ZFAT Antibody (E-9): sc-398058
- ZFAT Antibody (E-9) is a mouse monoclonal IgG1 κ ZFAT antibody provided at 200 µg/ml
- raised against amino acids 16-290 mapping near the N-terminus of ZFAT of human origin
- ZFAT Antibody (E-9) is recommended for detection of ZFAT of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ZFAT Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-398058 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ZFAT Antibody (E-9) for WB applications. These reagents are now offered in bundles with ZFAT Antibody (E-9) (see ordering information below).
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ZFAT Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZFAT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ZFAT antibody (E-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZFAT, also known as Zinc finger protein 406, is a 1243 amino acid protein characterized by nineteen C2H2-type zinc fingers, which play a crucial role in DNA binding and transcriptional regulation. ZFAT primarily resides in the nucleus, where ZFAT modulates gene expression, particularly genes associated with immune response. ZFAT′s nuclear localization enables interactions with various transcription factors and chromatin remodeling complexes, influencing cellular processes such as differentiation and immune regulation. Single-nucleotide polymorphisms (SNPs) within ZFAT have been linked to increased susceptibility to autoimmune thyroid disease, highlighting ZFAT′s potential role in autoimmunity. ZFAT shows strong expression in kidney, testis, ovary, tonsil, placenta, spleen, and peripheral blood leukocytes, existing in three isoforms due to alternative splicing events, which may further diversify ZFAT′s functional roles in different cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZFAT Antibody (E-9) References:
- Zinc finger peptides for the regulation of gene expression. | Klug, A. 1999. J Mol Biol. 293: 215-8. PMID: 10529348
- SNPs in the promoter of a B cell-specific antisense transcript, SAS-ZFAT, determine susceptibility to autoimmune thyroid disease. | Shirasawa, S., et al. 2004. Hum Mol Genet. 13: 2221-31. PMID: 15294872
- Multiple modes of RNA recognition by zinc finger proteins. | Hall, TM. 2005. Curr Opin Struct Biol. 15: 367-73. PMID: 15963892
- [Susceptibility genes for the development of autoimmune thyroid disease]. | Shirasawa, S. 2006. Nihon Rinsho. 64: 2208-14. PMID: 17154080
- ZFAT expression in B and T lymphocytes and identification of ZFAT-regulated genes. | Koyanagi, M., et al. 2008. Genomics. 91: 451-7. PMID: 18329245
- ZFAT is an antiapoptotic molecule and critical for cell survival in MOLT-4 cells. | Fujimoto, T., et al. 2009. FEBS Lett. 583: 568-72. PMID: 19162026
- Genome-wide scan of 500,000 single-nucleotide polymorphisms among responders and nonresponders to interferon beta therapy in multiple sclerosis. | Comabella, M., et al. 2009. Arch Neurol. 66: 972-8. PMID: 19667218
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZFAT Antibody (E-9) | sc-398058 | 200 µg/ml | $322.00 | |||
ZFAT Antibody (E-9): m-IgG Fc BP-HRP Bundle | sc-530684 | 200 µg Ab; 10 µg BP | $361.00 | |||
ZFAT Antibody (E-9): m-IgGκ BP-HRP Bundle | sc-524204 | 200 µg Ab, 40 µg BP | $361.00 | |||
ZFAT Antibody (E-9): m-IgG1 BP-HRP Bundle | sc-544198 | 200 µg Ab; 20 µg BP | $361.00 | |||
ZFAT Antibody (E-9) X | sc-398058 X | 200 µg/0.1 ml | $322.00 | |||
ZFAT Antibody (E-9) AC | sc-398058 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ZFAT Antibody (E-9) HRP | sc-398058 HRP | 200 µg/ml | $322.00 | |||
ZFAT Antibody (E-9) FITC | sc-398058 FITC | 200 µg/ml | $336.00 | |||
ZFAT Antibody (E-9) PE | sc-398058 PE | 200 µg/ml | $349.00 | |||
ZFAT Antibody (E-9) Alexa Fluor® 488 | sc-398058 AF488 | 200 µg/ml | $364.00 | |||
ZFAT Antibody (E-9) Alexa Fluor® 546 | sc-398058 AF546 | 200 µg/ml | $364.00 | |||
ZFAT Antibody (E-9) Alexa Fluor® 594 | sc-398058 AF594 | 200 µg/ml | $364.00 | |||
ZFAT Antibody (E-9) Alexa Fluor® 647 | sc-398058 AF647 | 200 µg/ml | $364.00 | |||
ZFAT Antibody (E-9) Alexa Fluor® 680 | sc-398058 AF680 | 200 µg/ml | $364.00 | |||
ZFAT Antibody (E-9) Alexa Fluor® 790 | sc-398058 AF790 | 200 µg/ml | $364.00 |