Wnt-10a Antibody (A-4) recommended for detection of Wnt-10a of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Wnt-10a Antibody (A-4): sc-376028

Wnt-10a Antibody (A-4) is rated 3.8 out of 5 by 6.
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Datasheets

    • Wnt-10a Antibody (A-4) is a mouse monoclonal IgG2a (kappa light chain) Wnt-10a antibody provided at 200 µg/ml
    • raised against amino acids 153-229 mapping within an internal region of Wnt-10a of human origin
    • Wnt-10a Antibody (A-4) is recommended for detection of Wnt-10a of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Wnt-10a Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Wnt-10a (A-4): sc-376028.
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Can Wnt-10a (A-4): sc-376028 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, Wnt-10a (A-4): sc-376028 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 1 out of 5 by from Did not work for WB I tried using this antibody at 1:100 O/N and saw a partial band but was not very clear.
Date published: 2018-05-18
Rated 4 out of 5 by from Funcks well! About one month ago, we bought this antibody, we found it worked well inspite of the not-very-clear background.
Date published: 2017-06-25
Rated 4 out of 5 by from 挺好的 贵公司的服务周到,为客户解决问题,包装严实,顺风快递给力,这个抗体暂时还没使用,应该效果不错,给好评
Date published: 2017-06-02
Rated 5 out of 5 by from We used this in Western Blot and IHC and We used this in Western Blot and IHC and we were very pleased.
Date published: 2015-07-29
Rated 4 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-05-14
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmic Produced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human smooth muscle tissue. -SCBT QC
Date published: 2015-03-24
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