ZnT-8 Antibody (B-9): sc-514715

ZnT-8 Antibody (B-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects ZnT-8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZnT-8 (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZnT-8, also known as SLC30A8, is a critical component in regulating zinc homeostasis within pancreatic islet cells, functioning as a zinc-efflux transporter. ZnT-8 is essential for proper insulin maturation, facilitating zinc accumulation in intracellular vesicles, which is vital for insulin crystal formation. ZnT-8′s importance extends beyond insulin processing as a major autoantigen in type I diabetes, making ZnT-8 a significant target for autoimmune response research. Additionally, ZnT-8′s structure, characterized by a multi-pass membrane configuration and six membrane-spanning domains, allows effective zinc ion transport across cellular membranes, influencing various cellular functions and metabolic processes. Multiple ZnT-8 isoforms arise from alternative splicing, further underscoring ZnT-8′s complexity and regulatory potential in health and disease.

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ZnT-8 Antibody (B-9) References:

1. In silico identification and expression of SLC30 family genes: an expressed sequence tag data mining strategy for the characterization of zinc transporters' tissue expression.  |  Seve, M., et al. 2004. BMC Genomics. 5: 32. PMID: 15154973
2. Identification and cloning of a beta-cell-specific zinc transporter, ZnT-8, localized into insulin secretory granules.  |  Chimienti, F., et al. 2004. Diabetes. 53: 2330-7. PMID: 15331542
3. ZnT-8, a pancreatic beta-cell-specific zinc transporter.  |  Chimienti, F., et al. 2005. Biometals. 18: 313-7. PMID: 16158222
4. Mutations in the SLC30A8 gene are not a major cause of MODY or other forms of early-onset, autosomal dominant type 2 diabetes.  |  Borowiec, M., et al. 2007. Diabetologia. 50: 2224-6. PMID: 17657472
5. The cation efflux transporter ZnT8 (Slc30A8) is a major autoantigen in human type 1 diabetes.  |  Wenzlau, JM., et al. 2007. Proc Natl Acad Sci U S A. 104: 17040-5. PMID: 17942684
6. Intracellular zinc homeostasis in leukocyte subsets is regulated by different expression of zinc exporters ZnT-1 to ZnT-9.  |  Overbeck, S., et al. 2008. J Leukoc Biol. 83: 368-80. PMID: 17971500
7. A polymorphism in the zinc transporter gene SLC30A8 confers resistance against posttransplantation diabetes mellitus in renal allograft recipients.  |  Kang, ES., et al. 2008. Diabetes. 57: 1043-7. PMID: 18162509
8. A common nonsynonymous single nucleotide polymorphism in the SLC30A8 gene determines ZnT8 autoantibody specificity in type 1 diabetes.  |  Wenzlau, JM., et al. 2008. Diabetes. 57: 2693-7. PMID: 18591387
9. Three-dimensional structure of beta-cell-specific zinc transporter, ZnT-8, predicted from the type 2 diabetes-associated gene variant SLC30A8 R325W.  |  Weijers, RN. 2010. Diabetol Metab Syndr. 2: 33. PMID: 20525392
10. SLC30A8 mutations in type 2 diabetes.  |  Rutter, GA. and Chimienti, F. 2015. Diabetologia. 58: 31-6. PMID: 25287711
11. Genetic, Epigenetic and Biological Effects of Zinc Transporter (SLC30A8) in Type 1 and Type 2 Diabetes.  |  Gu, HF. 2017. Curr Diabetes Rev. 13: 132-140. PMID: 26593983
12. Multiple genetic variants at the SLC30A8 locus affect local super-enhancer activity and influence pancreatic β-cell survival and function.  |  Hu, M., et al. 2024. FASEB J. 38: e23610. PMID: 38661000

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZnT-8 Antibody (B-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ZnT-8 Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZnT-8 Antibody (B-9) Specifications

Alternate Product Name: ZnT-8

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-79

Epitope Target: ZnT-8

Epitope Species: mouse

Product Reactivity/Detection: ZnT-8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ZnT-8 Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin