VRK1 Antibody (E-3): sc-390809

VRK1 Antibody (E-3) is a mouse monoclonal IgG1 antibody that detects VRK1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VRK1, or vaccina-related kinase 1, plays a crucial role in cellular stress responses by phosphorylating key transcription factors, including p53 and c-Jun, which are vital for regulating cell cycle and apoptosis. VRK1 phosphorylation of p53 at Thr18 is particularly significant, as this disrupts the interaction between p53 and its negative regulator, Mdm2, thereby stabilizing p53 and enhancing its tumor suppressor functions. VRK1′s interaction with c-Jun, where phosphorylation occurs at specific serine residues, activates c-Jun-dependent transcription, further influencing cellular responses to stress. Nuclear localization of VRK1 is essential for direct interaction with these transcription factors, underscoring VRK1′s importance in maintaining cellular homeostasis and responding to stress signals. Through modulation of critical pathways, VRK1 serves as a key player in cancer biology and cellular response mechanisms, making anti-VRK1 antibody (E-3) an invaluable tool for researchers studying these processes.

The preferred VRK1 antibody is VRK1 Antibody (A-11): sc-271061.