Smad4 Antibody (B-8): sc-7966
- Smad4 Antibody (B-8) is a mouse monoclonal IgG1 κ Smad4 antibody, cited in 823 publications, provided at 200 µg/ml
- raised against amino acids 1-552 representing full length Smad4 of human origin
- Smad4 Antibody (B-8) is recommended for detection of Smad4 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
- Anti-Smad4 Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to biotin for WB, IHC(P) and ELISA
- TransCruz reagent for Gel Supershift and ChIP applications (sc-7966 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Smad4 Antibody (B-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Smad4 Antibody (B-8) (see ordering information below).
Smad4 Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Smad4 of mouse, rat, and human origin through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Smad4 monoclonal antibody (B-8) is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. Smad4, also known as DPC4, plays a crucial role as a central mediator in the transforming growth factor-beta (TGFβ) and bone morphogenetic protein (BMP) signaling pathways, which are essential for regulating cellular processes such as proliferation, differentiation, and apoptosis. By interacting with other Smad family members, Smad4 facilitates transcriptional regulation of target genes, thereby influencing developmental processes and tissue homeostasis. Smad4 (B-8) antibody specificity allows researchers to effectively study function and interactions, particularly in the context of TGFβ/BMP signaling, where Smad4 is essential for mediating pathway effects. Additionally, Smad6 and Smad7 act as negative regulators of TGFβ signaling, highlighting Smad4 importance in maintaining the balance of these critical cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Smad4 Antibody (B-8) References:
- Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury. | Lin, AH., et al. 2005. J Immunol. 175: 547-54. PMID: 15972691
- Smads and chromatin modulation. | van Grunsven, LA., et al. 2005. Cytokine Growth Factor Rev. 16: 495-512. PMID: 15979924
- TGF-beta signalling through the Smad pathway. | Massaous, J. and Hata, A. 1997. Trends Cell Biol. 7: 187-92. PMID: 17708943
- A human Mad protein acting as a BMP-regulated transcriptional activator. | Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
- MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. | Eppert, K., et al. 1996. Cell. 86: 543-52. PMID: 8752209
- Receptor-associated Mad homologues synergize as effectors of the TGF-beta response. | Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
- Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways. | Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
- Smad6 inhibits signalling by the TGF-beta superfamily. | Imamura, T., et al. 1997. Nature. 389: 622-6. PMID: 9335505
- Smad8 mediates the signaling of the ALK-2 [corrected] receptor serine kinase. | Chen, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12938-43. PMID: 9371779
- TGF-beta signalling from cell membrane to nucleus through SMAD proteins. | Heldin, CH., et al. 1997. Nature. 390: 465-71. PMID: 9393997
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Smad4 Antibody (B-8) | sc-7966 | 200 µg/ml | $322.00 | |||
Smad4 Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-526505 | 200 µg Ab; 10 µg BP | $361.00 | |||
Smad4 Antibody (B-8): m-IgG1 BP-HRP Bundle | sc-531878 | 200 µg Ab; 20 µg BP | $361.00 | |||
Smad4 Antibody (B-8) X | sc-7966 X | 200 µg/0.1 ml | $322.00 | |||
Smad4 Antibody (B-8) AC | sc-7966 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Smad4 Antibody (B-8) HRP | sc-7966 HRP | 200 µg/ml | $322.00 | |||
Smad4 Antibody (B-8) FITC | sc-7966 FITC | 200 µg/ml | $336.00 | |||
Smad4 Antibody (B-8) PE | sc-7966 PE | 200 µg/ml | $349.00 | |||
Smad4 Antibody (B-8) Alexa Fluor® 488 | sc-7966 AF488 | 200 µg/ml | $364.00 | |||
Smad4 Antibody (B-8) Alexa Fluor® 546 | sc-7966 AF546 | 200 µg/ml | $364.00 | |||
Smad4 Antibody (B-8) Alexa Fluor® 594 | sc-7966 AF594 | 200 µg/ml | $364.00 | |||
Smad4 Antibody (B-8) Alexa Fluor® 647 | sc-7966 AF647 | 200 µg/ml | $364.00 | |||
Smad4 Antibody (B-8) Alexa Fluor® 680 | sc-7966 AF680 | 200 µg/ml | $364.00 | |||
Smad4 Antibody (B-8) Alexa Fluor® 790 | sc-7966 AF790 | 200 µg/ml | $364.00 | |||
Smad4 Antibody (B-8) B | sc-7966 B | 200 µg/ml | $326.00 |