WDFY3 Antibody (B-4): sc-514569

WDFY3 Antibody (B-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects WDFY3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WDFY3 (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WDFY3 protein, also known as ALFY (autophagy-linked FYVE protein), plays a crucial role in autophagy, which is essential for cellular homeostasis and degradation of damaged organelles and proteins. By facilitating autophagosome formation, WDFY3 helps maintain cellular health, particularly under stress conditions such as nutrient deprivation. WDFY3 protein is composed of 3,526 amino acids and localizes to the cytoplasmic side of peripheral membranes, where WDFY3 colocalizes with autophagic structures in starved cells. WDFY3 is widely expressed in various tissues, including liver, brain, and kidney, and exists in two alternatively spliced isoforms. WDFY3 structural features include one BEACH domain, one FYVE-type zinc finger, a pair of leucine-rich repeats, and five WD repeats, which participate in diverse cellular functions, including chromatin assembly, cell cycle control, and signal transduction. WDFY3′s ability to interact with other proteins involved in these pathways underscores WDFY3′s importance in regulating cellular processes and responding to stress, making anti-WDFY3 antibody (B-4) a valuable tool for researchers studying autophagy and related mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

WDFY3 Antibody (B-4) References:

1. Cellular functions of phosphatidylinositol 3-phosphate and FYVE domain proteins.  |  Gillooly, DJ., et al. 2001. Biochem J. 355: 249-58. PMID: 11284710
2. Structural basis for endosomal targeting by FYVE domains.  |  Hayakawa, A., et al. 2004. J Biol Chem. 279: 5958-66. PMID: 14594806
3. Alfy, a novel FYVE-domain-containing protein associated with protein granules and autophagic membranes.  |  Simonsen, A., et al. 2004. J Cell Sci. 117: 4239-51. PMID: 15292400
4. Expression profile of mouse BWF1, a protein with a BEACH domain, WD40 domain and FYVE domain.  |  Chen, GY., et al. 2004. Cell Struct Funct. 29: 35-42. PMID: 15342963
5. The WD40 and FYVE domain containing protein 2 defines a class of early endosomes necessary for endocytosis.  |  Hayakawa, A., et al. 2006. Proc Natl Acad Sci U S A. 103: 11928-33. PMID: 16873553
6. Tyrosine phosphorylated Par3 regulates epithelial tight junction assembly promoted by EGFR signaling.  |  Wang, Y., et al. 2006. EMBO J. 25: 5058-70. PMID: 17053785
7. Evolutionarily conserved structural and functional roles of the FYVE domain.  |  Hayakawa, A., et al. 2007. Biochem Soc Symp. 95-105. PMID: 17233583
8. FYVE fingers bind PtdIns(3)P.  |  Gaullier, JM., et al. 1998. Nature. 394: 432-3. PMID: 9697764

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WDFY3 Antibody (B-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-WDFY3 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WDFY3 Antibody (B-4) Specifications

Alternate Product Name: WDFY3

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: WDFY3

Epitope Species: human

Product Reactivity/Detection: WDFY3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for WDFY3 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent