Date published: 2026-10-17

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caspase-7 p20 抗体 (B-5): sc-28295

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说明书
  • caspase-7 p20抗体(B-5)是小鼠单克隆IgG1 κ, 在16篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的caspase-7 p20的N-terminus附近的氨基酸15-50之间
  • 抗-caspase-7 p20 抗体 (B-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 p20 subunit and precursor of caspase-7; 也和以下物种反应,包括: equine
  • 抗caspase-7 p20抗体(B-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是caspase-7 p20 Antibody (B-5) for WB and IHC(P) applications. 的首选辅助检测试剂,这些试剂现与caspase-7 p20 Antibody (B-5) 打包提供(请参阅下面的订购信息)。
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関連項目

caspase-7 p20抗体(B-5)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过应用诸如蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫 酶联免疫吸附试验(ELISA)。抗半胱天冬酶-7抗体(B-5)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。半胱氨酸蛋白酶-7是半胱氨酸蛋白酶-3/半胱氨酸蛋白酶-1家族的重要成员,该家族是细胞凋亡过程中不可或缺的,通过切割特定的靶蛋白来促进细胞凋亡,这些靶蛋白调节细胞的寿命。半胱氨酸蛋白酶-7的p20亚基在切割聚(ADP-核糖)聚合酶(PARP)中起作用,后者是一种参与DNA修复和细胞应激反应的核蛋白。Caspase-7能够将PARP切割成凋亡片段,这凸显了其在凋亡过程中的重要性,因为这一过程对于维持细胞稳态和防止受损细胞扩散至关重要。Caspase-7由上游caspase(如caspase-10)激活,以响应凋亡信号,这进一步凸显了其在细胞信号通路这一复杂网络中的作用,该网络控制着细胞的死亡和存活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-7 p20 抗体 (B-5) 参考文献:

  1. 细胞凋亡过程中自动修饰的聚(ADP-核糖)聚合酶的裂解。Caspase-7 参与其中的证据。  |  Germain, M., et al. 1999. J Biol Chem. 274: 28379-84. PMID: 10497198
  2. 以组成型活性 caspase-7 为诱饵进行酵母双杂交筛选,鉴定 PA28gamma 为效应 caspase 底物。  |  Araya, R., et al. 2002. Cell Death Differ. 9: 322-8. PMID: 11859414
  3. 人类癌症中 CASPASE-7 基因的失活突变。  |  Soung, YH., et al. 2003. Oncogene. 22: 8048-52. PMID: 12970753
  4. Caspase-7 基因干扰揭示了该酶在细胞凋亡早期阶段的参与情况。  |  Korfali, N., et al. 2004. J Biol Chem. 279: 1030-9. PMID: 14583630
  5. 参与细胞凋亡的人类基因 CPP32, Mch2, Mch3 和 Ich-1 的染色体定位。  |  Tiso, N., et al. 1996. Biochem Biophys Res Commun. 225: 983-9. PMID: 8780721
  6. Caspases:细胞凋亡的刽子手。  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844
  7. Fas 诱导小鼠肝脏细胞凋亡后,caspase-3 和 caspase-7 的亚细胞分布不同。  |  Chandler, JM., et al. 1998. J Biol Chem. 273: 10815-8. PMID: 9556551
  8. 前列腺癌细胞系 LNCaP 细胞凋亡过程中激活的信号通路:过表达 caspase-7 作为前列腺癌的一种新基因治疗策略。  |  Marcelli, M., et al. 1999. Cancer Res. 59: 382-90. PMID: 9927051

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-7 p20 抗体 (B-5)

sc-28295
200 µg/ml
$322.00

caspase-7 p20 (B-5): m-IgG Fc BP-HRP 套装

sc-526655
200 µg Ab; 10 µg BP
$361.00

caspase-7 p20 (B-5): m-IgG1 BP-HRP 套装

sc-532028
200 µg Ab; 20 µg BP
$361.00

caspase-7 p20 抗体 (B-5) AC

sc-28295 AC
500 µg/ml, 25% agarose
$424.00

caspase-7 p20 抗体 (B-5) HRP

sc-28295 HRP
200 µg/ml
$322.00

caspase-7 p20 抗体 (B-5) FITC

sc-28295 FITC
200 µg/ml
$336.00

caspase-7 p20 抗体 (B-5) PE

sc-28295 PE
200 µg/ml
$349.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 488

sc-28295 AF488
200 µg/ml
$364.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 546

sc-28295 AF546
200 µg/ml
$364.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 594

sc-28295 AF594
200 µg/ml
$364.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 647

sc-28295 AF647
200 µg/ml
$364.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 680

sc-28295 AF680
200 µg/ml
$364.00

caspase-7 p20 抗体 (B-5) Alexa Fluor® 790

sc-28295 AF790
200 µg/ml
$364.00

caspase-7 p20 (B-5) 中和勝肽

sc-28295 P
100 µg/0.5 ml
$69.00

What application is the blocking peptide sc-28295 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by sinlu from Western blotThis antibody work well in western blot (gastric cancer cell line: AGS, MKN45, dilute 1:1000)
Date published: 2017-11-14
Rated 5 out of 5 by MrHu from high sensitivityThe first thing to strike us was the amazing high specificity, sensitivity, convenience and quickness of the caspase-7 p20 antibody . It is really excellent.
Date published: 2017-10-18
Rated 5 out of 5 by alice2000 from target signalI used this antibody for WB analysis,I analysis the mouse colon tissue expression of the target protein,I got the target band and no addtional bands seen.
Date published: 2017-07-29
Rated 5 out of 5 by Meredith from importThe cysteine protease family is a specific target protein that is critical for the destruction of cell life. And we usually know that polymerase in DNA mutation and double-strand breaks in the measurement plays an indispensable role. This is our test in the oxygen-sliding process important test items
Date published: 2017-02-06
Rated 5 out of 5 by Dr Ninau Qelp from Amazing IHC(P) stainingcaspase-7 p20 (B-5) gave beautiful IHC(P) staining in human colon tissue showing cytoplasmic and nuclear staining of glandular cells. Also works really well for Western blot (WB).
Date published: 2017-02-03
Rated 5 out of 5 by MC from Good specificity for IHC(P) stainingGood specificity for IHC(P) staining. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by LF from Great detection of the p20 cleavage productGreat detection of the p20 cleavage product when HeLa or Jurkat are treated with UV or Staurosporine. -SCBT QC
Date published: 2014-11-12
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caspase-7 p20 抗体 (B-5) is rated 5.0 out of 5 by 7.
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