XAP2 Antibody (35-2): sc-59730

XAP2 Antibody (35-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects XAP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). XAP2 Antibody (35-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. XAP2, also known as hepatitis B virus X-associated protein, plays a crucial role in the stabilization and regulation of the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor essential for mediating cellular responses to environmental toxins. XAP2 is primarily located in the cytoplasm, where XAP2 associates with heat shock proteins, including HSP 90, to maintain AhR in an inactive state until ligand binding occurs. Upon activation by dioxins or similar xenobiotics, AhR translocates to the nucleus, forming a heterodimer with Arnt that initiates the transcription of genes involved in xenobiotic metabolism. The interaction between XAP2 and AhR is vital for proper functioning of this signaling pathway, as XAP2 not only stabilizes AhR but may also influence AhR degradation, thereby ensuring a balanced response to environmental stressors.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

XAP2 Antibody (35-2) References:

1. The immunophilin-like protein XAP2 regulates ubiquitination and subcellular localization of the dioxin receptor.  |  Kazlauskas, A., et al. 2000. J Biol Chem. 275: 41317-24. PMID: 11013261
2. Two distinct regions of the immunophilin-like protein XAP2 regulate dioxin receptor function and interaction with hsp90.  |  Kazlauskas, A., et al. 2002. J Biol Chem. 277: 11795-801. PMID: 11805120
3. The role of chaperone proteins in the aryl hydrocarbon receptor core complex.  |  Petrulis, JR. and Perdew, GH. 2002. Chem Biol Interact. 141: 25-40. PMID: 12213383
4. Effect of ARA9 on dioxin receptor mediated transcription.  |  Lees, MJ. and Whitelaw, ML. 2002. Toxicology. 181-182: 143-6. PMID: 12505299
5. Defining the role for XAP2 in stabilization of the dioxin receptor.  |  Lees, MJ., et al. 2003. J Biol Chem. 278: 35878-88. PMID: 12837759
6. Hepatitis B virus X-associated protein 2 is a subunit of the unliganded aryl hydrocarbon receptor core complex and exhibits transcriptional enhancer activity.  |  Meyer, BK., et al. 1998. Mol Cell Biol. 18: 978-88. PMID: 9447995
7. Characterization of the Ah receptor-associated protein, ARA9.  |  Carver, LA., et al. 1998. J Biol Chem. 273: 33580-7. PMID: 9837941

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-XAP2 Antibody (35-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-XAP2 Antibody (35-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

XAP2 Antibody (35-2) Specifications

Alternate Product Name: XAP2

Product Clone Name: 35-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: XAP2

Epitope Species: human

Product Reactivity/Detection: XAP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for XAP2 Antibody (35-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin