Wnt-3 Antibody (D-9): sc-74537

Wnt-3 Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Wnt-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Wnt-3 (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Wnt-3 protein plays a crucial role in developmental processes, particularly in regulating cell fate and patterning during embryogenesis, by acting as a signaling molecule that binds to frizzled receptors. This interaction is vital for stabilizing cytoplasmic beta-catenin, which is essential for the Wnt-1/wingless signaling pathway, influencing oncogenesis and various cellular functions. Wnt-3 is specifically expressed in the Purkinje cell layer of the adult cerebellum and serves as a retrograde signal in motoneurons, controlling terminal branching of muscle afferents, which is critical for proper motor function. Human Wnt-3 exhibits 98% homology with mouse Wnt-3 and 84% with human Wnt-3a, indicating a high degree of evolutionary conservation, and human Wnt-3 gene is located in close proximity to Wnt-15 gene on chromosome 17q21, highlighting its significance in the Wnt signaling pathway.

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Wnt-3 Antibody (D-9) References:

1. The Wnt/Ca2+ pathway: a new vertebrate Wnt signaling pathway takes shape.  |  Kühl, M., et al. 2000. Trends Genet. 16: 279-83. PMID: 10858654
2. WNT-3, expressed by motoneurons, regulates terminal arborization of neurotrophin-3-responsive spinal sensory neurons.  |  Krylova, O., et al. 2002. Neuron. 35: 1043-56. PMID: 12354395
3. Regional expression of the Wnt-3 gene in the developing mouse forebrain in relationship to diencephalic neuromeres.  |  Salinas, PC. and Nusse, R. 1992. Mech Dev. 39: 151-60. PMID: 1363370
4. Proliferation of neural stem cells correlates with Wnt-3 protein in hypoxic-ischemic neonate rats after hyperbaric oxygen therapy.  |  Wang, XL., et al. 2007. Neuroreport. 18: 1753-6. PMID: 17921881
5. Wnt-3a and Wnt-3 differently stimulate proliferation and neurogenesis of spinal neural precursors and promote neurite outgrowth by canonical signaling.  |  David, MD., et al. 2010. J Neurosci Res. 88: 3011-23. PMID: 20722074
6. Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and adult brain.  |  Roelink, H., et al. 1990. Proc Natl Acad Sci U S A. 87: 4519-23. PMID: 2162045
7. Identification of Noncanonical Wnt Receptors Required for Wnt-3a-Induced Early Differentiation of Human Neural Stem Cells.  |  Bengoa-Vergniory, N., et al. 2017. Mol Neurobiol. 54: 6213-6224. PMID: 27709494
8. Neuroprotective and regenerative roles of intranasal Wnt-3a administration after focal ischemic stroke in mice.  |  Wei, ZZ., et al. 2018. J Cereb Blood Flow Metab. 38: 404-421. PMID: 28430000
9. Wnt-3a alleviates neuroinflammation after ischemic stroke by modulating the responses of microglia/macrophages and astrocytes.  |  Zhang, D., et al. 2019. Int Immunopharmacol. 75: 105760. PMID: 31323530
10. Wnt-3a Induces Epigenetic Remodeling in Human Dental Pulp Stem Cells.  |  Uribe-Etxebarria, V., et al. 2020. Cells. 9: PMID: 32156036
11. Wnt3a Facilitates SARS-CoV-2 Pseudovirus Entry into Cells.  |  Melano, I., et al. 2023. Int J Mol Sci. 25: PMID: 38203386
12. Maintenance of Wnt-3 expression in Purkinje cells of the mouse cerebellum depends on interactions with granule cells.  |  Salinas, PC., et al. 1994. Development. 120: 1277-86. PMID: 8026336

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Wnt-3 Antibody (D-9) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-Wnt-3 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Wnt-3 Antibody (D-9) Specifications

Alternate Product Name: WNT3

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-310

Epitope Target: Wnt-3a

Epitope Species: human

Product Reactivity/Detection: precursor and mature Wnt-3 and Wnt-3a

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for Wnt-3 Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Wnt-3a antibody is Wnt-3a Antibody (3A6): sc-136163.