Date published: 2025-9-8
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A20/TNFAIP3 Antibody (A-12) - Western Blotting - Image 375103
A20 抗体 (A-12): sc-166692. ACHN (A) 和 Daudi (B) 全细胞裂解液中 A20 表达的 Western 印迹分析.
A20 抗体 (A-12): sc-166692. ACHN (A) 和 Daudi (B) 全细胞裂解液中 A20 表达的 Western 印迹分析.

A20/TNFAIP3 抗体 (A-12): sc-166692

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说明书
  • A20/TNFAIP3 抗体 A-12 是小鼠单克隆 IgG2a κ，A20/TNFAIP3抗体, 在74篇文献中引用，规格为200 µg/ml
  • 免疫human物种的A20的氨基酸1-100
  • A20/TNFAIP3 抗体 (A-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 A20
  • 抗A20/TNFAIP3抗体（A-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-166692 X, 200 µg/0.1 ml)
  • 关于如何获取A20/TNFAIP3 (A-12): sc-166692的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 A20/TNFAIP3 抗体 (A-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与A20/TNFAIP3 抗体 (A-12) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

A20抗体（A-12）是一种IgG2a κ小鼠单克隆A20抗体（也称为TNFAIP3抗体，或TNFα诱导蛋白3抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类的A20蛋白。A20抗体（A-12）既可以非偶联抗A20抗体形式提供，也可以多种偶联形式的抗A20抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。A20是一种Cys2/Cys2锌指蛋白，由多种炎性刺激诱导，并调节基因表达。具体而言，A20由肿瘤坏死因子（TNF）和白细胞介素1（IL-1）诱导，并作为核因子κB（NFκB）基因表达的负调节因子。通过抑制NFκB活化，A20在终止NFκB对各种刺激的反应中起着关键作用。尽管A20的C末端区域包含七个锌指结构域，但体外抑制TNF诱导的NFκB活化只需要其中的四个结构域。A20还与其他几种蛋白质相互作用，如TRAF2、TRAF6和IkB激酶（IKK）γ蛋白，从而可以抑制细胞死亡。TXBP151是一种新型的A20结合蛋白，可能介导A20的抗凋亡活性。A20和A20结合蛋白参与信号转导的负反馈调节，可能作为治疗多种疾病的新型治疗工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

A20/TNFAIP3 抗体 (A-12) 参考文献:

  1. 锌指蛋白 A20 与一种新型抗凋亡蛋白相互作用，这种蛋白会被特定的 Caspases 分解。  |  De Valck, D., et al. 1999. Oncogene. 18: 4182-90. PMID: 10435631
  2. A20 和 A20 结合蛋白作为核因子-kappa B 依赖性基因表达和细胞凋亡的细胞阻抑剂。  |  Beyaert, R., et al. 2000. Biochem Pharmacol. 60: 1143-51. PMID: 11007952
  3. A20 锌指蛋白在信号级联的早期抑制 TNF 诱导的细胞凋亡和应激反应，与 TRAF2 或 14-3-3 蛋白的结合无关。  |  Lademann, U., et al. 2001. Cell Death Differ. 8: 265-72. PMID: 11319609
  4. A20 锌指在抑制 NF-kappaB 激活和蛋白质相互作用方面的功能冗余。  |  Klinkenberg, M., et al. 2001. FEBS Lett. 498: 93-7. PMID: 11389905
  5. 发现一种名为 ABIN-2 的新型 A20 结合型核因子-kappa B 激活抑制剂。  |  Van Huffel, S., et al. 2001. J Biol Chem. 276: 30216-23. PMID: 11390377
  6. 去泛素酶A20在自身免疫性疾病免疫病理学中的作用。  |  Majumdar, I. and Paul, J. 2014. Autoimmunity. 47: 307-19. PMID: 24673262
  7. A20在胰岛生物学和病理学中的新兴作用。  |  Zammit, NW. and Grey, ST. 2014. Adv Exp Med Biol. 809: 141-62. PMID: 25302370
  8. A20磷酸化控制A20功能。  |  Martens, A. and van Loo, G. 2019. Nat Immunol. 20: 1261-1262. PMID: 31534239
  9. A20通过泛素结合限制炎症。  |  Sun, SC. 2020. Nat Immunol. 21: 362-364. PMID: 32205879
  10. A20 减轻小梁网的纤维化反应。  |  Mzyk, P., et al. 2022. Int J Mol Sci. 23: PMID: 35216043
  11. A20 和非经典 NF-κB 通路是胎儿发育过程中中性粒细胞招募的关键调节因子。  |  Rohwedder, I., et al. 2023. JCI Insight. 8: PMID: 36633909
  12. 与TNFAIP3（A20）单倍体不足相关的基因突变及其对炎症性疾病的影响。  |  Bagyinszky, E. and An, SSA. 2024. Int J Mol Sci. 25: PMID: 39125844

订购信息

产品名称产品编号规格价格数量收藏夹

A20/TNFAIP3 抗体 (A-12)

sc-166692
200 µg/ml
$316.00

A20/TNFAIP3 (A-12): m-IgG Fc BP-HRP 套装

sc-529032
200 µg Ab; 10 µg BP
$354.00

A20/TNFAIP3 (A-12): m-IgGκ BP-HRP 套装

sc-521713
200 µg Ab, 40 µg BP
$354.00

A20/TNFAIP3 (A-12): m-IgG2a BP-HRP 套装

sc-547240
200 µg Ab; 10 µg BP
$354.00

A20/TNFAIP3 抗体 (A-12) X

sc-166692 X
200 µg/0.1 ml
$316.00

A20/TNFAIP3 抗体 (A-12) AC

sc-166692 AC
500 µg/ml, 25% agarose
$416.00

A20/TNFAIP3 抗体 (A-12) HRP

sc-166692 HRP
200 µg/ml
$316.00

A20/TNFAIP3 抗体 (A-12) FITC

sc-166692 FITC
200 µg/ml
$330.00

A20/TNFAIP3 抗体 (A-12) PE

sc-166692 PE
200 µg/ml
$343.00

A20/TNFAIP3 抗体 (A-12) Alexa Fluor® 488

sc-166692 AF488
200 µg/ml
$357.00

A20/TNFAIP3 抗体 (A-12) Alexa Fluor® 546

sc-166692 AF546
200 µg/ml
$357.00

A20/TNFAIP3 抗体 (A-12) Alexa Fluor® 594

sc-166692 AF594
200 µg/ml
$357.00

A20/TNFAIP3 抗体 (A-12) Alexa Fluor® 647

sc-166692 AF647
200 µg/ml
$357.00

A20/TNFAIP3 抗体 (A-12) Alexa Fluor® 680

sc-166692 AF680
200 µg/ml
$357.00

A20/TNFAIP3 抗体 (A-12) Alexa Fluor® 790

sc-166692 AF790
200 µg/ml
$357.00

Hi, regarding the A20 ab with product number sc-16669 that's conjugated to AF647 (APC): what would be the price and size for 1 vial?

Asked by: JessicaV
Thank you for your question. Please contact your local distributor or our Technical Service team for pricing and size information of this antibody.
Answered by: Tech Service
Date published: 2019-02-14

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available conjugated to phycoerythrin (sc-166692 PE) or fluoroscein (sc-166692 FITC), Alexa Fluor® 488 (sc-166692 AF488) or Alexa Fluor® 647 (sc-166692 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 4 out of 5 by from Good for IFThis p38α/β Antibody (A-12), sc-7972, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 5 out of 5 by from Nice antibodyIts worked fine for me in 5% BSA in TBST to get god band
Date published: 2017-11-21
Rated 5 out of 5 by from 试用后评论我申请了试用装，工作人员非常热心，发货也快，用1:50-1:100的浓度做石蜡切片的免疫组化染色，染色效果还是很不错的。
Date published: 2017-10-02
Rated 4 out of 5 by from Clear band between 75 and 100 kDaClear band between 75 and 100 kDa was detected in western blotting. But when using 1:500 dilution, it is not very obvious.
Date published: 2017-09-29
Rated 4 out of 5 by from A20 antibodyI got this sample antibody a month ago.. It is great antibody Work for airway cells
Date published: 2017-09-22
Rated 5 out of 5 by from Good antibody.It works well for IF staining at dilution of 1:100.
Date published: 2017-07-24
Rated 4 out of 5 by from WBThis antibody was used to detect the expression of A20 by western blot. Positive signal was observed at the dilution of 1:500.sample ismouse
Date published: 2017-07-17
Rated 5 out of 5 by from Strong signalThe antibody used to analysis A20 in rat kidney tissue.I suggest use Tianneng image system for exposed untilthe signal appears very clearly at 90kda ,but not exposed too long,it may cause high background.
Date published: 2017-07-15
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A20/TNFAIP3 抗体 (A-12) is rated 4.7 out of 5 by 23.
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