Anti-WRN Antibody (D-6) is a mouse monoclonal IgG1 (kappa light chain) WRN antibody provided at 200 µg/ml
raised against amino acids 1133-1432 of WRN of human origin
WRN Antibody (D-6) is recommended for detection of WRN of human origin by WB, IP, IF and ELISA
Anti-WRN Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of WRN (D-6): sc-376182.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
WRN Antibody (D-6) is a high quality monoclonal WRN antibody (also designated WRN antibody) suitable for the detection of the WRN protein of human origin. WRN Antibody (D-6) is available as both the non-conjugated anti-WRN antibody form, as well as multiple conjugated forms of anti-WRN antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Werner′s syndrome (WS), also called adult progeria, is an inherited, autosomal recessive disorder that is most common in families from regions of Japan where consanguineous marriages occur frequently. WS is characterized by premature aging and the early onset of age-related diseases and commonly results in cancer. The gene responsible for Werner′s syndrome, WRN, has been mapped to the short arm of chromosome 8, and the subsequent cloning of the gene has reveled a predicted protein of 1,432 amino acids in length that bears significant sequence homology with DNA helicases. Four mutations in WRN have been identified in patients afflicted with WS. Two of the mutations involve mRNA splice-junctions. Of these two mutations, one was found in 60% of the individuals examined. This mutation is predicted to cause a frameshift which results in a truncated WRN protein.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for WRN (D-6): sc-376182 monoclonal antibody?
Asked by: cjMara
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link:
If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
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