Date published: 2025-9-7
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C23 (Nucleolin) Antibody (MS-3) - Western Blotting - Image 354358
C23 抗体 (MS-3) HRP: sc-8031 HRP. Jurkat (A), CCRF-CEM (B) 和 K-562 (C) 全细胞裂解液中 C23 表达的直接 Western 印迹分析.
C23 抗体 (MS-3) HRP: sc-8031 HRP. Jurkat (A), CCRF-CEM (B) 和 K-562 (C) 全细胞裂解液中 C23 表达的直接 Western 印迹分析.

C23 (Nucleolin) 抗体 (MS-3): sc-8031

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寫評論提問

说明书
  • C23 (Nucleolin) 抗体 MS-3 是小鼠单克隆 IgG1 κ，C23 (Nucleolin)抗体, 在327篇文献中引用，规格为200 µg/ml
  • 免疫1-706氨基酸，对应于human来源C23全长
  • C23 (Nucleolin) 抗体 (MS-3) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 C23
  • 抗C23 (Nucleolin)抗体（MS-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取C23 (Nucleolin) (MS-3): sc-8031的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 C23 (Nucleolin) 抗体 (MS-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与C23 (Nucleolin) 抗体 (MS-3) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
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说明书与实验方案
研究信息

関連項目

C23抗体（MS-3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的C23蛋白。C23单克隆抗体（MS-3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。C23，也称为核仁蛋白（NCL），是一种重要的真核细胞核仁磷蛋白，在核糖体的合成和成熟过程中发挥着重要作用，而核糖体对于所有活细胞中的蛋白质合成至关重要。C23主要位于核仁的致密纤维区域，其中C23包含四个RNA结合域，有助于在核糖体生物发生过程中与前核糖体RNA相互作用。这种定位确保了核糖体RNA的高效处理和核糖体亚基的组装，而核糖体亚基是细胞功能和生长所必需的。C23与多种蛋白质相互作用，包括核仁蛋白B23，而Cdc2和酪蛋白激酶II的磷酸化作用会导致其从核仁转移到细胞质，从而凸显其在细胞过程中的动态作用。C23与人类端粒酶逆转录酶亚基（TERT）的相互作用对于TERT的核仁定位尤为重要，进一步强调了其在细胞调控和稳定性中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

C23 (Nucleolin) 抗体 (MS-3) 参考文献:

  1. 核仁蛋白与端粒酶相互作用。  |  Khurts, S., et al. 2004. J Biol Chem. 279: 51508-15. PMID: 15371412
  2. 非磷酸化的人La抗原与核蛋白在参与rRNA生物发生的核小体位点相互作用。  |  Intine, RV., et al. 2004. Mol Cell Biol. 24: 10894-904. PMID: 15572691
  3. 有丝分裂中 Bcl-2 磷酸化形式的定域：与 Ki-67 和 nucleolin 共同定位于核结构和有丝分裂染色体上。  |  Barboule, N., et al. 2005. Cell Cycle. 4: 590-6. PMID: 15876860
  4. p34cdc2蛋白激酶对核仁蛋白有丝分裂特异性磷酸化作用  |  Belenguer, P., et al. 1990. Mol Cell Biol. 10: 3607-18. PMID: 2192260
  5. C23 通过抑制 p53 的活性来促进肿瘤发生。  |  Li, Q., et al. 2016. Oncotarget. 7: 58274-58285. PMID: 27506938
  6. 抗 C23（核蛋白）抗体对摇蚊唾液腺细胞核糖体 DNA 转录的影响  |  Egyhazi, E., et al. 1988. Exp Cell Res. 178: 264-72. PMID: 3169130
  7. 位于核仁蛋白（C23 和 100 kDa）羧基末端附近的富含甘氨酸, 二甲基精氨酸区域的蛋白质和 cDNA 序列。  |  Lapeyre, B., et al. 1986. J Biol Chem. 261: 9167-73. PMID: 3755137
  8. 磷蛋白 C23 在核极结构和核仁组织区的定位  |  Lischwe, MA., et al. 1981. Exp Cell Res. 136: 101-9. PMID: 7028492
  9. C23 与 B23（一种假定的核定位信号结合蛋白）相互作用。  |  Li, YP., et al. 1996. Eur J Biochem. 237: 153-8. PMID: 8620867
  10. Nucleolin 在核糖体 RNA 处理的第一步中发挥作用。  |  Ginisty, H., et al. 1998. EMBO J. 17: 1476-86. PMID: 9482744
  11. 一种 B 细胞特异性 DNA 重组复合体。  |  Borggrefe, T., et al. 1998. J Biol Chem. 273: 17025-35. PMID: 9642267
  12. 核蛋白通过其 RGG 结构域与几种核糖体蛋白相互作用。  |  Bouvet, P., et al. 1998. J Biol Chem. 273: 19025-9. PMID: 9668083

订购信息

产品名称产品编号规格价格数量收藏夹

C23 抗体 (MS-3)

sc-8031
200 µg/ml
$316.00

C23 (Nucleolin) (MS-3): m-IgGκ BP-HRP 套装

sc-520531
200 µg Ab, 40 µg BP
$354.00

C23 (Nucleolin) 抗体 (MS-3) AC

sc-8031 AC
500 µg/ml, 25% agarose
$416.00

C23 (Nucleolin) 抗体 (MS-3) HRP

sc-8031 HRP
200 µg/ml
$316.00

C23 (Nucleolin) 抗体 (MS-3) FITC

sc-8031 FITC
200 µg/ml
$330.00

C23 (Nucleolin) 抗体 (MS-3) PE

sc-8031 PE
200 µg/ml
$343.00

C23 (Nucleolin) 抗体 (MS-3) Alexa Fluor® 488

sc-8031 AF488
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (MS-3) Alexa Fluor® 546

sc-8031 AF546
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (MS-3) Alexa Fluor® 594

sc-8031 AF594
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (MS-3) Alexa Fluor® 647

sc-8031 AF647
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (MS-3) Alexa Fluor® 680

sc-8031 AF680
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (MS-3) Alexa Fluor® 790

sc-8031 AF790
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (MS-3) TRITC

sc-8031 TRITC
200 µg/ml
$320.00

Did anyone tried C23-conjugated to agarose for IP? If yes, can you please share or refer me to a paper for the IP protocol?

Asked by: ECRM
Thank you for your question. There are currently 22 product citations using this antibody for IP. It is not known if any of those publications specifically used the agarose-conjugated version of this antibody. However, you can find these application-specific citations by using the Product Citation Search on our website (under the Resources menu at the bottom of the page). You can also refer to our general IP protocol for recommendations on using our agarose-conjugated antibodies. https://www.scbt.com/scbt/misc/citationSearch.jsp
Answered by: Technical Support
Date published: 2019-06-16

Can you purchase MS-3 as a dry powder and resuspend it in your own buffer?

Asked by: PeterM
Thank you for your question. The regular formulation of the C23 Antibody (MS-3) is provided at 200 µg/ml in PBS with 0.1 % gelatin and <0.1 % sodium azide. If you require a custom formulation of this antibody, please get in touch with our technical service.
Answered by: Tech Support Europe
Date published: 2019-06-04

Hi, I have ordered the C23 (MS-3) Alexafluor 488 antibody, however, I am a little confused about the IF protocol to be followed. Could you please provide me a protocol to use the antibody?

Asked by: Sravani
Thank you for your question. A link to our recommended protocol for using this mouse monoclonal, C23 Antibody (MS-3): sc-8031, for immunofluorescence. Please refer to the very end of the protocol, section "E. Direct Immunofluorescence Staining" for using the directly conjugated AF488 primary antibody. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-04-17

I work mostly with mouse tissues and want to use this antibody for an IHC stain. How can I use a mouse monoclonal to stain mouse tissue?

Asked by: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, sc-8031, is available conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2016-12-23

Dear officer: I ordered sc-8031. NCL antibody (C23 Ms-3), I want to do immunoprecipitation with this antibody, and want to use NCL peptide to elute the immunopreciptated products out, is there any chance I can get some NCL peptide from you? Manhong

Asked by: Manhong Guo
Thank you for your question. Our C23 (MS-3) antibody, sc-8031, was raised against amino acids 1-706 representing full length C23 of human origin. We do not have a peptide available for elution.
Answered by: Technical Service
Date published: 2016-10-24
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Rated 5 out of 5 by from WesterblotAntibody works very well in Westerblot (1:500 dilution) in HEK RIPA lysate.
Date published: 2023-02-06
Rated 1 out of 5 by from Non funzionaHo comprato un mese fa questo anticorpo e non funziona per western blot
Date published: 2020-05-29
Rated 5 out of 5 by from sc-8031sc-8031 is works great for western blot with Balb/c mice.
Date published: 2018-11-20
Rated 5 out of 5 by from Muy buen anticuerpo para inmunohistoquímicaBuena sensibilidad y especificidad en inmunohistoquímicas de tejidos parafinados
Date published: 2017-12-11
Rated 4 out of 5 by from Good for western blot!It detected endogenous nucleolin in HEK 293T cells (1:500 dilution in 5% milk in TBS-0.1% Tween, overnight at 4C). Recommended!
Date published: 2017-11-02
Rated 5 out of 5 by from Western blotWestern blot analysis target protein C23 in mouse brain tissue lysates. Antibody dilution used 1:800 in 5% milk incubated for 2 hour at RT, The Gel was used 8% SDS PAGE,The target band at 110kda.
Date published: 2017-09-09
Rated 5 out of 5 by from good assay resultThis antibody used to detect C23 protein.We loaded 60ug mouse kidney tissue lysates , Very easy to got the target protein band 110kda.
Date published: 2017-09-09
Rated 5 out of 5 by from Great antibodyGreat antibody! Recognized specifically protein C23 in Western blot,the specific band at 110 kDa (the expected size) in lysates from HEK293 cells,clear singal.
Date published: 2017-08-12
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C23 (Nucleolin) 抗体 (MS-3) is rated 4.8 out of 5 by 29.
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