ZNF281 Antibody (D-8): sc-166933

ZNF281 (D-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects ZNF281 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). ZNF281 (D-8) antibody is available as both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF281, also known as GC-box-binding zinc finger protein 1, ZBP-99 or ZNP-99 (zinc finger DNA-binding protein 99), is a zinc finger protein that belongs to the Krüppel C2H2-type zinc finger protein family. ZNF281 is expressed ubiquitously at low levels with predominant expression in kidney, liver, lymphocytes and placenta. ZNF281 localizes to the nucleus and contains four C2H2-type zinc fingers. ZNF281 plays a role in repressing the transcription of various genes including Gastrin and ODC (ornithine decarboxylase). ZNF281 functions by binding to the G-rich box in the enhancer region of the gene. Upon DNA damage, ZNF281 may become phosphorylated by Atm or ATR.

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ZNF281 Antibody (D-8) References:

1. Identification of human GC-box-binding zinc finger protein, a new Krüppel-like zinc finger protein, by the yeast one-hybrid screening with a GC-rich target sequence.  |  Lisowsky, T., et al. 1999. FEBS Lett. 453: 369-74. PMID: 10405178
2. ZBP-99 defines a conserved family of transcription factors and regulates ornithine decarboxylase gene expression.  |  Law, DJ., et al. 1999. Biochem Biophys Res Commun. 262: 113-20. PMID: 10448078
3. Hox proteins functionally cooperate with the GC box-binding protein system through distinct domains.  |  Suzuki, M., et al. 2003. J Biol Chem. 278: 30148-56. PMID: 12771139
4. ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1.  |  Zhang, X., et al. 2003. Nucleic Acids Res. 31: 2900-14. PMID: 12771217
5. Genomic gains on chromosome 1q in retinoblastoma: consequences on gene expression and association with clinical manifestation.  |  Gratias, S., et al. 2005. Int J Cancer. 116: 555-63. PMID: 15825178
6. Analysis of promoter regions of co-expressed genes identified by microarray analysis.  |  Veerla, S. and Höglund, M. 2006. BMC Bioinformatics. 7: 384. PMID: 16916454
7. Large-scale identification of c-MYC-associated proteins using a combined TAP/MudPIT approach.  |  Koch, HB., et al. 2007. Cell Cycle. 6: 205-17. PMID: 17314511
8. One hit, two hits, three hits, more? Genomic changes in the development of retinoblastoma.  |  Corson, TW. and Gallie, BL. 2007. Genes Chromosomes Cancer. 46: 617-34. PMID: 17437278
9. ZNF281 Promotes Growth and Invasion of Pancreatic Cancer Cells by Activating Wnt/β-Catenin Signaling.  |  Qian, Y., et al. 2017. Dig Dis Sci. 62: 2011-2020. PMID: 28523575
10. ZNF281 inhibits neuronal differentiation and is a prognostic marker for neuroblastoma.  |  Pieraccioli, M., et al. 2018. Proc Natl Acad Sci U S A. 115: 7356-7361. PMID: 29941555
11. ZNF281 is recruited on DNA breaks to facilitate DNA repair by non-homologous end joining.  |  Nicolai, S., et al. 2020. Oncogene. 39: 754-766. PMID: 31570788
12. Inhibition of Annexin A10 Contributes to ZNF281 Mediated Aggressiveness of Hepatocellular Carcinoma.  |  Zhang, X., et al. 2023. J Hepatocell Carcinoma. 10: 553-571. PMID: 37041757

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZNF281 Antibody (D-8) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-ZNF281 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZNF281 Antibody (D-8) Specifications

Alternate Product Name: ZNF281

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 679-895

Epitope Target: ZNF281

Epitope Species: human

Product Reactivity/Detection: ZNF281

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for ZNF281 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent