NUAK1/ARK5 Antibody (B-4): sc-271828
- NUAK1/ARK5 Antibody (B-4) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 526-661 mapping at the C-terminus of NUAK1 of human origin
- NUAK1/ARK5 Antibody (B-4) is recommended for detection of NUAK1 by WB, IP, IF and ELISA
- Anti-NUAK1/ARK5 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for NUAK1/ARK5 Antibody (B-4) for WB applications. These reagents are now offered in bundles with NUAK1/ARK5 Antibody (B-4) (see ordering information below).
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NUAK1 Antibody (B-4) is a mouse monoclonal IgG2a antibody that detects NUAK1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NUAK1, also known as omphk1 (omphalocele kinase 1), is a 658 amino acid protein located in both the nucleus and cytoplasm, belonging to the CAMK Ser/Thr protein kinase family and the SNF1 subfamily. anti-NUAK1 antibody (B-4) plays a crucial role in studying various cellular processes, including cell adhesion, regulation of cell ploidy and senescence, as well as cell proliferation and tumor progression. NUAK1′s cellular location enables interactions with key signaling pathways that govern these processes, making NUAK1 essential for maintaining cellular homeostasis and responding to stress signals. Notably, NUAK1 phosphorylates several critical proteins, including Atm, caspase-6, LATS1, MYPT1, and p53, which are involved in DNA damage response and apoptosis. NUAK1 expression in the developing central nervous system and epidermis highlights NUAK1′s significance in developmental biology, while homozygous Nauk1 mutants exhibit omphalocele, a serious condition resulting from improper closure of the body wall, underscoring NUAK1′s vital role in embryonic development. With diverse functions and critical interactions, NUAK1 (B-4) antibody serves as an invaluable tool for researchers investigating the molecular mechanisms underlying cell growth, differentiation, and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NUAK1/ARK5 Antibody (B-4) References:
- NUAK1 knockdown suppresses prostate cancer cell epithelial-mesenchymal transition, migration, and invasion through microRNA-30b-5p. | Guan, Y., et al. 2018. Int J Clin Exp Pathol. 11: 5694-5704. PMID: 31949655
- Suppressing the KIF20A/NUAK1/Nrf2/GPX4 signaling pathway induces ferroptosis and enhances the sensitivity of colorectal cancer to oxaliplatin. | Yang, C., et al. 2021. Aging (Albany NY). 13: 13515-13534. PMID: 33819186
- DKK1 promotes NUAK1 transcriptional expression through the activation Akt in hepatocellular carcinoma. | Yao, CY., et al. 2023. Cell Biol Int. 47: 383-393. PMID: 36480792
- NUAK1 governs centrosome replication in pancreatic cancer via MYPT1/PP1β and GSK3β-dependent regulation of PLK4. | Whyte, D., et al. 2023. Mol Oncol. 17: 1212-1227. PMID: 36975767
- NUAK1 promotes tumor metastasis through upregulating slug transcription in esophageal squamous cell carcinoma. | Yang, H., et al. 2023. Cancer Cell Int. 23: 258. PMID: 37919754
- NUAK1 coordinates growth factor-dependent activation of mTORC2 and Akt signaling. | Palma, M., et al. 2023. Cell Biosci. 13: 232. PMID: 38135881
- The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions. | Lanfranchi, M., et al. 2024. Nat Commun. 15: 2487. PMID: 38514619
- NUAK1 promotes metabolic dysfunction-associated steatohepatitis progression by activating Caspase 6-driven pyroptosis and inflammation. | Sheng, M., et al. 2024. Hepatol Commun. 8: PMID: 38967580
- NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer. | Cao, L., et al. 2024. Cell Rep. 43: 114446. PMID: 38996065
- NUAK1-Mediated Phosphorylation of NADK Mitigates ROS Accumulation to Promote Osimertinib Resistance in Non-Small Cell Lung Carcinoma. | Lin, W., et al. 2024. Cancer Res. 84: 4081-4098. PMID: 39159134
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NUAK1/ARK5 Antibody (B-4) | sc-271828 | 200 µg/ml | $322.00 | |||
NUAK1/ARK5 Antibody (B-4): m-IgG Fc BP-HRP Bundle | sc-525828 | 200 µg Ab; 10 µg BP | $361.00 | |||
NUAK1/ARK5 Antibody (B-4): m-IgG2a BP-HRP Bundle | sc-546432 | 200 µg Ab; 10 µg BP | $361.00 | |||
NUAK1/ARK5 Antibody (B-4) AC | sc-271828 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NUAK1/ARK5 Antibody (B-4) HRP | sc-271828 HRP | 200 µg/ml | $322.00 | |||
NUAK1/ARK5 Antibody (B-4) FITC | sc-271828 FITC | 200 µg/ml | $336.00 | |||
NUAK1/ARK5 Antibody (B-4) PE | sc-271828 PE | 200 µg/ml | $349.00 | |||
NUAK1/ARK5 Antibody (B-4) Alexa Fluor® 488 | sc-271828 AF488 | 200 µg/ml | $364.00 | |||
NUAK1/ARK5 Antibody (B-4) Alexa Fluor® 546 | sc-271828 AF546 | 200 µg/ml | $364.00 | |||
NUAK1/ARK5 Antibody (B-4) Alexa Fluor® 594 | sc-271828 AF594 | 200 µg/ml | $364.00 | |||
NUAK1/ARK5 Antibody (B-4) Alexa Fluor® 647 | sc-271828 AF647 | 200 µg/ml | $364.00 | |||
NUAK1/ARK5 Antibody (B-4) Alexa Fluor® 680 | sc-271828 AF680 | 200 µg/ml | $364.00 | |||
NUAK1/ARK5 Antibody (B-4) Alexa Fluor® 790 | sc-271828 AF790 | 200 µg/ml | $364.00 |