VR1 Antibody (E-8): sc-398417

VR1 Antibody (E-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects VR1 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VR1 (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The vanilloid receptor 1 (VR1), also known as the capsaicin receptor, is a nonselective cation channel that plays a crucial role in pain perception and is structurally related to the transient receptor potential (TRP) family of ion channels. VR1 is primarily located in primary sensory neurons and vagal nerves, where VR1 serves as a key molecular integrator of various chemical and physical stimuli that can induce pain. Notably, VR1 is activated by capsaicin, the active component of chili peppers, as well as by heat and acidic conditions that arise during inflammation or ischemia. The ability of protons to lower the activation threshold of VR1 highlights VR1′s importance in detecting harmful stimuli, making VR1 a significant target for pain management therapies. Additionally, VR1 may interact with endogenous compounds such as anandamide, further linking VR1 to the cannabinoid signaling pathways in both the nervous and cardiovascular systems.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

VR1 Antibody (E-8) References:

1. Ion channels gated by heat.  |  Cesare, P., et al. 1999. Proc Natl Acad Sci U S A. 96: 7658-63. PMID: 10393876
2. Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide.  |  Zygmunt, PM., et al. 1999. Nature. 400: 452-7. PMID: 10440374
3. Peripheral and central actions of capsaicin and VR1 receptor.  |  Sasamura, T. and Kuraishi, Y. 1999. Jpn J Pharmacol. 80: 275-80. PMID: 10496326
4. Effects of peripheral nerve injury on functional spinal VR1 receptors.  |  Kelly, S. and Chapman, V. 2002. Neuroreport. 13: 1147-50. PMID: 12151758
5. Phosphorylation of vanilloid receptor 1 by Ca2+/calmodulin-dependent kinase II regulates its vanilloid binding.  |  Jung, J., et al. 2004. J Biol Chem. 279: 7048-54. PMID: 14630912
6. Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VR1 using a fluorometric imaging plate reader (FLIPR) assay.  |  Behrendt, HJ., et al. 2004. Br J Pharmacol. 141: 737-45. PMID: 14757700
7. Vanilloid receptor 1 regulates multiple calcium compartments and contributes to Ca2+-induced Ca2+ release in sensory neurons.  |  Kárai, LJ., et al. 2004. J Biol Chem. 279: 16377-87. PMID: 14963041
8. Regulated exocytosis contributes to protein kinase C potentiation of vanilloid receptor activity.  |  Morenilla-Palao, C., et al. 2004. J Biol Chem. 279: 25665-72. PMID: 15066994
9. Purinergic and vanilloid receptor activation releases glutamate from separate cranial afferent terminals in nucleus tractus solitarius.  |  Jin, YH., et al. 2004. J Neurosci. 24: 4709-17. PMID: 15152030
10. Identification and biological evaluation of 4-(3-trifluoromethylpyridin-2-yl)piperazine-1-carboxylic acid (5-trifluoromethylpyridin-2-yl)amide, a high affinity TRPV1 (VR1) vanilloid receptor antagonist.  |  Swanson, DM., et al. 2005. J Med Chem. 48: 1857-72. PMID: 15771431
11. The capsaicin receptor: a heat-activated ion channel in the pain pathway.  |  Caterina, MJ., et al. 1997. Nature. 389: 816-24. PMID: 9349813
12. The cloned capsaicin receptor integrates multiple pain-producing stimuli.  |  Tominaga, M., et al. 1998. Neuron. 21: 531-43. PMID: 9768840

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VR1 Antibody (E-8) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-VR1 Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VR1 Antibody (E-8) Specifications

Alternate Product Name: Trpv1

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-130

Epitope Target: VR1

Epitope Species: rat

Product Reactivity/Detection: VR1

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for VR1 Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin