XPA Antibody (2A4): sc-53467
- XPA Antibody (2A4) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against recombinant human XPA protein
- recommended for detection of XPA of human origin by WB
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for XPA Antibody (2A4) for WB applications. These reagents are now offered in bundles with XPA Antibody (2A4) (see ordering information below).
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XPA Antibody (2A4) is a mouse monoclonal IgG1 antibody that detects Xeroderma pigmentosum group A (XPA) in human samples through western blotting (WB). XPA plays a crucial role in the nucleotide excision repair (NER) pathway, which is essential for the cellular response to DNA damage caused by ultraviolet (UV) radiation. XPA protein is vital for maintaining genomic stability, as XPA coordinates the assembly of a preincision complex necessary for the repair of damaged DNA. XPA′s ability to recognize and bind to DNA is influenced by the structural integrity of the DNA double helix and the presence of bulky adducts, making XPA a key player in the repair process. Notably, XPA exists as a homodimer in the absence of DNA, but XPA′s binding efficiency increases significantly when interacting with DNA, highlighting the importance of its dimeric form in effective DNA repair. Deficiencies in XPA lead to an inability to repair UV-induced DNA damage, resulting in a heightened risk of skin cancers in affected individuals. Anti-XPA antibody (2A4) is an invaluable tool for researchers studying the mechanisms of DNA repair and the implications of XPA in xeroderma pigmentosum and related disorders.
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XPA Antibody (2A4) References:
- Critical DNA damage recognition functions of XPC-hHR23B and XPA-RPA in nucleotide excision repair. | Thoma, BS. and Vasquez, KM. 2003. Mol Carcinog. 38: 1-13. PMID: 12949838
- Defective mitophagy in XPA via PARP-1 hyperactivation and NAD(+)/SIRT1 reduction. | Fang, EF., et al. 2014. Cell. 157: 882-896. PMID: 24813611
- Transcriptional consequences of XPA disruption in human cell lines. | Manandhar, M., et al. 2017. DNA Repair (Amst). 57: 76-90. PMID: 28704716
- A key interaction with RPA orients XPA in NER complexes. | Topolska-Woś, AM., et al. 2020. Nucleic Acids Res. 48: 2173-2188. PMID: 31925419
- XPA is susceptible to proteolytic cleavage by cathepsin L during lysis of quiescent cells. | Khan, S., et al. 2022. DNA Repair (Amst). 109: 103260. PMID: 34883264
- XPA Enhances Temozolomide Resistance of Glioblastoma Cells by Promoting Nucleotide Excision Repair. | Dai, W., et al. 2022. Cell Transplant. 31: 9636897221092778. PMID: 35536165
- Two interaction surfaces between XPA and RPA organize the preincision complex in nucleotide excision repair. | Kim, M., et al. 2022. Proc Natl Acad Sci U S A. 119: e2207408119. PMID: 35969784
- Cell migration is impaired in XPA-deficient cells. | Takeuchi, S., et al. 2023. FASEB Bioadv. 5: 53-61. PMID: 36816512
- A disease-associated XPA allele interferes with TFIIH binding and primarily affects transcription-coupled nucleotide excision repair. | van den Heuvel, D., et al. 2023. Proc Natl Acad Sci U S A. 120: e2208860120. PMID: 36893274
- Relationship between XPA, XPB/ERCC3, XPF/ERCC4, and XPG/ERCC5 Polymorphisms and the Susceptibility to Head and Neck Carcinoma: A Systematic Review, Meta-Analysis, and Trial Sequential Analysis. | Imani, MM., et al. 2024. Medicina (Kaunas). 60: PMID: 38541204
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XPA Antibody (2A4) | sc-53467 | 200 µg/ml | $322.00 | |||
XPA Antibody (2A4): m-IgG Fc BP-HRP Bundle | sc-536969 | 200 µg Ab; 10 µg BP | $361.00 | |||
XPA Antibody (2A4): m-IgGκ BP-HRP Bundle | sc-534146 | 200 µg Ab; 40 µg BP | $361.00 | |||
XPA Antibody (2A4): m-IgG1 BP-HRP Bundle | sc-544929 | 200 µg Ab; 20 µg BP | $361.00 |