Wnt-2 Antibody (E-7): sc-514382

Wnt-2 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Wnt-2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Wnt-2 antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Wnt-2, a member of the highly conserved Wnt gene family, plays a crucial role in cellular signaling pathways that regulate cell growth, differentiation, and tissue homeostasis. Specifically, Wnt-2 is involved in the development of the embryonic nervous system and has been implicated in various cancers, including breast cancer, where aberrant expression can lead to uncontrolled cell proliferation. Wnt-2 is secreted and interacts with Frizzled receptors on the cell surface, initiating a cascade of intracellular signaling that influences gene expression and cellular behavior. Understanding Wnt-2 function is vital, as Wnt-2 not only contributes to normal developmental processes but also has significant implications in tumorigenesis, making Wnt-2 a critical target for therapeutic interventions in cancer treatment.

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Wnt-2 Antibody (E-7) References:

1. Inhibition of Wnt-2-mediated signaling induces programmed cell death in non-small-cell lung cancer cells.  |  You, L., et al. 2004. Oncogene. 23: 6170-4. PMID: 15208662
2. Wnt genes.  |  Nusse, R. and Varmus, HE. 1992. Cell. 69: 1073-87. PMID: 1617723
3. Frizzled-8 receptor is activated by the Wnt-2 ligand in non-small cell lung cancer.  |  Bravo, DT., et al. 2013. BMC Cancer. 13: 316. PMID: 23815780
4. Cadherin 11 Inhibition Downregulates β-catenin, Deactivates the Canonical WNT Signalling Pathway and Suppresses the Cancer Stem Cell-Like Phenotype of Triple Negative Breast Cancer.  |  Satriyo, PB., et al. 2019. J Clin Med. 8: PMID: 30691241
5. Capsaicin suppressed activity of prostate cancer stem cells by inhibition of Wnt/β-catenin pathway.  |  Zhu, M., et al. 2020. Phytother Res. 34: 817-824. PMID: 31782192
6. Combinatorial signaling by Sonic hedgehog and Wnt family members induces myogenic bHLH gene expression in the somite.  |  Münsterberg, AE., et al. 1995. Genes Dev. 9: 2911-22. PMID: 7498788
7. Biochemical analysis of murine Wnt proteins reveals both shared and distinct properties.  |  Burrus, LW. and McMahon, AP. 1995. Exp Cell Res. 220: 363-73. PMID: 7556445
8. Differential transformation of mammary epithelial cells by Wnt genes.  |  Wong, GT., et al. 1994. Mol Cell Biol. 14: 6278-86. PMID: 8065359
9. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue.  |  Huguet, EL., et al. 1994. Cancer Res. 54: 2615-21. PMID: 8168088
10. Properties of Wnt-1 protein that enable cell surface association.  |  Schryver, B., et al. 1996. Oncogene. 13: 333-42. PMID: 8710372
11. MMTV-induced mutations in mouse mammary tumors: their potential relevance to human breast cancer.  |  Callahan, R. 1996. Breast Cancer Res Treat. 39: 33-44. PMID: 8738604
12. Characterization of Wnt-1 and Wnt-2 induced growth alterations and signaling pathways in NIH3T3 fibroblasts.  |  Bafico, A., et al. 1998. Oncogene. 16: 2819-25. PMID: 9652750

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Wnt-2 Antibody (E-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Wnt-2 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Wnt-2 Antibody (E-7) Specifications

Alternate Product Name: WNT2

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 231-270

Epitope Target: Wnt-2

Epitope Species: human

Product Reactivity/Detection: precursor and mature Wnt-2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Wnt-2 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin