Ac-Histone H3 Antibody (D-4): sc-518011

Ac-Histone H3 Antibody (D-4) is a mouse monoclonal IgG3 kappa light chain antibody that detects Ac-Histone H3, acetylated at Lys 9 and Lys 14, in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ac-Histone H3 antibody (D-4) is available in a non-conjugated form, allowing for versatile experimental design. Histone H3 plays a crucial role in regulating gene expression as a key component of the nucleosome, the fundamental unit of chromatin structure. Acetylation of Histone H3 at specific lysine residues, particularly Lys 9 and Lys 14, represents a significant post-translational modification that influences chromatin dynamics and accessibility, thereby impacting transcriptional activity. This modification is primarily mediated by histone acetyltransferases such as p300 and PCAF, which facilitate chromatin structure loosening, allowing transcription factors and other regulatory proteins to access DNA more readily. The dynamic interplay of acetylated histones with other histone proteins suggests a complex regulatory mechanism essential for proper gene expression and cellular function.

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Ac-Histone H3 Antibody (D-4) References:

1. Differentiation between peptides containing acetylated or tri-methylated lysines by mass spectrometry: an application for determining lysine 9 acetylation and methylation of histone H3.  |  Zhang, K., et al. 2004. Proteomics. 4: 1-10. PMID: 14730666
2. Expression of acetylated and dimethylated histone H3 in colorectal cancer.  |  Hashimoto, T., et al. 2013. Dig Surg. 30: 249-58. PMID: 23921187
3. Association of Taf14 with acetylated histone H3 directs gene transcription and the DNA damage response.  |  Shanle, EK., et al. 2015. Genes Dev. 29: 1795-800. PMID: 26341557
4. Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue.  |  Janczura, KJ., et al. 2018. J Vis Exp.. PMID: 30582583
5. Acetylated histone H4 tail enhances histone H3 tail acetylation by altering their mutual dynamics in the nucleosome.  |  Furukawa, A., et al. 2020. Proc Natl Acad Sci U S A. 117: 19661-19663. PMID: 32747537
6. Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.  |  Yang, G., et al. 2021. EMBO Rep. 22: e50967. PMID: 33372411
7. Methylation-directed acetylation of histone H3 regulates developmental sensitivity to histone deacetylase inhibition.  |  Huang, LY., et al. 2021. Nucleic Acids Res. 49: 3781-3795. PMID: 33721015
8. Nuclear localization of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation.  |  Li, W., et al. 2022. Nat Commun. 13: 7414. PMID: 36460681
9. Epigenetic memory in breast cancer: electrochemical biosensor-based tracing of histone H3 acetylation inheritance.  |  Youshanlui, MA. and Shirvaliloo, M. 2023. Epigenomics. 15: 111-114. PMID: 37020410
10. Acetylation of Histone H3 in Cancer Progression and Prognosis.  |  Miziak, P., et al. 2024. Int J Mol Sci. 25: PMID: 39456765

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ac-Histone H3 Antibody (D-4) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Ac-Histone H3 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ac-Histone H3 Antibody (D-4) Specifications

Alternate Product Name: acetyl Histone H3

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 1-28

Epitope Target: Histone H3

Epitope Species: human

Product Reactivity/Detection: Histone H3 acetylated at Lys 9 and Lys 14

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Ac-Histone H3 Antibody (D-4): N/A

The preferred Ac-Histone H3 antibody is Ac-Histone H3 Antibody (AH3-120): sc-56616.