WRN Antibody (D-6): sc-376182

WRN Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects WRN protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WRN (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WRN protein plays a crucial role in maintaining genomic stability, as WRN is involved in DNA repair processes, particularly in unwinding DNA during replication and repair. This function is vital because defects in WRN protein can lead to DNA damage accumulation, which is a hallmark of aging and is associated with age-related diseases, including cancer. Werner′s syndrome, an autosomal recessive disorder linked to mutations in WRN gene, exemplifies WRN protein′s importance; individuals with this syndrome experience premature aging and a higher incidence of malignancies. WRN gene, located on chromosome 8′s short arm, encodes a protein that is 1,432 amino acids long and shares significant homology with other DNA helicases. Notably, four mutations in WRN gene have been identified in patients with Werner′s syndrome, with one mutation affecting mRNA splice-junctions found in 60% of those examined, leading to a frameshift and a truncated WRN protein.

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WRN Antibody (D-6) References:

1. WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome.  |  Tian, Y., et al. 2022. Nat Commun. 13: 5456. PMID: 36114168
2. Clinical prospects of WRN inhibition as a treatment for MSI tumours.  |  Morales-Juarez, DA. and Jackson, SP. 2022. NPJ Precis Oncol. 6: 85. PMID: 36379964
3. WRN helicase and mismatch repair complexes independently and synergistically disrupt cruciform DNA structures.  |  Mengoli, V., et al. 2023. EMBO J. 42: e111998. PMID: 36541070
4. Discovery of thiophen-2-ylmethylene bis-dimedone derivatives as novel WRN inhibitors for treating cancers with microsatellite instability.  |  Yang, H., et al. 2024. Bioorg Med Chem. 100: 117588. PMID: 38295487
5. WRN exonuclease imparts high fidelity on translesion synthesis by Y family DNA polymerases.  |  Yoon, JH., et al. 2024. Genes Dev. 38: 213-232. PMID: 38503516
6. Genetic association between chromosome 8 microsatellite (MS8-134) and Werner syndrome (WRN): chromosome microdissection and homozygosity mapping.  |  Ye, L., et al. 1995. Genomics. 28: 566-9. PMID: 7490095
7. Homozygosity mapping of the Werner syndrome locus (WRN).  |  Nakura, J., et al. 1994. Genomics. 23: 600-8. PMID: 7851888
8. Linkage disequilibrium and haplotype studies of chromosome 8p 11.1-21.1 markers and Werner syndrome.  |  Yu, CE., et al. 1994. Am J Hum Genet. 55: 356-64. PMID: 8037212
9. A genetic analysis of the Werner syndrome region on human chromosome 8p.  |  Thomas, W., et al. 1993. Genomics. 16: 685-90. PMID: 8325642
10. Positional cloning of the Werner's syndrome gene.  |  Yu, CE., et al. 1996. Science. 272: 258-62. PMID: 8602509
11. Toward localization of the Werner syndrome gene by linkage disequilibrium and ancestral haplotyping: lessons learned from analysis of 35 chromosome 8p11.1-21.1 markers.  |  Goddard, KA., et al. 1996. Am J Hum Genet. 58: 1286-302. PMID: 8651307
12. Excess of rare cancers in Werner syndrome (adult progeria).  |  Goto, M., et al. 1996. Cancer Epidemiol Biomarkers Prev. 5: 239-46. PMID: 8722214

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WRN Antibody (D-6) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-WRN Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WRN Antibody (D-6) Specifications

Alternate Product Name: Werner Syndrome

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1133-1432

Epitope Target: WRN

Epitope Species: human

Product Reactivity/Detection: WRN

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for WRN Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin