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CD206/Mannose Receptor/MMR Antibody (15-2) - Western Blotting - Image 15774
CD206 抗体 (15-2): sc-58986. 人肝脏组织提取物中 CD206 表达的 Western 印迹分析.
CD206/Mannose Receptor/MMR Antibody (15-2) - Western Blotting - Image 15774
CD206 抗体 (15-2): sc-58986. 人肝脏组织提取物中 CD206 表达的 Western 印迹分析.
CD206/Mannose Receptor/MMR Antibody (15-2) - Western Blotting - Image 57995
CD206 抗体 (15-2): sc-58986. 人肝脏组织提取物中 CD206 表达的 Western 印迹分析.
CD206 抗体 (15-2): sc-58986. 人肝脏组织提取物中 CD206 表达的 Western 印迹分析.

CD206/Mannose Receptor/MMR 抗体 (15-2): sc-58986

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寫評論提問

说明书
  • CD206/Mannose Receptor/MMR抗体(15-2)是小鼠单克隆IgG1 κ, 在79篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CD206
  • 抗-CD206/Mannose Receptor/MMR 抗体 (15-2) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 CD206
  • 抗CD206/Mannose Receptor/MMR抗体（15-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD206/Mannose Receptor/MMR (15-2): sc-58986的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是CD206/Mannose Receptor/MMR Antibody (15-2) for WB applications. 的首选辅助检测试剂，这些试剂现与CD206/Mannose Receptor/MMR Antibody (15-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD206/甘露糖受体/MMR抗体（15-2）是一种IgG1 κ小鼠单克隆CD206抗体（也称为MRC1抗体），可通过WB、IP、IF和FCM检测小鼠、大鼠和人源的CD206。CD206/甘露糖受体/MMR抗体（15-2）既可以是非偶联的抗CD206抗体形式，也可以是多种偶联形式的抗CD206抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CD206，也称为巨噬细胞甘露糖受体C型（MMR或MRC1），是一种I型膜受体蛋白。它是一种吞噬和胞吞受体，可以识别靶分子中的碳水化合物配体。该蛋白的胞外部分包括八个C型碳水化合物识别域（CRD），它们聚集在一起以实现与糖类的更高亲和力结合。CD206存在于巨噬细胞和肝细胞上，是目前已知的唯一一个含有多个C型CRD的C型凝集素。CD206介导巨噬细胞对糖蛋白的内吞作用，并与潜在致病病毒、真菌和细菌表面的高甘露糖结构结合，使它们能够通过吞噬作用被中和。在炎症期间，CD206对于快速清除几种含甘露糖的血清糖蛋白至关重要，但不调节炎症的启动。CD206主要在成熟组织巨噬细胞、未成熟树突状细胞以及肝和淋巴内皮细胞、视网膜色素上皮（RPE）和系膜细胞中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD206/Mannose Receptor/MMR 抗体 (15-2)

sc-58986
200 µg/ml
$316.00

CD206/Mannose Receptor/MMR (15-2): m-IgG Fc BP-HRP 套装

sc-526922
200 µg Ab; 10 µg BP
$354.00

CD206/Mannose Receptor/MMR (15-2): m-IgG1 BP-HRP 套装

sc-532295
200 µg Ab; 20 µg BP
$354.00

CD206/Mannose Receptor/MMR 抗体 (15-2) AC

sc-58986 AC
500 µg/ml, 25% agarose
$416.00

CD206/Mannose Receptor/MMR 抗体 (15-2) HRP

sc-58986 HRP
200 µg/ml
$316.00

CD206/Mannose Receptor/MMR 抗体 (15-2) FITC

sc-58986 FITC
200 µg/ml
$330.00

CD206/Mannose Receptor/MMR 抗体 (15-2) PE

sc-58986 PE
200 µg/ml
$343.00

CD206/Mannose Receptor/MMR 抗体 (15-2) Alexa Fluor® 488

sc-58986 AF488
200 µg/ml
$357.00

CD206/Mannose Receptor/MMR 抗体 (15-2) Alexa Fluor® 546

sc-58986 AF546
200 µg/ml
$357.00

CD206/Mannose Receptor/MMR 抗体 (15-2) Alexa Fluor® 594

sc-58986 AF594
200 µg/ml
$357.00

CD206/Mannose Receptor/MMR 抗体 (15-2) Alexa Fluor® 647

sc-58986 AF647
200 µg/ml
$357.00

CD206/Mannose Receptor/MMR 抗体 (15-2) Alexa Fluor® 680

sc-58986 AF680
200 µg/ml
$357.00

CD206/Mannose Receptor/MMR 抗体 (15-2) Alexa Fluor® 790

sc-58986 AF790
200 µg/ml
$357.00

What is the recommended starting dilution for IHC for sc-58986: CD206 (15-2) monoclonal antibody?

Asked by: zxcvbbnm
Thank you for your question.This antibody can be used for frozen samples and paraffin section is not currently a recommended application. The specific dilution:immunofluorescence or immunohistochemistry is starting dilution 1:50, dilution range 1:50-1:500.If you have any further questions, please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website. Thank you.
Answered by: Jiajun Z
Date published: 2022-11-07

Can this antibody functionally block mannose binding?

Asked by: hwash
Thank you for your question. Anti-CD206/Mannose Receptor/MMR Antibody (15-2) is intended and recommended for detection of CD206 of mouse, rat and human origin by WB, IP, IF and FCM. It has not been tested for its blocking properties and we do not have any data to support such an assay.
Answered by: Tech Support Europe
Date published: 2022-09-05

您好，想做大鼠石蜡切片的免疫荧光双标， CD206 Antibody (D-1): sc-376108 and CD206 Antibody (15-2): sc-58986 两个抗体都能用吗，哪个更合适呢？

Asked by: xiaoyounvzi
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2023-10-15

您好！请问CD206抗体（SC-58986）是否可以用于M2型小胶质细胞的免疫组化和免疫荧光染色？

Asked by: PURPLE
感谢您的提问。请发邮件至asia@scbio.cn，联系我们的技术服务部门。 Thank you for your question. Please contact our Technical Service Department at asia@scbio.cn.
Answered by: Technical Support 12
Date published: 2021-07-07

Can this Ab (sc-58986: CD206 (15-2)) is good for IP? What will be the recommended dilution for IP?

Asked by: Apricano
Thank you for your question. Anti-CD206 Antibody (15-2) is recommended for detection of CD206 of mouse, rat and human origin by WB, IP, IF and FCM. For IP we recommend 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) You can find our recommended protocol for IP under this link: https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: Tech Support Europe
Date published: 2020-12-08

Can I use sc-58986 for indirect flow cytometry in mouse and rat? If I can, do you have a recommended fluorescent secondary antibody?

Asked by: ykwq
Thank you for your question. This antibody is recommended for flow cytometry (FCM) in mouse, rat and human samples. We have not specifically tested this antibody in indirect flow cytometry, but if you want to try it we would recommend using anti-mouse IgG1 for detection. One example would be our m-IgGκ BP-CFL 488: sc-516176.
Answered by: Technical Support
Date published: 2019-04-13

Can I use it to do Immunofluorescence double staining？My sample is from Mouse Ovary and Endometrium Tissue and I am afraid it can not be stained well.

Asked by: Puff Daddy
Thank you for your question. Yes, this antibody is mouse reactive and should be fine for double straining on mouse samples.
Answered by: Technical Support
Date published: 2019-01-05

Hi, do you have the CD206 antibody (15-2) conjugated to AF 647 for flow cytometry? 

Asked by: Varsha
Thank you for your question. Yes, we offer this antibody conjugated to AlexaFluor 647. The catalog number is: sc-58986 AF647.
Answered by: Tech Service
Date published: 2017-12-06
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Rated 5 out of 5 by from Good antibodyThis antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 3 out of 5 by from not clear bandwe used this sample to do the western blot ,the sample is the Mouse monoclonal antibody .we used this sample to dectect the CD206 of RAW246.7 cell.we detected that there are no bands at approximately 170 kDa .
Date published: 2017-11-20
Rated 4 out of 5 by from clear bandwe used this sample to do the western blot ,the sample is the Mouse monoclonal antibody .we used this sample to dectect the B7-2 of RAW246.7 cell.we detected that the band of B7-2 was approximately 66 kDa .
Date published: 2017-10-12
Rated 4 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of CD206 expression in human liver tissue extract. -SCBT QC
Date published: 2015-03-08
Rated 4 out of 5 by from SingleSingle, strong band in western blot analysis of CD206 expression in human liver tissue extract. -SCBT QC
Date published: 2015-02-12
Rated 4 out of 5 by from Clean IF staining in human THPClean IF staining in human THP-1 cells. -SCBT Publication Review
Date published: 2015-01-26
Rated 4 out of 5 by from Published WB/IF using human cultured PBMCsPublished WB/IF using human cultured PBMCs. -SCBT Publication Review
Date published: 2013-08-08
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CD206/Mannose Receptor/MMR 抗体 (15-2) is rated 4.0 out of 5 by 7.
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