VPS54 Antibody (B-4): sc-398300
- VPS54 Antibody (B-4) is a mouse monoclonal IgG2a κ VPS54 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 917-944 near the C-terminus of VPS54 of human origin
- VPS54 Antibody (B-4) is recommended for detection of VPS54 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-VPS54 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VPS54 Antibody (B-4) for WB applications. These reagents are now offered in bundles with VPS54 Antibody (B-4) (see ordering information below).
VPS54 Antibody (B-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects VPS54 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VPS54 Antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VPS54, also known as vacuolar protein sorting 54, HCC8, SLP-8p, or VPS54L, is a crucial 977 amino acid protein that localizes to the Golgi apparatus and is a key component of the multi-protein Golgi-associated retrograde protein (GARP) complex. VPS54 plays a vital role in the retrograde transport of early and late endosomes back to the Golgi, which is essential for maintaining cellular homeostasis and proper protein trafficking. The gene encoding VPS54 is located on human chromosome 2, a region that contains over 1,400 genes and represents nearly 8% of the human genome. Mutations in genes located on this chromosome, such as ABCA12, ABCG5, ABCG8, and ALMS1, have been linked to various genetic disorders, highlighting VPS54′s importance in cellular function and potential implications in disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VPS54 Antibody (B-4) References:
- Identification, characterization and cytogenetic mapping of a yeast Vps54 homolog in rat and mouse. | Walter, L., et al. 2002. Gene. 285: 213-20. PMID: 12039048
- Comparative transcription map of the wobbler critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14. | Fuchs, S., et al. 2002. BMC Genet. 3: 14. PMID: 12174196
- Multi-component protein complexes and Golgi membrane trafficking. | Oka, T. and Krieger, M. 2005. J Biochem. 137: 109-14. PMID: 15749823
- Characterization of the human GARP (Golgi associated retrograde protein) complex. | Liewen, H., et al. 2005. Exp Cell Res. 306: 24-34. PMID: 15878329
- Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. | Pérez-Victoria, FJ., et al. 2008. Mol Biol Cell. 19: 2350-62. PMID: 18367545
- Evaluation of the Golgi trafficking protein VPS54 (wobbler) as a candidate for ALS. | Meisler, MH., et al. 2008. Amyotroph Lateral Scler. 9: 141-8. PMID: 18574757
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VPS54 Antibody (B-4) | sc-398300 | 200 µg/ml | $322.00 | |||
VPS54 Antibody (B-4): m-IgG Fc BP-HRP Bundle | sc-530748 | 200 µg Ab; 10 µg BP | $361.00 | |||
VPS54 Antibody (B-4): m-IgGκ BP-HRP Bundle | sc-524283 | 200 µg Ab, 40 µg BP | $361.00 | |||
VPS54 Antibody (B-4): m-IgG2a BP-HRP Bundle | sc-547578 | 200 µg Ab; 10 µg BP | $361.00 | |||
VPS54 Antibody (B-4) AC | sc-398300 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VPS54 Antibody (B-4) HRP | sc-398300 HRP | 200 µg/ml | $322.00 | |||
VPS54 Antibody (B-4) FITC | sc-398300 FITC | 200 µg/ml | $336.00 | |||
VPS54 Antibody (B-4) PE | sc-398300 PE | 200 µg/ml | $349.00 | |||
VPS54 Antibody (B-4) Alexa Fluor® 488 | sc-398300 AF488 | 200 µg/ml | $364.00 | |||
VPS54 Antibody (B-4) Alexa Fluor® 546 | sc-398300 AF546 | 200 µg/ml | $364.00 | |||
VPS54 Antibody (B-4) Alexa Fluor® 594 | sc-398300 AF594 | 200 µg/ml | $364.00 | |||
VPS54 Antibody (B-4) Alexa Fluor® 647 | sc-398300 AF647 | 200 µg/ml | $364.00 | |||
VPS54 Antibody (B-4) Alexa Fluor® 680 | sc-398300 AF680 | 200 µg/ml | $364.00 | |||
VPS54 Antibody (B-4) Alexa Fluor® 790 | sc-398300 AF790 | 200 µg/ml | $364.00 | |||
VPS54 (B-4) Neutralizing Peptide | sc-398300 P | 100 µg/0.5 ml | $69.00 |