴细胞生存, 生长和分化的核心角色。  |  Gordon, J. 1995. Blood Rev. 9: 53-6. PMID: 7540904
  9. T 淋巴细胞中 CD40 配体的表达和 IgE 同型转换的诱导。  |  Fuleihan, R., et al. 1995. Int Arch Allergy Immunol. 107: 43-4. PMID: 7542098
  10. TNF 受体 2 和 CD40 介导的 TRAF2 激活 NF-kappa B。  |  Rothe, M., et al. 1995. Science. 269: 1424-7. PMID: 7544915
  11. 与 75 kDa 肿瘤坏死因子受体胞质结构域缔合作用的新型假定信号转导子家族。  |  Rothe, M., et al. 1994. Cell. 78: 681-92. PMID: 8069916
  12. 在小鼠 B 细胞分化过程中获得 CD40 表达。  |  Grandien, A., et al. 1996. Scand J Immunol. 43: 47-55. PMID: 8560196

订购信息

产品名称产品编号规格价格数量收藏夹

CD40 抗体 (H-10)

sc-13128
200 µg/ml
$316.00

CD40 (H-10): m-IgGκ BP-HRP 套装

sc-520599
200 µg Ab, 40 µg BP
$354.00

CD40 (H-10): m-IgG2b BP-HRP 套装

sc-548842
200 µg Ab; 10 µg BP
$354.00

CD40 抗体 (H-10) AC

sc-13128 AC
500 µg/ml, 25% agarose
$416.00

CD40 抗体 (H-10) HRP

sc-13128 HRP
200 µg/ml
$316.00

CD40 抗体 (H-10) FITC

sc-13128 FITC
200 µg/ml
$330.00

CD40 抗体 (H-10) PE

sc-13128 PE
200 µg/ml
$343.00

CD40 抗体 (H-10) Alexa Fluor® 488

sc-13128 AF488
200 µg/ml
$357.00

CD40 抗体 (H-10) Alexa Fluor® 546

sc-13128 AF546
200 µg/ml
$357.00

CD40 抗体 (H-10) Alexa Fluor® 594

sc-13128 AF594
200 µg/ml
$357.00

CD40 抗体 (H-10) Alexa Fluor® 647

sc-13128 AF647
200 µg/ml
$357.00

CD40 抗体 (H-10) Alexa Fluor® 680

sc-13128 AF680
200 µg/ml
$357.00

CD40 抗体 (H-10) Alexa Fluor® 790

sc-13128 AF790
200 µg/ml
$357.00

Can CD40 Antibody (H-10) antibody be used in western blot or IHC of rat tissue?

Asked by: H_Raphael
Thank you for your question. We do not yet have any data or product citations using this antibody in rat samples, and so it is not a recommended species at this time. As an alternative, we would recommend using, CD40 Antibody (HM40-3): sc-20010.
Answered by: Technical Support
Date published: 2019-07-26

Can CD40 (H-10): sc-13128 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD40 (H-10): sc-13128 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody!This antibody works great on paraffin embeded tissue!
Date published: 2018-07-12
Rated 1 out of 5 by from Only detects albuminI tried this antibody for multiple samples to detect EVs. It only detects albumin and the is absolutely no band for CD40.
Date published: 2018-03-13
Rated 4 out of 5 by from stainsstains some bv in PFA-fixed paraffin-embedded human retina sections
Date published: 2018-02-15
Rated 5 out of 5 by from Good results in WBIt works very well in WB at 1:1000 dilution. I will always use it
Date published: 2017-07-12
Rated 2 out of 5 by from Good for cell IF stainingThis AB showed specific staining in cell's cytoplasm.
Date published: 2017-07-07
Rated 1 out of 5 by from does not work on macaque tissueantibody did not detect CD40 in paraffin embedded macaque retina tissue when used at a dilution of 1:50 ON.
Date published: 2016-12-20
Rated 5 out of 5 by from Published data for WB in HUVECsPublished data for WB in HUVECs. -SCBT Publication Review
Date published: 2015-06-22
Rated 5 out of 5 by from WB positive with ECVWB positive with ECV-304 cells. -SCBT Publication Review
Date published: 2015-04-17
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CD40 抗体 (H-10) is rated 3.9 out of 5 by 13.
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