WIPI-4 Antibody (G-12): sc-398272

WIPI-4 Antibody (G-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects WIPI-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WIPI-4 (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WIPI-4 protein, also known as WD repeat domain phosphoinositide-interacting protein 4 or WDR45, is a 360 amino acid protein characterized by the presence of three WD repeats, which are structural motifs that facilitate protein-protein interactions. These WD repeats contribute to WIPI-4′s ability to engage in critical cellular processes, including autophagy and endosomal trafficking, by mediating interactions with phosphoinositides. WIPI-4′s importance lies in regulating autophagy, a vital cellular mechanism for degrading and recycling cellular components, which is essential for maintaining cellular homeostasis and responding to stress. WIPI-4 is ubiquitously expressed, with highest levels found in heart and skeletal muscle, and exists in three alternatively spliced isoforms, highlighting diverse functional roles across different tissues.

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WIPI-4 Antibody (G-12) References:

1. Inferring nonneutral evolution from human-chimp-mouse orthologous gene trios.  |  Clark, AG., et al. 2003. Science. 302: 1960-3. PMID: 14671302
2. PtdIns-specific MPR pathway association of a novel WD40 repeat protein, WIPI49.  |  Jeffries, TR., et al. 2004. Mol Biol Cell. 15: 2652-63. PMID: 15020712
3. WIPI-1alpha (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy.  |  Proikas-Cezanne, T., et al. 2004. Oncogene. 23: 9314-25. PMID: 15602573
4. Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy.  |  Proikas-Cezanne, T., et al. 2007. FEBS Lett. 581: 3396-404. PMID: 17618624
5. Phylogenetic, structural and functional relationships between WD- and Kelch-repeat proteins.  |  Hudson, AM. and Cooley, L. 2008. Subcell Biochem. 48: 6-19. PMID: 18925367
6. WDR45 contributes to neurodegeneration through regulation of ER homeostasis and neuronal death.  |  Wan, H., et al. 2020. Autophagy. 16: 531-547. PMID: 31204559
7. WDR45, one gene associated with multiple neurodevelopmental disorders.  |  Cong, Y., et al. 2021. Autophagy. 17: 3908-3923. PMID: 33843443
8. WDR45 Mutation Impairs the Autophagic Degradation of Transferrin Receptor and Promotes Ferroptosis.  |  Xiong, Q., et al. 2021. Front Mol Biosci. 8: 645831. PMID: 34012978
9. A neurodegeneration gene, WDR45, links impaired ferritinophagy to iron accumulation.  |  Aring, L., et al. 2022. J Neurochem. 160: 356-375. PMID: 34837396
10. Mutant WDR45 Leads to Altered Ferritinophagy and Ferroptosis in β-Propeller Protein-Associated Neurodegeneration.  |  Diaw, SH., et al. 2022. Int J Mol Sci. 23: PMID: 36076926
11. WDR45 variants cause ferrous iron loss due to impaired ferritinophagy associated with nuclear receptor coactivator 4 and WD repeat domain phosphoinositide interacting protein 4 reduction.  |  Tsukida, K., et al. 2022. Brain Commun. 4: fcac304. PMID: 36751498
12. WDR45 mutation dysregulates iron homeostasis by promoting the chaperone-mediated autophagic degradation of ferritin heavy chain in an ER stress/p38 dependent mechanism.  |  Xiong, Q., et al. 2023. Free Radic Biol Med. 201: 89-97. PMID: 36940732

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WIPI-4 Antibody (G-12) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-WIPI-4 Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WIPI-4 Antibody (G-12) Specifications

Alternate Product Name: WIPI-4

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 124-149

Epitope Target: WIPI-4

Epitope Species: human

Product Reactivity/Detection: WIPI-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for WIPI-4 Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin