XPA Antibody (B-1): sc-28353

XPA Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects XPA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-XPA antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. XPA protein plays a critical role in the nucleotide excision repair (NER) pathway, which is essential for repairing DNA damage caused by ultraviolet (UV) light. This function is particularly important as deficiencies in NER can lead to xeroderma pigmentosum, a genetic disorder that significantly increases the risk of skin cancer due to the accumulation of unrepaired DNA lesions. XPA is responsible for coordinating the assembly of the preincision complex necessary for recognizing and excising damaged DNA, and XPA exhibits a unique ability to bind to both single-stranded and double-stranded DNA, enhancing efficiency in damage recognition. Furthermore, XPA′s ability to form homodimers in the absence of DNA and transition to a more effective dimeric form upon binding to DNA underscores the regulatory mechanisms that control activity in response to DNA damage. XPA′s importance in maintaining genomic integrity highlights potential as a target for therapeutic interventions in conditions associated with impaired DNA repair mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

XPA Antibody (B-1) References:

1. The relative expression of mutated XPB genes results in xeroderma pigmentosum/Cockayne's syndrome or trichothiodystrophy cellular phenotypes.  |  Riou, L., et al. 1999. Hum Mol Genet. 8: 1125-33. PMID: 10332046
2. Photobiologic and photoimmunologic characteristics of XPA gene-deficient mice.  |  Horio, T., et al. 2001. J Investig Dermatol Symp Proc. 6: 58-63. PMID: 11764287
3. Xeroderma pigmentosum complementation group A protein (XPA) modulates RPA-DNA interactions via enhanced complex stability and inhibition of strand separation activity.  |  Patrick, SM. and Turchi, JJ. 2002. J Biol Chem. 277: 16096-101. PMID: 11859086
4. Cooperative interaction of human XPA stabilizes and enhances specific binding of XPA to DNA damage.  |  Liu, Y., et al. 2005. Biochemistry. 44: 7361-8. PMID: 15882075
5. Opposing effects of the UV lesion repair protein XPA and UV bypass polymerase eta on ATR checkpoint signaling.  |  Bomgarden, RD., et al. 2006. EMBO J. 25: 2605-14. PMID: 16675950
6. ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation.  |  Wu, X., et al. 2007. Oncogene. 26: 757-64. PMID: 16862173
7. XPA deficiency affects the ubiquitin-proteasome system function.  |  de Sousa Leal, AM., et al. 2020. DNA Repair (Amst). 94: 102937. PMID: 32693352
8. Lesion recognition by XPC, TFIIH and XPA in DNA excision repair.  |  Kim, J., et al. 2023. Nature. 617: 170-175. PMID: 37076618
9. Cross-species investigation into the requirement of XPA for nucleotide excision repair.  |  Kose, C., et al. 2024. Nucleic Acids Res. 52: 677-689. PMID: 37994737
10. High incidence of ultraviolet-B-or chemical-carcinogen-induced skin tumours in mice lacking the xeroderma pigmentosum group A gene.  |  Nakane, H., et al. 1995. Nature. 377: 165-8. PMID: 7675085
11. Identification of a damaged-DNA binding domain of the XPA protein.  |  Kuraoka, I., et al. 1996. Mutat Res. 362: 87-95. PMID: 8538652
12. Separation of protein factors that correct the defects in the seven complementation groups of xeroderma pigmentosum cells.  |  Tateishi, S., et al. 1995. J Biochem. 118: 819-24. PMID: 8576098

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-XPA Antibody (B-1) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-XPA Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

XPA Antibody (B-1) Specifications

Alternate Product Name: XPA

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-273

Epitope Target: XPA

Epitope Species: human

Product Reactivity/Detection: XPA

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for XPA Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin