VPS53 Antibody (H-4): sc-514920
- VPS53 Antibody (H-4) is a mouse monoclonal IgG1 κ VPS53 antibody provided at 200 µg/ml
- raised against amino acids 170-343 mapping within an internal region of VPS53 of human origin
- VPS53 Antibody (H-4) is recommended for detection of VPS53 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-VPS53 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for VPS53 Antibody (H-4) for WB applications. These reagents are now offered in bundles with VPS53 Antibody (H-4) (see ordering information below).
VPS53 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects VPS53 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VPS53 Antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VPS53, also known as vacuolar protein sorting 53 or PP13624, is a crucial 699 amino acid protein that plays a significant role in protein trafficking within the cell. VPS53 is primarily located in the Golgi apparatus and endosome membrane, where VPS53 is essential for retrograde transport of early and late endosomes back to the Golgi. This transport maintains cellular homeostasis and ensures proteins are correctly sorted and delivered to their functional destinations. VPS53 is a key component of the multi-protein Golgi-associated retrograde protein (GARP) complex, which is involved in recycling proteins and lipids, thereby influencing various cellular processes, including secretion and membrane dynamics. The gene encoding VPS53 is located on human chromosome 17, a region encompassing over 2.5% of the human genome and containing more than 1,200 genes, highlighting VPS53′s importance in cellular function and genetic regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VPS53 Antibody (H-4) References:
- The minimum LOH region defined on chromosome 17p13.3 in human hepatocellular carcinoma with gene content analysis. | Zhao, X., et al. 2003. Cancer Lett. 190: 221-32. PMID: 12565177
- Multi-component protein complexes and Golgi membrane trafficking. | Oka, T. and Krieger, M. 2005. J Biochem. 137: 109-14. PMID: 15749823
- Characterization of the human GARP (Golgi associated retrograde protein) complex. | Liewen, H., et al. 2005. Exp Cell Res. 306: 24-34. PMID: 15878329
- Transcription of the putative tumor suppressor gene HCCS1 requires binding of ETS-2 to its consensus near the transcription start site. | Zhu, JD., et al. 2006. Cell Res. 16: 780-96. PMID: 16953216
- Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways. | Morishita, M., et al. 2007. Traffic. 8: 1231-45. PMID: 17645731
- The tail-anchoring domain of Bfl1 and HCCS1 targets mitochondrial membrane permeability to induce apoptosis. | Ko, JK., et al. 2007. J Cell Sci. 120: 2912-23. PMID: 17666431
- Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. | Pérez-Victoria, FJ., et al. 2008. Mol Biol Cell. 19: 2350-62. PMID: 18367545
- VPS53 mutations cause progressive cerebello-cerebral atrophy type 2 (PCCA2). | Feinstein, M., et al. 2014. J Med Genet. 51: 303-8. PMID: 24577744
- GZFLW Induces Apoptosis of Ectopic Endometrial Stromal Cells via Promoting VPS53 Protein Stability. | Zhang, Z., et al. 2018. Evid Based Complement Alternat Med. 2018: 1293630. PMID: 30643524
- VPS53 gene is associated with a new phenotype of complicated hereditary spastic paraparesis. | Hausman-Kedem, M., et al. 2019. Neurogenetics. 20: 187-195. PMID: 31418091
- VPS53 Suppresses Malignant Properties in Colorectal Cancer by Inducing the Autophagy Signaling Pathway. | Peng, H., et al. 2020. Onco Targets Ther. 13: 10667-10675. PMID: 33116643
- Hepatic manifestations in VPS53-related pontocerebellar hypoplasia type 2E: A case report. | Mouchez, A., et al. 2025. Eur J Med Genet. 74: 104996. PMID: 39842660
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VPS53 Antibody (H-4) | sc-514920 | 200 µg/ml | $316.00 | |||
VPS53 Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-527958 | 200 µg Ab; 10 µg BP | $354.00 | |||
VPS53 Antibody (H-4): m-IgG1 BP-HRP Bundle | sc-533331 | 200 µg Ab; 20 µg BP | $354.00 | |||
VPS53 Antibody (H-4) AC | sc-514920 AC | 500 µg/ml, 25% agarose | $416.00 | |||
VPS53 Antibody (H-4) HRP | sc-514920 HRP | 200 µg/ml | $316.00 | |||
VPS53 Antibody (H-4) FITC | sc-514920 FITC | 200 µg/ml | $330.00 | |||
VPS53 Antibody (H-4) PE | sc-514920 PE | 200 µg/ml | $343.00 | |||
VPS53 Antibody (H-4) Alexa Fluor® 488 | sc-514920 AF488 | 200 µg/ml | $357.00 | |||
VPS53 Antibody (H-4) Alexa Fluor® 546 | sc-514920 AF546 | 200 µg/ml | $357.00 | |||
VPS53 Antibody (H-4) Alexa Fluor® 594 | sc-514920 AF594 | 200 µg/ml | $357.00 | |||
VPS53 Antibody (H-4) Alexa Fluor® 647 | sc-514920 AF647 | 200 µg/ml | $357.00 | |||
VPS53 Antibody (H-4) Alexa Fluor® 680 | sc-514920 AF680 | 200 µg/ml | $357.00 | |||
VPS53 Antibody (H-4) Alexa Fluor® 790 | sc-514920 AF790 | 200 µg/ml | $357.00 |