Ac-Histone H3 Antibody (AH3-120): sc-56616

Ac-Histone H3 Antibody (AH3-120) is a mouse monoclonal IgG1 kappa light chain antibody raised against an amino acid sequence containing acetylated Lysine 9 of Histone H3 of human origin, and reacts with proteins from mouse, rat, human, Drosophila, Xenopus, C. elegans, and bovine species; anti-Ac-Histone H3 antibody (AH3-120) is suitable for applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Histone H3 is a core component of the nucleosome, the fundamental unit of chromatin in eukaryotic cells, where DNA is wrapped around histone octamers composed of Histones H2A, H2B, H3, and H4, resulting in the dense packing of DNA that regulates access to genetic information. The acetylation of Histone H3 at lysine 9 is a critical post-translational modification that plays a significant role in gene regulation by altering chromatin structure; this modification reduces the positive charge on histone tails, weakening their interaction with negatively charged DNA and leading to a more relaxed chromatin state that facilitates the binding of transcription factors and the initiation of gene transcription. Understanding the function of acetylated Histone H3 at lysine 9 is important because this modification is associated with active gene expression and is involved in various biological processes such as cell cycle progression, differentiation, and response to environmental stimuli; dysregulation of this modification has been implicated in diseases like cancer and neurodegenerative disorders. Ac-Histone H3 (AH3-120) monoclonal antibody provides a valuable tool for researchers studying epigenetic mechanisms, chromatin dynamics, and the regulation of gene expression, contributing to a deeper understanding of cellular function and the development of therapeutic strategies.

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Ac-Histone H3 Antibody (AH3-120) References:

1. Antibodies to defined histone epitopes reveal variations in chromatin conformation and underacetylation of centric heterochromatin in human metaphase chromosomes.  |  Jeppesen, P., et al. 1992. Chromosoma. 101: 322-32. PMID: 1374304
2. The H1 and core histone subtypes: differential gene expression and varied primary structures.  |  Doenecke, D., et al. 1988. Adv Enzyme Regul. 27: 107-20. PMID: 3250226
3. Analysis of nucleosome assembly and histone exchange using antibodies specific for acetylated H4.  |  Perry, CA., et al. 1993. Biochemistry. 32: 13605-14. PMID: 7504954
4. Temporally restricted spatial localization of acetylated isoforms of histone H4 and RNA polymerase II in the 2-cell mouse embryo.  |  Worrad, DM., et al. 1995. Development. 121: 2949-59. PMID: 7555721
5. Histone H1.  |  Wolffe, AP. 1997. Int J Biochem Cell Biol. 29: 1463-6. PMID: 9570139
6. Overlapping but distinct patterns of histone acetylation by the human coactivators p300 and PCAF within nucleosomal substrates.  |  Schiltz, RL., et al. 1999. J Biol Chem. 274: 1189-92. PMID: 9880483

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ac-Histone H3 Antibody (AH3-120) has 42 citations in a variety of scientific publications.

Primary antibodies like Anti-Ac-Histone H3 Antibody (AH3-120) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ac-Histone H3 Antibody (AH3-120) Specifications

Alternate Product Name: acetyl Histone H3

Product Clone Name: AH3-120

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Histone H3

Epitope Species: human

Product Reactivity/Detection: Ac-Histone H3

Species Reactivity/Detection: mouse, rat, human, Drosophila, Xenopus, C. elegans, bovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 42 citations in various scientific publications

Available Conjugates/Forms for Ac-Histone H3 Antibody (AH3-120): N/A