ZNF74 Antibody (B-11): sc-390612
- ZNF74 Antibody (B-11) is a mouse monoclonal IgG1 κ ZNF74 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 621-639 at the C-terminus of ZNF74 of human origin
- ZNF74 Antibody (B-11) is recommended for detection of ZNF74 of human origin by WB, IP, IF and ELISA
- Anti-ZNF74 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-390612 X, 200 µg/0.1 ml)
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ZNF74 Antibody (B-11) for WB applications. These reagents are now offered in bundles with ZNF74 Antibody (B-11) (see ordering information below).
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ZNF74 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZNF74 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-ZNF74 antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZNF74, a member of the Krüppel C2H2-type zinc-finger protein family, plays a crucial role in gene regulation due to its ability to bind DNA through zinc-finger motifs. Specifically, ZNF74 contains one KRAB domain and twelve C2H2-type zinc fingers, which facilitate interaction with the nuclear matrix and enable engagement in protein-protein interactions essential for transcriptional regulation. ZNF74′s importance is underscored by involvement in DiGeorge syndrome, where deletions of the gene encoding ZNF74 on chromosome 22 are linked to various developmental issues, including congenital heart defects and learning disabilities. Additionally, ZNF74 undergoes alternative splicing, resulting in four distinct isoforms, which may contribute to functional diversity and regulatory mechanisms within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZNF74 Antibody (B-11) References:
- ZNF74, a gene deleted in DiGeorge syndrome, is expressed in human neural crest-derived tissues and foregut endoderm epithelia. | Ravassard, P., et al. 1999. Genomics. 62: 82-5. PMID: 10585771
- Alternative promoter usage and splicing of ZNF74 multifinger gene produce protein isoforms with a different repressor activity and nuclear partitioning. | Côté, F., et al. 2001. DNA Cell Biol. 20: 159-73. PMID: 11313019
- Association of ZNF74 gene genotypes with age-at-onset of schizophrenia. | Takase, K., et al. 2001. Schizophr Res. 52: 161-5. PMID: 11705709
- Mutation screening and LD mapping in the VCFS deleted region of chromosome 22q11 in schizophrenia using a novel DNA pooling approach. | Williams, NM., et al. 2002. Mol Psychiatry. 7: 1092-100. PMID: 12476324
- Multifunctional zinc finger proteins in development and disease. | Ladomery, M. and Dellaire, G. 2002. Ann Hum Genet. 66: 331-42. PMID: 12485467
- Oligomerization of transcriptional intermediary factor 1 regulators and interaction with ZNF74 nuclear matrix protein revealed by bioluminescence resonance energy transfer in living cells. | Germain-Desprez, D., et al. 2003. J Biol Chem. 278: 22367-73. PMID: 12684500
- Gene expression microarray analysis and genome databases facilitate the characterization of a chromosome 22 derived homogeneously staining region. | Arcand, SL., et al. 2004. Mol Carcinog. 41: 17-38. PMID: 15352123
- Incidences of micro-deletion/duplication 22q11.2 detected by multiplex ligation-dependent probe amplification in patients with congenital cardiac disease who are scheduled for cardiac surgery. | Hu, Y., et al. 2009. Cardiol Young. 19: 179-84. PMID: 19224675
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF74 Antibody (B-11) | sc-390612 | 200 µg/ml | $322.00 | |||
ZNF74 Antibody (B-11): m-IgGκ BP-HRP Bundle | sc-523604 | 200 µg Ab, 40 µg BP | $361.00 | |||
ZNF74 Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-533597 | 200 µg Ab; 20 µg BP | $361.00 | |||
ZNF74 Antibody (B-11) X | sc-390612 X | 200 µg/0.1 ml | $322.00 | |||
ZNF74 Antibody (B-11) AC | sc-390612 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ZNF74 Antibody (B-11) HRP | sc-390612 HRP | 200 µg/ml | $322.00 | |||
ZNF74 Antibody (B-11) FITC | sc-390612 FITC | 200 µg/ml | $336.00 | |||
ZNF74 Antibody (B-11) PE | sc-390612 PE | 200 µg/ml | $349.00 | |||
ZNF74 Antibody (B-11) Alexa Fluor® 488 | sc-390612 AF488 | 200 µg/ml | $364.00 | |||
ZNF74 Antibody (B-11) Alexa Fluor® 546 | sc-390612 AF546 | 200 µg/ml | $364.00 | |||
ZNF74 Antibody (B-11) Alexa Fluor® 594 | sc-390612 AF594 | 200 µg/ml | $364.00 | |||
ZNF74 Antibody (B-11) Alexa Fluor® 647 | sc-390612 AF647 | 200 µg/ml | $364.00 | |||
ZNF74 Antibody (B-11) Alexa Fluor® 680 | sc-390612 AF680 | 200 µg/ml | $364.00 | |||
ZNF74 Antibody (B-11) Alexa Fluor® 790 | sc-390612 AF790 | 200 µg/ml | $364.00 | |||
ZNF74 (B-11) Neutralizing Peptide | sc-390612 P | 100 µg/0.5 ml | $69.00 |