ZCSL3 Antibody (E-3): sc-166811
- ZCSL3 Antibody (E-3) is a mouse monoclonal IgG2a κ ZCSL3 antibody provided at 200 µg/ml
- raised against amino acids 1-148 representing full length ZCSL3 of human origin
- ZCSL3 Antibody (E-3) is recommended for detection of ZCSL3 of human origin by WB, IP, IF and ELISA
- Anti-ZCSL3 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-166811 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ZCSL3 Antibody (E-3) for WB applications. These reagents are now offered in bundles with ZCSL3 Antibody (E-3) (see ordering information below).
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ZCSL3 Antibody (E-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects ZCSL3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZCSL3 (E-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZCSL3, a member of the CSL-type zinc finger-containing proteins, plays a crucial role in the synthesis of diphthamide, a post-translational modification found on translation elongation factor EF-2, which is essential for protein synthesis and is a target for bacterial ADP-ribosylating toxins. This modification is vital for proper EF-2 functioning, as diphthamide influences the fidelity and efficiency of protein translation, thereby impacting cellular growth and stress response. ZCSL3, along with ZCSL1 and ZCSL2 homologs, contributes to diphthamide synthesis, with ZCSL2 being particularly important in initial pathway steps. ZCSL2 expression is notably high in spleen, heart, lung, liver, and thymus, indicating significance in diverse biological functions. Multiple format options for anti-ZCSL3 antibody (E-3) enable versatile research applications, supporting studies of diphthamide synthesis and its implications in cellular processes and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZCSL3 Antibody (E-3) References:
- Retroviral insertional mutagenesis identifies a small protein required for synthesis of diphthamide, the target of bacterial ADP-ribosylating toxins. | Liu, S. and Leppla, SH. 2003. Mol Cell. 12: 603-13. PMID: 14527407
- Characterisation of an evolutionary conserved protein interacting with the putative guanine nucleotide exchange factor DelGEF and modulating secretion. | Sjölinder, M., et al. 2004. Exp Cell Res. 294: 68-76. PMID: 14980502
- Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. | Liu, S., et al. 2004. Mol Cell Biol. 24: 9487-97. PMID: 15485916
- A genomewide analysis of genes for the heat shock protein 70 chaperone system in the ascidian Ciona intestinalis. | Wada, S., et al. 2006. Cell Stress Chaperones. 11: 23-33. PMID: 16572726
- Dph3, a small protein required for diphthamide biosynthesis, is essential in mouse development. | Liu, S., et al. 2006. Mol Cell Biol. 26: 3835-41. PMID: 16648478
- The consensus coding sequences of human breast and colorectal cancers. | Sjöblom, T., et al. 2006. Science. 314: 268-74. PMID: 16959974
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZCSL3 Antibody (E-3) | sc-166811 | 200 µg/ml | $316.00 | |||
ZCSL3 Antibody (E-3): m-IgG Fc BP-HRP Bundle | sc-529056 | 200 µg Ab; 10 µg BP | $354.00 | |||
ZCSL3 Antibody (E-3): m-IgGκ BP-HRP Bundle | sc-521755 | 200 µg Ab, 40 µg BP | $354.00 | |||
ZCSL3 Antibody (E-3): m-IgG2a BP-HRP Bundle | sc-547249 | 200 µg Ab; 10 µg BP | $354.00 | |||
ZCSL3 Antibody (E-3) X | sc-166811 X | 200 µg/0.1 ml | $316.00 | |||
ZCSL3 Antibody (E-3) AC | sc-166811 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ZCSL3 Antibody (E-3) HRP | sc-166811 HRP | 200 µg/ml | $316.00 | |||
ZCSL3 Antibody (E-3) FITC | sc-166811 FITC | 200 µg/ml | $330.00 | |||
ZCSL3 Antibody (E-3) PE | sc-166811 PE | 200 µg/ml | $343.00 | |||
ZCSL3 Antibody (E-3) Alexa Fluor® 488 | sc-166811 AF488 | 200 µg/ml | $357.00 | |||
ZCSL3 Antibody (E-3) Alexa Fluor® 546 | sc-166811 AF546 | 200 µg/ml | $357.00 | |||
ZCSL3 Antibody (E-3) Alexa Fluor® 594 | sc-166811 AF594 | 200 µg/ml | $357.00 | |||
ZCSL3 Antibody (E-3) Alexa Fluor® 647 | sc-166811 AF647 | 200 µg/ml | $357.00 | |||
ZCSL3 Antibody (E-3) Alexa Fluor® 680 | sc-166811 AF680 | 200 µg/ml | $357.00 | |||
ZCSL3 Antibody (E-3) Alexa Fluor® 790 | sc-166811 AF790 | 200 µg/ml | $357.00 |