WDR5 Antibody (G-9): sc-393080

WDR5 Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects WDR5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-WDR5 antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WD-repeat protein 5 (WDR5), also known as BMP-2-induced gene 3 kb or BIG-3, is a crucial component of the Trithorax (TRX) histone methyltransferase complexes, which are vital for regulating gene expression during vertebrate development. WDR5 plays a significant role in the activation of Hox genes and is essential for global trimethylation of histone H3 at lysine 4 (H3K4me3), a modification associated with active transcription. WDR5 is predominantly located in the nucleus, where WDR5 interacts with histones and other regulatory proteins, facilitating the recruitment of additional factors necessary for chromatin remodeling and gene activation. WDR5 expression is particularly noted in osteoblasts, chondrocytes, osteocytes, and marrow stromal cells, highlighting WDR5′s importance in bone development and differentiation. WDR5′s structure, characterized by seven WD-repeats, is critical for function, as these repeats mediate protein-protein interactions, thereby influencing various cellular processes, including osteoblast differentiation.

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WDR5 Antibody (G-9) References:

1. WDR5 associates with histone H3 methylated at K4 and is essential for H3 K4 methylation and vertebrate development.  |  Wysocka, J., et al. 2005. Cell. 121: 859-72. PMID: 15960974
2. Wdr5, a novel WD repeat protein, regulates osteoblast and chondrocyte differentiation in vivo.  |  Gori, F., et al. 2005. J Musculoskelet Neuronal Interact. 5: 338-9. PMID: 16340128
3. Structural basis for the specific recognition of methylated histone H3 lysine 4 by the WD-40 protein WDR5.  |  Han, Z., et al. 2006. Mol Cell. 22: 137-44. PMID: 16600877
4. A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling.  |  Wysocka, J., et al. 2006. Nature. 442: 86-90. PMID: 16728976
5. Wdr5, a WD-40 protein, regulates osteoblast differentiation during embryonic bone development.  |  Gori, F., et al. 2006. Dev Biol. 295: 498-506. PMID: 16730692
6. Histone H3 recognition and presentation by the WDR5 module of the MLL1 complex.  |  Ruthenburg, AJ., et al. 2006. Nat Struct Mol Biol. 13: 704-12. PMID: 16829959
7. Molecular recognition of histone H3 by the WD40 protein WDR5.  |  Couture, JF., et al. 2006. Nat Struct Mol Biol. 13: 698-703. PMID: 16829960
8. Up-regulated WDR5 promotes gastric cancer formation by induced cyclin D1 expression.  |  Sun, W., et al. 2018. J Cell Biochem. 119: 3304-3316. PMID: 29125890
9. Human WDR5 promotes breast cancer growth and metastasis via KMT2-independent translation regulation.  |  Cai, WL., et al. 2022. Elife. 11: PMID: 36043466
10. Loss of Wdr5 attenuates MLL-rearranged leukemogenesis by suppressing Myc targets.  |  Liu, L., et al. 2023. Biochim Biophys Acta Mol Basis Dis. 1869: 166600. PMID: 36402263
11. Unannotated microprotein EMBOW regulates the interactome and chromatin and mitotic functions of WDR5.  |  Chen, Y., et al. 2023. Cell Rep. 42: 113145. PMID: 37725512
12. The PML1-WDR5 axis regulates H3K4me3 marks and promotes stemness of estrogen receptor-positive breast cancer.  |  Pai, CP., et al. 2024. Cell Death Differ. 31: 768-778. PMID: 38627584

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WDR5 Antibody (G-9) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-WDR5 Antibody (G-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WDR5 Antibody (G-9) Specifications

Alternate Product Name: WDR5

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-35

Epitope Target: WDR5

Epitope Species: human

Product Reactivity/Detection: WDR5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for WDR5 Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin