C23抗体 (MS-3) 推荐用于 mouse, rat 和 human 来源的C23 WB, IP, IF, IHC(P), FCM 和 ELISA检测

C23 抗体 (MS-3): sc-8031

C23抗体 (MS-3) is rated 4.8 out of 5 by 28.
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说明书

    • 抗-C23 抗体 MS-3 是小鼠单克隆 IgG1 κ,C23抗体, 在284篇文献中引用,规格为200 µg/ml
    • 免疫1-706氨基酸,对应于human来源C23全长
    • 抗-C23 抗体 (MS-3) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA,检测mouse, rat 和human 来源的 C23
    • 抗-C23 抗体 (MS-3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
    • 关于如何获取C23 (MS-3): sc-8031免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for C23 Antibody (MS-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with C23 Antibody (MS-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Did anyone tried C23-conjugated to agarose for IP? If yes, can you please share or refer me to a paper for the IP protocol?

Asked by: ECRM
Thank you for your question. There are currently 22 product citations using this antibody for IP. It is not known if any of those publications specifically used the agarose-conjugated version of this antibody. However, you can find these application-specific citations by using the Product Citation Search on our website (under the Resources menu at the bottom of the page). You can also refer to our general IP protocol for recommendations on using our agarose-conjugated antibodies. https://www.scbt.com/scbt/misc/citationSearch.jsp
Answered by: Technical Support
Date published: 2019-06-16

Can you purchase MS-3 as a dry powder and resuspend it in your own buffer?

Asked by: PeterM
Thank you for your question. The regular formulation of the C23 Antibody (MS-3) is provided at 200 µg/ml in PBS with 0.1 % gelatin and <0.1 % sodium azide. If you require a custom formulation of this antibody, please get in touch with our technical service.
Answered by: Tech Support Europe
Date published: 2019-06-04

Hi, I have ordered the C23 (MS-3) Alexafluor 488 antibody, however, I am a little confused about the IF protocol to be followed. Could you please provide me a protocol to use the antibody?

Asked by: Sravani
Thank you for your question. A link to our recommended protocol for using this mouse monoclonal, C23 Antibody (MS-3): sc-8031, for immunofluorescence. Please refer to the very end of the protocol, section "E. Direct Immunofluorescence Staining" for using the directly conjugated AF488 primary antibody. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-04-17

I work mostly with mouse tissues and want to use this antibody for an IHC stain. How can I use a mouse monoclonal to stain mouse tissue?

Asked by: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, sc-8031, is available conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2016-12-23

Dear officer: I ordered sc-8031. NCL antibody (C23 Ms-3), I want to do immunoprecipitation with this antibody, and want to use NCL peptide to elute the immunopreciptated products out, is there any chance I can get some NCL peptide from you? Manhong

Asked by: Manhong Guo
Thank you for your question. Our C23 (MS-3) antibody, sc-8031, was raised against amino acids 1-706 representing full length C23 of human origin. We do not have a peptide available for elution.
Answered by: Technical Service
Date published: 2016-10-24
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Rated 1 out of 5 by from Non funziona Ho comprato un mese fa questo anticorpo e non funziona per western blot
Date published: 2020-05-29
Rated 5 out of 5 by from sc-8031 sc-8031 is works great for western blot with Balb/c mice.
Date published: 2018-11-20
Rated 5 out of 5 by from Muy buen anticuerpo para inmunohistoquímica Buena sensibilidad y especificidad en inmunohistoquímicas de tejidos parafinados
Date published: 2017-12-11
Rated 4 out of 5 by from Good for western blot! It detected endogenous nucleolin in HEK 293T cells (1:500 dilution in 5% milk in TBS-0.1% Tween, overnight at 4C). Recommended!
Date published: 2017-11-02
Rated 5 out of 5 by from Western blot Western blot analysis target protein C23 in mouse brain tissue lysates. Antibody dilution used 1:800 in 5% milk incubated for 2 hour at RT, The Gel was used 8% SDS PAGE,The target band at 110kda.
Date published: 2017-09-09
Rated 5 out of 5 by from good assay result This antibody used to detect C23 protein.We loaded 60ug mouse kidney tissue lysates , Very easy to got the target protein band 110kda.
Date published: 2017-09-09
Rated 5 out of 5 by from Great antibody Great antibody! Recognized specifically protein C23 in Western blot,the specific band at 110 kDa (the expected size) in lysates from HEK293 cells,clear singal.
Date published: 2017-08-12
Rated 5 out of 5 by from strong signal We are interested in assessing the levels of certain C23 protein in epithelial tissue. We choose this antibody because the images on santa's website looked convincing (in K-562). I got very strong band at 1:500 dilution.
Date published: 2017-07-29
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