XIAP Antibody (A-7) recommended for detection of XIAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

XIAP Antibody (A-7): sc-55550

XIAP Antibody (A-7) is rated 5.0 out of 5 by 12.
  • y_2020, m_5, d_28, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.8
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_en_US, sid_55550, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 145ms
  • REVIEWS, PRODUCT
5
12
4
0
3
0
2
0
1
0

Datasheets

    • Anti-XIAP Antibody (A-7) is a mouse monoclonal IgG1 (kappa light chain) XIAP antibody provided at 200 µg/ml
    • raised against amino acids 1-202 mapping at the N-terminus of XIAP of human origin
    • Anti-XIAP Antibody (A-7) is recommended for detection of XIAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-XIAP Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of XIAP (A-7): sc-55550.
Submit a review for this product and receive 15 CruzCredits

What blocking solution should I use with XIAP (A-7): sc-55550 monoclonal antibody for Western blot?

Asked by: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-01-31
  • y_2020, m_5, d_27, h_15CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.8
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_55550, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 141ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Muy buena señal Solicitamos una muestra gratuita de este anticuerpo y la señal obtenida fue muy buena en lisados de células A-549.
Date published: 2018-12-13
Rated 5 out of 5 by from 抗体效果良好 一个月前使用此产品,用于IP实验,0.5ug蛋白对应1ug抗体,效果良好,值得推荐 (值得注意的是,XIAP为55KD,靠近重链位置,可能会有一定干扰)
Date published: 2017-12-13
Rated 5 out of 5 by from experimental data We used 1/200 dilution in rat kidney tissue to do Wb experiments with low background. This result proved our research direction.
Date published: 2017-10-31
Rated 5 out of 5 by from stripe is clear I have use the antibody for westernblotting,and the result is so good,the stripe is clear.
Date published: 2017-10-10
Rated 5 out of 5 by from wonderful antibody! 1:1000 diluted for wb of rat protein ,the result is satisified!
Date published: 2017-05-04
Rated 5 out of 5 by from Really good for western blotting We used this antibody in dilution of 1:1000 to detect XIAP in human cancer cells, and we got good results!
Date published: 2017-04-18
Rated 5 out of 5 by from Good For Western Blot We used this antibody for western blot in Jurkat cells and got a clear distinct band
Date published: 2016-12-21
Rated 5 out of 5 by from Great Antibody-clean bands Antibody: XIAP (A-7), sc-55550 (Mouse monoclonal, Santa Cruz), 1:1000 diluted in 1% Skimmed Milk-TBST. Overnight incubation @ 4°C Samples: SH-SY5Y cell lysates 20µg/lane, 12% Gel Transferred to PVDF membrane; blocked with 5% skimmed milk for 1hr @ rt. Clean bands @ right MW
Date published: 2016-12-04
  • y_2020, m_5, d_28, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.8
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_en_US, sid_55550, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 41ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.