Date published: 2025-9-7

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BRCA1 抗体 (287.17): sc-135732

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说明书
  • BRCA1抗体(287.17)是小鼠单克隆IgG2b κ, 在11篇文献中引用,规格为200 µg/ml
  • 免疫mouse物种的重组BRCA1
  • 推荐用于 mouse, rat 和 human 来源的BRCA1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 BRCA1 (247.56): sc-135731,可查看有标记的 BRCA1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG2b BP-HRP 是BRCA1 抗体 (287.17) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与BRCA1 抗体 (287.17) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

1990年,一个乳腺癌症易感基因,命名为BRCA1,定位于染色体17q。据信,该基因突变约占癌症高发家庭的45%,以及早发性癌症和癌症发病率增加家庭的至少80%。位于染色体13q13.1上的第二个癌症易感性基因BRCA2也会导致癌症的高发病率,但与BRCA1不同,它不会导致卵巢癌症的风险显著升高。BRCA1基因在包括乳腺和卵巢在内的许多组织中表达,并编码一个预测的1863个氨基酸的蛋白质。该蛋白质在其氨基末端区域包含一个锌指结构域,但与之前描述的任何蛋白质无关。像许多其他参与家族性癌症的基因一样,BRCA1似乎编码一种肿瘤抑制因子,一种对肿瘤生长起负调控作用的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

BRCA1 抗体 (287.17)

sc-135732
200 µg/ml
$316.00

BRCA1 (287.17): m-IgG2b BP-HRP 套装

sc-548493
200 µg Ab; 10 µg BP
$354.00

Dear Sir/Madam, Could you possibly provide me with the exact epitopes of this antibody?

Asked by: Milo Kaptein
Thank you for your question. sc-135732 BRCA1 (287.17) has not been epitope mapped but here is a link for more information. https://www.uniprot.org/uniprotkb/P48754/entry
Answered by: BlakeJ
Date published: 2025-01-08

Where is the immuogen of the antibody?

Asked by: AAAAA
Thank you for your question. Please contact technical service by email at scbt@scbt.com
Answered by: BlakeJ
Date published: 2023-11-02

I see two bands, one at the height of 250kDa and the other at 230kDa, which is true? The 250 is better delineated at WB. However, I cannot evaluate the genome sequence of a mouse-derived cell with confirmed BRCA KO because the band appears at 250 kDa.

Asked by: gaisan
We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please reach out to your local distributor in Japan or contact Technical Service directly. We are happy to help you optimize and troubleshoot the antibody to make sure you have success with the antibody.
Answered by: SCBT Representative
Date published: 2023-12-18

I am using BRCA1 (287.17): sc-135732 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Produced wonderful immunoperoxidase nuclearProduced wonderful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human vulva/anal skin tissue. -SCBT QC
Date published: 2013-01-10
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BRCA1 抗体 (287.17) is rated 4.0 out of 5 by 1.
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