Date published: 2025-9-8
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C23 (Nucleolin) Antibody (D-6) - Western Blotting - Image 373035
C23 抗体 (D-6): sc-17826. MOLT-4 (A) 和 Raji (B) 全细胞裂解液中 C23 表达的 Western 印迹分析.
C23 抗体 (D-6): sc-17826. MOLT-4 (A) 和 Raji (B) 全细胞裂解液中 C23 表达的 Western 印迹分析.

C23 (Nucleolin) 抗体 (D-6): sc-17826

4.4(12)
寫評論提問

说明书
  • C23 (Nucleolin)抗体(D-6)是小鼠单克隆IgG2a κ, 在43篇文献中引用，规格为200 µg/ml
  • 免疫human物种的C23的氨基酸271-520
  • 抗-C23 (Nucleolin) 抗体 (D-6) 推荐用于 WB, IP, IF 和 IHC(P)，检测human 和monkey 来源的 C23
  • 抗C23 (Nucleolin)抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取C23 (Nucleolin) (D-6): sc-17826的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 C23 (Nucleolin) 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与C23 (Nucleolin) 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

C23抗体（D-6）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测人类和猴源性C23。C23 (D-6) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。C23 也称为核仁蛋白 (NCL)，是一种重要的真核核仁磷蛋白，在核糖体合成和成熟过程中发挥着重要作用。C23主要位于核仁的致密纤维区域，含有四个RNA结合域，在核糖体生物发生过程中促进与前核糖体RNA的相互作用。这种定位使C23能够影响RNA处理、核糖体基因转录和核仁中核糖体成分的定位，从而确保核糖体的有效组装和功能。C23与多种蛋白质相互作用，包括核仁蛋白B23，而Cdc2和酪蛋白激酶II的磷酸化作用则导致其从核仁转移到细胞质，凸显其在细胞过程中的动态作用。C23与人类端粒酶逆转录酶亚基（TERT）的相互作用尤其值得注意，因为它是TERT定位到核仁的关键，将C23与细胞衰老和癌症的关键途径联系起来。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

C23 抗体 (D-6)

sc-17826
200 µg/ml
$316.00

C23 (Nucleolin) (D-6): m-IgG Fc BP-HRP 套装

sc-528326
200 µg Ab; 10 µg BP
$354.00

C23 (Nucleolin) (D-6): m-IgGκ BP-HRP 套装

sc-520672
200 µg Ab, 40 µg BP
$354.00

C23 (Nucleolin) (D-6): m-IgG2a BP-HRP 套装

sc-547073
200 µg Ab; 10 µg BP
$354.00

C23 (Nucleolin) 抗体 (D-6) AC

sc-17826 AC
500 µg/ml, 25% agarose
$416.00

C23 (Nucleolin) 抗体 (D-6) HRP

sc-17826 HRP
200 µg/ml
$316.00

C23 (Nucleolin) 抗体 (D-6) FITC

sc-17826 FITC
200 µg/ml
$330.00

C23 (Nucleolin) 抗体 (D-6) PE

sc-17826 PE
200 µg/ml
$343.00

C23 (Nucleolin) 抗体 (D-6) Alexa Fluor® 488

sc-17826 AF488
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (D-6) Alexa Fluor® 546

sc-17826 AF546
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (D-6) Alexa Fluor® 594

sc-17826 AF594
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (D-6) Alexa Fluor® 647

sc-17826 AF647
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (D-6) Alexa Fluor® 680

sc-17826 AF680
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (D-6) Alexa Fluor® 790

sc-17826 AF790
200 µg/ml
$357.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-02-03
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Rated 3 out of 5 by from AcceptableStrong signal in WB with whole cell extracts from Jurkat and k562 cells. However, co-detection of several additional bands.
Date published: 2024-05-01
Rated 1 out of 5 by from Did not work on ICCTried on Hela cells for immunocytochemistry, it stains the entire cells.
Date published: 2018-06-20
Rated 5 out of 5 by from Great antibody!The antibody worked great identifying the target from HEK cells at 1:1000 dilution.
Date published: 2018-04-27
Rated 5 out of 5 by from Thanks!Great antibody! I will buy more antibody from santa antibody.
Date published: 2017-11-27
Rated 5 out of 5 by from Great C23 antibodyPublished, well-tested antibody that performs well in Western blot, IF, and IHC(P). Recommended for human and monkey detection--tested negative in mouse NIH/3T3 cells.
Date published: 2017-02-03
Rated 5 out of 5 by from GoodGood, clear band obtained in WB in Jurkat nuclear extract. -SCBT QC
Date published: 2015-04-29
Rated 5 out of 5 by from Positive band seen in WB in 293T whole cellPositive band seen in WB in 293T whole cell lysate and HeLa nuclear extract. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Published data for WB in monkey Cos7 cellsPublished data for WB in monkey Cos7 cells. -SCBT Publication Review
Date published: 2015-03-29
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C23 (Nucleolin) 抗体 (D-6) is rated 4.4 out of 5 by 12.
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