ZHX1 Antibody (E-6): sc-514284
- ZHX1 Antibody (E-6) is a mouse monoclonal IgG2b κ ZHX1 antibody provided at 200 µg/ml
- raised against amino acids 771-873 mapping at the C-terminus of ZHX1 of human origin
- ZHX1 Antibody (E-6) is recommended for detection of ZHX1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ZHX1 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ZHX1 (E-6): sc-514284.
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ZHX1 Antibody (E-6) for WB applications. These reagents are now offered in bundles with ZHX1 Antibody (E-6) (see ordering information below).
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ZHX1 Antibody (E-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects ZHX1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZHX1 (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZHX1 functions as a transcriptional repressor, primarily localized in the nucleus, where ZHX1 regulates gene expression by interacting with the activation domain of the A subunit of nuclear factor-Y (NF-YA). This interaction maintains proper cellular function and development, as dysregulation of ZHX1 can lead to various diseases, including hepatocellular carcinoma, where hypermethylation-mediated silencing of ZHX2 has been implicated. ZHX proteins, including ZHX1, feature two Cys(2)-His(2)-type zinc-finger motifs and five homeodomains, allowing formation of homodimers and heterodimers, although dimerization is not essential for repressor activity. This structural complexity highlights ZHX1′s role in transcriptional regulation and potential as a target for therapeutic intervention in diseases associated with aberrant gene expression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZHX1 Antibody (E-6) References:
- Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y. | Yamada, K., et al. 1999. Biochem Biophys Res Commun. 261: 614-21. PMID: 10441475
- Rat zinc-fingers and homeoboxes 1 (ZHX1), a nuclear factor-YA-interacting nuclear protein, forms a homodimer. | Hirano, S., et al. 2002. Gene. 290: 107-14. PMID: 12062805
- Functional analysis and the molecular dissection of zinc-fingers and homeoboxes 1 (ZHX1). | Yamada, K., et al. 2002. Biochem Biophys Res Commun. 297: 368-74. PMID: 12237128
- Genomic structure and analysis of transcriptional regulation of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene. | Shou, Z., et al. 2003. Gene. 302: 83-94. PMID: 12527199
- Analysis of zinc-fingers and homeoboxes (ZHX)-1-interacting proteins: molecular cloning and characterization of a member of the ZHX family, ZHX3. | Yamada, K., et al. 2003. Biochem J. 373: 167-78. PMID: 12659632
- Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor. | Kawata, H., et al. 2003. Biochem J. 373: 747-57. PMID: 12741956
- The mouse zinc-fingers and homeoboxes (ZHX) family; ZHX2 forms a heterodimer with ZHX3. | Kawata, H., et al. 2003. Gene. 323: 133-40. PMID: 14659886
- Promoter hypermethylation of a novel gene, ZHX2, in hepatocellular carcinoma. | Lv, Z., et al. 2006. Am J Clin Pathol. 125: 740-6. PMID: 16707376
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZHX1 Antibody (E-6) | sc-514284 | 200 µg/ml | $316.00 | |||
ZHX1 Antibody (E-6): m-IgGκ BP-HRP Bundle | sc-524671 | 200 µg Ab, 40 µg BP | $354.00 | |||
ZHX1 Antibody (E-6): m-IgG2b BP-HRP Bundle | sc-549173 | 200 µg Ab; 10 µg BP | $354.00 | |||
ZHX1 Antibody (E-6) AC | sc-514284 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ZHX1 Antibody (E-6) HRP | sc-514284 HRP | 200 µg/ml | $316.00 | |||
ZHX1 Antibody (E-6) FITC | sc-514284 FITC | 200 µg/ml | $330.00 | |||
ZHX1 Antibody (E-6) PE | sc-514284 PE | 200 µg/ml | $343.00 | |||
ZHX1 Antibody (E-6) Alexa Fluor® 488 | sc-514284 AF488 | 200 µg/ml | $357.00 | |||
ZHX1 Antibody (E-6) Alexa Fluor® 546 | sc-514284 AF546 | 200 µg/ml | $357.00 | |||
ZHX1 Antibody (E-6) Alexa Fluor® 594 | sc-514284 AF594 | 200 µg/ml | $357.00 | |||
ZHX1 Antibody (E-6) Alexa Fluor® 647 | sc-514284 AF647 | 200 µg/ml | $357.00 | |||
ZHX1 Antibody (E-6) Alexa Fluor® 680 | sc-514284 AF680 | 200 µg/ml | $357.00 | |||
ZHX1 Antibody (E-6) Alexa Fluor® 790 | sc-514284 AF790 | 200 µg/ml | $357.00 |