ZO-1 Antibody (R40.76): sc-33725

ZO-1 Antibody (R40.76) is a rat monoclonal IgG2a antibody designed to detect the Zonula Occludens-1 protein, also known as Tight Junction Protein 1 (TJP1), in mouse, rat, and human samples using western blotting (WB), immunoprecipitation (IP), and PAR assays. Anti-ZO-1 antibody (R40.76) is raised against DOC-insoluble junctional ribbons isolated from rat liver, ensuring reliable recognition of the ZO-1 epitope. ZO-1 is a critical component of tight junctions, which are essential for maintaining the integrity and selective permeability of epithelial and endothelial cell layers. As a member of the membrane-associated guanylate kinase (MAGUK) family, ZO-1 interacts with other tight junction proteins such as occludin, claudins, ZO-2, and ZO-3 through its PDZ, SH3, and guanylate kinase domains, facilitating both structural stability and signal transduction. These interactions link the tight junction complexes to the actin cytoskeleton, playing a vital role in regulating cell polarity, barrier function, and cellular communication. Dysregulation of ZO-1 has been associated with various pathological conditions, including cancer metastasis, where ZO-1 expression is notably reduced in many breast cancer cell lines, as well as in cardiovascular and neurological disorders. Anti-ZO-1 antibody (R40.76) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates, providing versatile options for different experimental applications. ZO-1 monoclonal antibody (R40.76) enables researchers to investigate the expression, localization, and functional dynamics of ZO-1, thereby advancing our understanding of cellular junctions and their role in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ZO-1 Antibody (R40.76) References:

1. Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin.  |  Itoh, M., et al. 1999. J Biol Chem. 274: 5981-6. PMID: 10026224
2. Manner of interaction of heterogeneous claudin species within and between tight junction strands.  |  Furuse, M., et al. 1999. J Cell Biol. 147: 891-903. PMID: 10562289
3. Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3.  |  Wittchen, ES., et al. 1999. J Biol Chem. 274: 35179-85. PMID: 10575001
4. Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins.  |  Itoh, M., et al. 1999. J Cell Biol. 147: 1351-63. PMID: 10601346
5. Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions.  |  Furuse, M., et al. 1994. J Cell Biol. 127: 1617-26. PMID: 7798316
6. Cell signalling: MAGUK magic.  |  Anderson, JM. 1996. Curr Biol. 6: 382-4. PMID: 8723338
7. ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin.  |  Haskins, J., et al. 1998. J Cell Biol. 141: 199-208. PMID: 9531559
8. Loss of the tight junction MAGUK ZO-1 in breast cancer: relationship to glandular differentiation and loss of heterozygosity.  |  Hoover, KB., et al. 1998. Am J Pathol. 153: 1767-73. PMID: 9846967

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZO-1 Antibody (R40.76) has 218 citations in a variety of scientific publications.

Primary antibodies like Anti-ZO-1 Antibody (R40.76) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZO-1 Antibody (R40.76) Specifications

Alternate Product Name: ZO-1

Product Clone Name: R40.76

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: ZO-1

Epitope Species: rat

Product Reactivity/Detection: ZO-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 218 citations in various scientific publications

Available Conjugates/Forms for ZO-1 Antibody (R40.76): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin