WDR53 Antibody (C-8): sc-514527
- WDR53 Antibody (C-8) is a mouse monoclonal IgG1 κ WDR53 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 69-86 within an internal region of WDR53 of human origin
- WDR53 Antibody (C-8) is recommended for detection of WDR53 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-WDR53 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for WDR53 Antibody (C-8) for WB applications. These reagents are now offered in bundles with WDR53 Antibody (C-8) (see ordering information below).
WDR53 Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects WDR53 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-WDR53 antibody (C-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WDR53 consists of 358 amino acids and features five WD repeats, playing a crucial role in cellular processes by participating in protein complex assembly. WD repeats, characterized by a conserved core of 40-60 amino acids that typically form a tertiary propeller structure, allow WDR53 to engage in protein-protein interactions essential for regulatory mechanisms such as signal transduction, apoptosis, transcriptional regulation, and cell cycle control. WDR53 is encoded on human chromosome 3, a region spanning approximately 214 million bases and containing over 1,100 genes, highlighting potential involvement in various biological pathways and disease states, including cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
WDR53 Antibody (C-8) References:
- The WD repeat: a common architecture for diverse functions. | Smith, TF., et al. 1999. Trends Biochem Sci. 24: 181-5. PMID: 10322433
- Thirty-plus functional families from a single motif. | Yu, L., et al. 2000. Protein Sci. 9: 2470-6. PMID: 11206068
- WD-repeat proteins: structure characteristics, biological function, and their involvement in human diseases. | Li, D. and Roberts, R. 2001. Cell Mol Life Sci. 58: 2085-97. PMID: 11814058
- The WD-repeat protein superfamily in Arabidopsis: conservation and divergence in structure and function. | van Nocker, S. and Ludwig, P. 2003. BMC Genomics. 4: 50. PMID: 14672542
- The consensus coding sequences of human breast and colorectal cancers. | Sjöblom, T., et al. 2006. Science. 314: 268-74. PMID: 16959974
- A quantitative atlas of mitotic phosphorylation. | Dephoure, N., et al. 2008. Proc Natl Acad Sci U S A. 105: 10762-7. PMID: 18669648
- Phylogenetic, structural and functional relationships between WD- and Kelch-repeat proteins. | Hudson, AM. and Cooley, L. 2008. Subcell Biochem. 48: 6-19. PMID: 18925367
- The ancient regulatory-protein family of WD-repeat proteins. | Neer, EJ., et al. 1994. Nature. 371: 297-300. PMID: 8090199
- Folding of proteins with WD-repeats: comparison of six members of the WD-repeat superfamily to the G protein beta subunit. | Garcia-Higuera, I., et al. 1996. Biochemistry. 35: 13985-94. PMID: 8909296
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR53 Antibody (C-8) | sc-514527 | 200 µg/ml | $322.00 | |||
WDR53 Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-531152 | 200 µg Ab; 10 µg BP | $361.00 | |||
WDR53 Antibody (C-8): m-IgGκ BP-HRP Bundle | sc-524757 | 200 µg Ab, 40 µg BP | $361.00 | |||
WDR53 Antibody (C-8): m-IgG1 BP-HRP Bundle | sc-544418 | 200 µg Ab; 20 µg BP | $361.00 | |||
WDR53 Antibody (C-8) AC | sc-514527 AC | 500 µg/ml, 25% agarose | $424.00 | |||
WDR53 Antibody (C-8) HRP | sc-514527 HRP | 200 µg/ml | $322.00 | |||
WDR53 Antibody (C-8) FITC | sc-514527 FITC | 200 µg/ml | $336.00 | |||
WDR53 Antibody (C-8) PE | sc-514527 PE | 200 µg/ml | $349.00 | |||
WDR53 Antibody (C-8) Alexa Fluor® 488 | sc-514527 AF488 | 200 µg/ml | $364.00 | |||
WDR53 Antibody (C-8) Alexa Fluor® 546 | sc-514527 AF546 | 200 µg/ml | $364.00 | |||
WDR53 Antibody (C-8) Alexa Fluor® 594 | sc-514527 AF594 | 200 µg/ml | $364.00 | |||
WDR53 Antibody (C-8) Alexa Fluor® 647 | sc-514527 AF647 | 200 µg/ml | $364.00 | |||
WDR53 Antibody (C-8) Alexa Fluor® 680 | sc-514527 AF680 | 200 µg/ml | $364.00 | |||
WDR53 Antibody (C-8) Alexa Fluor® 790 | sc-514527 AF790 | 200 µg/ml | $364.00 | |||
WDR53 (C-8) Neutralizing Peptide | sc-514527 P | 100 µg/0.5 ml | $69.00 |