Anti-VR1 Antibody (E-8) recommended for detection of VR1 of mouse and rat origin by WB, IP, IF and ELISA

Anti-VR1 Antibody (E-8): sc-398417

Anti-VR1 Antibody (E-8) is rated 4.9 out of 5 by 12.
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Datasheets

    • Anti-VR1 Antibody (E-8) is a mouse monoclonal IgG2a κ VR1 antibody, cited in 10 publications, provided at 200 µg/ml
    • raised against amino acids 1-130 mapping at the N-terminus of VR1 of rat origin
    • Anti-VR1 Antibody (E-8) is recommended for detection of VR1 of mouse and rat origin by WB, IP, IF and ELISA
    • Anti-VR1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VR1 (E-8): sc-398417.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for VR1 Antibody (E-8) for WB applications. These reagents are now offered in bundles with VR1 Antibody (E-8) (see ordering information below).
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请问说明书中的阳性对照293 Lysate指的是HeK293细胞的溶解产物吗?

Asked by: WYX111
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

The gel shown for the VR1 Antibody (E-8) sc-398417 is gradient gel or other? Please, provide the composition of the Gel. Thank you

Asked by: Pawas Guru
Thank you for your question. The images for sc-398417 are of a 10% SDS-PAGE polyacrylamide gel.
Answered by: Tech Service
Date published: 2019-10-09

Hello, I was wondering if I could get avail of the free sample of VR1 (E-8): sc-398417 conjugated to AlexaFlour 488 for Flow Cytometry? Many Thanks, Aisling

Asked by: Aisling
Thank you for your question. We do not provide sample sizes for our conjugated primary antibodies. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM).
Answered by: Technical Support
Date published: 2019-01-26

Can this antibody be used with the immunoflourscence of formalin fixed tissues ?? 

Asked by: Sara91
Thank you for your question. VR1 Antibody (E-8): sc-398417 is recommended for detection of VR1 of mouse and rat origin by WB, IP, IF and ELISA. It is not recommended for IHC or IHC(P).
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Could this Ab use in Mouse DRG fluorescent immunostaining?

Asked by: Aurora K
Thank you for your question. This antibody is mouse reactive and should be fine for detection in DRG neurons, as long as VR1 is expressed in your DRG cells. If you have any further questions, please reach out to us at [email protected], or by phone or live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-12-04

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2017-01-27
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Rated 5 out of 5 by from Clear and Specific Results Good antibodies work at WB diluted with 1:200, it's clear that the molecular weight in the right single band is in line with my requirements.
Date published: 2017-11-02
Rated 4 out of 5 by from Great features! Western blots were performed to detect the protein expression of human papillary thyroid cancer BCPAP cells. Cell lysates were extracted by RIPA buffer containing protease inhibitor cocktail. 50 µg of total protein was separated by 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The proteins were electroblotted onto nitrocellulose (NC) membrane. The membranes were probed with the TRPV1 primary antibodies (sc-398417), followed by incubation with horseradish peroxidase conjugated secondary antibody. We got a light band at around 100 kDa, and a dark band at around 50 kDa. So i wonder if this product is able to correspond to human TRPV1.
Date published: 2017-10-22
Rated 5 out of 5 by from Strong singal I have tested this antibody on mice brain lysates . Diluted the antibody at 1:1000 use 5% BSA in TBST.Strong singal at 100kda.
Date published: 2017-08-12
Rated 5 out of 5 by from working very good The antibody working very good, giving clear strong signal in primary mouse brain endothelial cells at indicated dilution 1:500.
Date published: 2017-07-23
Rated 5 out of 5 by from A very good antibody A very good antibody, diluted 1:500 products performed well. I will continue to use this product.
Date published: 2017-06-06
Rated 5 out of 5 by from Antibody effect is very good We use this antibody WB, it is always good, we will continue to use.
Date published: 2017-06-02
Rated 5 out of 5 by from high quality for WB I am so happy that I bought this antibody.I compared it with other supplier`s antibodies,and this antibody is more specific and high quality.
Date published: 2017-05-28
Rated 5 out of 5 by from Will continue to buy antibodies Powerful function of antibody, dilute 1:500 product performance is good. Our company will continue to buy the antibody
Date published: 2017-02-09
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