Date published: 2025-9-8
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Histone H3 Anticorpo (1G1): sc-517576

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Fichas de dados
  • Histone H3 Anticorpo 1G1é um anticorpo monoclonal produzido em camundongo IgG, citado em 87 publicações, fornecido em 100 µg/ml
  • sensibilizados contra fragmentos de proteínas Histone H3 recombinantes de origem human
  • recomendado para a detecção de Histone H3 de mouse, rat e human origem em
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de Histone H3 (1G1): sc-517576.
  • m-IgG Fc BP-HRP e 1 BP-HRP">m-IgG1 BP-HRP são os reagentes de deteção secundários preferidos para Histone H3 Antibody (1G1) for WB applications. Estes reagentes são agora oferecidos em conjuntos com Histone H3 Antibody (1G1)(ver informações de encomenda abaixo).

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Produtos auxiliares
Descricao
Identificacao do gene
Ficha de dados e Protocolos
Informações de investigação

VEJA TAMBÉM

Adicional Histone Anticorpos incluindo Histone cluster 1 H1, Histone H2A, Histone H2B, Histone H3, Histone H4 e Histone cluster 1 H3A

O anticorpo anti-Histona H3 (1G1) é um anticorpo IgG monoclonal de ratinho que detecta a Histona H3 em amostras de camundongo, rato e humano por western blotting (WB) e imunofluorescência (IF). O anticorpo anti-Histona H3 (1G1) está disponível na forma não conjugada. A histona H3 desempenha um papel crucial na organização estrutural da cromatina, essencial para o empacotamento do ADN no núcleo. A histona H3 forma uma parte central da estrutura octamérica do nucleossoma que envolve o ADN, regulando a expressão genética e a replicação do ADN. A histona H3 mantém a estabilidade genómica e facilita os processos de transcrição e reparação. A histona H3 sofre várias modificações pós-traducionais, incluindo metilação e acetilação, que influenciam as interações entre o ADN e as proteínas, afectando a estrutura e a função da cromatina. Estas modificações regulam a expressão genética e têm impacto na diferenciação celular e na resposta ambiental. As capacidades de deteção do anticorpo anti-Histona H3 (1G1) tornam este reagente valioso para o estudo da dinâmica da cromatina e da regulação epigenética em vários contextos biológicos.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

Histone H3 Anticorpo (1G1)

sc-517576
100 µg/ml
$316.00

Pacote do Histone H3 (1G1): m-IgG Fc BP-HRP

sc-541115
100 µg Ab; 10 µg BP
$354.00

Pacote do Histone H3 (1G1): m-IgG1 BP-HRP

sc-542591
100 µg Ab; 20 µg BP
$354.00

I performed ICC with this antibody in mitotic cells, but I couldn't detect histone H3 signal co-localized with the DAPI signal. Could you help me solve this issue?

Asked by: KWJ94
Thank you for your product review. We are sorry to hear that this antibody did not perform for you as expected. There are many possible causes for no signal. Please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website, and we would be happy to help you figure this out. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase and will do our best to help you achieve the best results possible!
Answered by: Jiajun Z
Date published: 2024-09-29

Hi can we please get a sample of sc-517576? Do you have that available? We just purchased sc-390935 with Texas A&M University Biology Department, Dr. Deb Bell-Pedersen lab.

Asked by: Johnny
Thank you for contacting Santa Cruz Biotechnology. To request a sample please email scbt@scbt.com
Answered by: BlakeJ
Date published: 2024-06-18

Just received a free sample, which dilution would you recommend for nuclear extracts WB?

Asked by: IngOlmos
Thank you for your question. Dilution recommendations can be found in the product's datasheet, which is available right here on the product page. For western blots we recommend a starting dilution of 1:200, and a dilution range between 1:100-1:1000. When working with nuclear extracts, an amount of protein between 10–20 µg is appropriate.
Answered by: Tech Support Europe
Date published: 2023-05-15

could it be possible to buy directly bound to alexa488 or 594 please

Asked by: antonio blesa
Thank you for your question. If you are interested in this antibody directly conjugated with AF488 or AF594, please contact our our Technical Service. We should be able to provide this format as special order.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-31
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Rated 4 out of 5 by from Good work!!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2023-02-17
Rated 5 out of 5 by from H3 Ab for Western BlottingAsked for a sample before purchasing. Worked straight away on CRC cell lines HCT116 and DLD1 (drug treated and untreated) when loading 20 ug of whole protein lysates and using 1:500 dilution in 5% BSA/TBST. Would definitely recommend.
Date published: 2023-01-24
Rated 5 out of 5 by from western blotI used this antibody for my mouse primary neuron cells western blot. I got very nice and clean bolt!
Date published: 2022-08-24
Rated 5 out of 5 by from good and strongThe antibody was tested on whole lysate from liver cancer cell lines (JHH6 andHuh7) and immortalized hepatocytes (IHH). Antibody: sensitive and specific. Too strong signal with the dilution you recommend. Conditions need to be better set. Apparent MW: about 15kDa. Expected MW: 15kDa. • 40ug/lane cell lysate • Transfer 1h30 100V, PVDF (0.2 µm) • Blocking milk 4% in 0.1% Tween -PBS 1h RT • Anti-HISTONE H3 (1G1)(sc-517576) 1:200 O/N 4°C in blocking solution • Anti-mouse HRP 1:1000 1h RT in blocking solution • Developed with ECL Plus
Date published: 2022-06-09
Rated 5 out of 5 by from Very good!In MB231 cells, a clear band appeared after WB analysis 1: 2000 dilution.
Date published: 2022-03-18
Rated 4 out of 5 by from Useful for my purposesI asked it as free trial and i worked well enough, so I think I can use it for my purposes.
Date published: 2020-12-23
Rated 5 out of 5 by from Great for IFBought this antibody recently for the purpose of immunofluorescence on human neuroblastoma cell lines. It worked very well.
Date published: 2019-09-03
Rated 5 out of 5 by from Works very well with 1:5000 dilutionWorks very well with 1:5000 dilution, and I hope some more conjugation products can be developed with this antibody.
Date published: 2019-02-05
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Histone H3 Anticorpo (1G1) is rated 4.7 out of 5 by 10.
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