VRK2 Antibody (H-5): sc-365199
- VRK2 Antibody (H-5) is a mouse monoclonal IgG1 κ VRK2 antibody, cited in 7 publications, provided at 200 µg/ml
- raised against amino acids 254-496 mapping at the C-terminus of VRK2 of human origin
- VRK2 Antibody (H-5) is recommended for detection of VRK2 of human origin by WB, IP, IF and ELISA
- Anti-VRK2 Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VRK2 Antibody (H-5) for WB applications. These reagents are now offered in bundles with VRK2 Antibody (H-5) (see ordering information below).
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VRK2 Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects VRK2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-VRK2 antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VRK2, or vaccinia related kinase 2, is a 508 amino acid single-pass type IV membrane protein that features a protein kinase domain and is classified within the serine/threonine protein kinase family. VRK2 is predominantly expressed in the heart, skeletal muscle, pancreas, testis, and fetal liver, where VRK2 plays a crucial role as a serine/threonine kinase that catalyzes the ATP-dependent phosphorylation of target proteins, including casein and p53, thereby modulating their functional activities within the cell. VRK2 is localized to the endoplasmic reticulum, which allows VRK2 to effectively regulate protein activity and participate in essential cellular processes such as proliferation. VRK2 expression is found to be upregulated in certain carcinomas, indicating potential involvement in cancer development. Five isoforms of VRK2 arise from alternative splicing events, which may contribute to diverse functional roles in cellular signaling and regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VRK2 Antibody (H-5) References:
- Expression of the VRK (vaccinia-related kinase) gene family of p53 regulators in murine hematopoietic development. | Vega, FM., et al. 2003. FEBS Lett. 544: 176-80. PMID: 12782311
- Characterization of three paralogous members of the Mammalian vaccinia related kinase family. | Nichols, RJ. and Traktman, P. 2004. J Biol Chem. 279: 7934-46. PMID: 14645249
- The vaccinia-related kinases phosphorylate the N' terminus of BAF, regulating its interaction with DNA and its retention in the nucleus. | Nichols, RJ., et al. 2006. Mol Biol Cell. 17: 2451-64. PMID: 16495336
- The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines. | Blanco, S., et al. 2006. FEBS J. 273: 2487-504. PMID: 16704422
- Human cellular protein VRK2 interacts specifically with Epstein-Barr virus BHRF1, a homologue of Bcl-2, and enhances cell survival. | Li, LY., et al. 2006. J Gen Virol. 87: 2869-2878. PMID: 16963744
- Vaccinia-related kinase 2 modulates the stress response to hypoxia mediated by TAK1. | Blanco, S., et al. 2007. Mol Cell Biol. 27: 7273-83. PMID: 17709393
- Modulation of interleukin-1 transcriptional response by the interaction between VRK2 and the JIP1 scaffold protein. | Blanco, S., et al. 2008. PLoS One. 3: e1660. PMID: 18286207
- Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase. | Nezu, J., et al. 1997. Genomics. 45: 327-31. PMID: 9344656
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VRK2 Antibody (H-5) | sc-365199 | 200 µg/ml | $322.00 | |||
VRK2 Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-529325 | 200 µg Ab; 10 µg BP | $361.00 | |||
VRK2 Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-522161 | 200 µg Ab, 40 µg BP | $361.00 | |||
VRK2 Antibody (H-5): m-IgG1 BP-HRP Bundle | sc-543458 | 200 µg Ab; 20 µg BP | $361.00 | |||
VRK2 Antibody (H-5) AC | sc-365199 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VRK2 Antibody (H-5) HRP | sc-365199 HRP | 200 µg/ml | $322.00 | |||
VRK2 Antibody (H-5) FITC | sc-365199 FITC | 200 µg/ml | $336.00 | |||
VRK2 Antibody (H-5) PE | sc-365199 PE | 200 µg/ml | $349.00 | |||
VRK2 Antibody (H-5) Alexa Fluor® 488 | sc-365199 AF488 | 200 µg/ml | $364.00 | |||
VRK2 Antibody (H-5) Alexa Fluor® 546 | sc-365199 AF546 | 200 µg/ml | $364.00 | |||
VRK2 Antibody (H-5) Alexa Fluor® 594 | sc-365199 AF594 | 200 µg/ml | $364.00 | |||
VRK2 Antibody (H-5) Alexa Fluor® 647 | sc-365199 AF647 | 200 µg/ml | $364.00 | |||
VRK2 Antibody (H-5) Alexa Fluor® 680 | sc-365199 AF680 | 200 µg/ml | $364.00 | |||
VRK2 Antibody (H-5) Alexa Fluor® 790 | sc-365199 AF790 | 200 µg/ml | $364.00 |