ZFR Antibody (B-8): sc-365743
- ZFR Antibody (B-8) is a mouse monoclonal IgG1 κ ZFR antibody provided at 200 µg/ml
- raised against amino acids 418-570 mapping within an internal region of ZFR of human origin
- ZFR Antibody (B-8) is recommended for detection of ZFR of human origin by WB, IP, IF and ELISA
- Anti-ZFR Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-365743 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ZFR Antibody (B-8) for WB applications. These reagents are now offered in bundles with ZFR Antibody (B-8) (see ordering information below).
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SEE ALSO...
ZFR Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZFR of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZFR (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZFR, also known as zinc finger RNA-binding protein or M-phase phosphoprotein homolog, is a crucial 1,074 amino acid RNA-binding protein that plays a significant role in regulating postimplantation and gastrulation stages of development. ZFR is predominantly expressed in human tissues such as the lung, liver, lymphocytes, heart, pancreas, kidney, and placenta, while murine ZFR is found in the testis, ovary, and brain. Notably, ZFR contains a DZF domain and is localized in the somatodendritic compartment of hippocampal neurons, where ZFR colocalizes with STAU2 in cytosolic RNA granules. ZFR interaction with specific proteins is vital for function; for instance, ZFR associates with chromosome foci during meiosis, highlighting its importance in gametogenesis. Furthermore, knockout studies in mouse embryos have demonstrated that ZFR absence leads to developmental defects in certain embryonic structures, an increase in programmed cell death, and a decrease in the mitotic index, underscoring ZFR′s essential role in early development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZFR Antibody (B-8) References:
- Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning. | Hu, RM., et al. 2000. Proc Natl Acad Sci U S A. 97: 9543-8. PMID: 10931946
- Cloning and expression of the human single copy homologue of the mouse zinc finger protein zfr. | Kleines, M., et al. 2001. Gene. 275: 157-62. PMID: 11574164
- Complete sequencing and characterization of 21,243 full-length human cDNAs. | Ota, T., et al. 2004. Nat Genet. 36: 40-5. PMID: 14702039
- The DNA sequence and comparative analysis of human chromosome 5. | Schmutz, J., et al. 2004. Nature. 431: 268-74. PMID: 15372022
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). | Gerhard, DS., et al. 2004. Genome Res. 14: 2121-7. PMID: 15489334
- Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. | Gauci, S., et al. 2009. Anal Chem. 81: 4493-501. PMID: 19413330
- Lysine acetylation targets protein complexes and co-regulates major cellular functions. | Choudhary, C., et al. 2009. Science. 325: 834-40. PMID: 19608861
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZFR Antibody (B-8) | sc-365743 | 200 µg/ml | $322.00 | |||
ZFR Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-529454 | 200 µg Ab; 10 µg BP | $361.00 | |||
ZFR Antibody (B-8): m-IgGκ BP-HRP Bundle | sc-522350 | 200 µg Ab, 40 µg BP | $361.00 | |||
ZFR Antibody (B-8): m-IgG1 BP-HRP Bundle | sc-543525 | 200 µg Ab; 20 µg BP | $361.00 | |||
ZFR Antibody (B-8) X | sc-365743 X | 200 µg/0.1 ml | $322.00 | |||
ZFR Antibody (B-8) AC | sc-365743 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ZFR Antibody (B-8) HRP | sc-365743 HRP | 200 µg/ml | $322.00 | |||
ZFR Antibody (B-8) FITC | sc-365743 FITC | 200 µg/ml | $336.00 | |||
ZFR Antibody (B-8) PE | sc-365743 PE | 200 µg/ml | $349.00 | |||
ZFR Antibody (B-8) Alexa Fluor® 488 | sc-365743 AF488 | 200 µg/ml | $364.00 | |||
ZFR Antibody (B-8) Alexa Fluor® 546 | sc-365743 AF546 | 200 µg/ml | $364.00 | |||
ZFR Antibody (B-8) Alexa Fluor® 594 | sc-365743 AF594 | 200 µg/ml | $364.00 | |||
ZFR Antibody (B-8) Alexa Fluor® 647 | sc-365743 AF647 | 200 µg/ml | $364.00 | |||
ZFR Antibody (B-8) Alexa Fluor® 680 | sc-365743 AF680 | 200 µg/ml | $364.00 | |||
ZFR Antibody (B-8) Alexa Fluor® 790 | sc-365743 AF790 | 200 µg/ml | $364.00 |