VPS33B Antibody (G-9): sc-398322

VPS33B Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects VPS33B of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VPS33B Antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VPS33B, or vacuolar protein sorting 33 homolog B, is a crucial 617 amino acid protein that plays a significant role in vesicle-mediated protein trafficking, particularly to lysosomal compartments. Localization to the cytoplasmic side of the peripheral membrane and the late endosomal membrane is vital for proper functioning of cellular processes, as VPS33B facilitates the docking of vesicles and sorting of proteins destined for degradation or recycling. VPS33B expression is ubiquitous, with highest levels found in the testis and lowest in the lung, indicating an essential role in various physiological processes. Defects in VPS33B are linked to arthrogryposis-renal dysfunction-cholestasis syndrome (ARC), an autosomal recessive disorder characterized by renal tubular dysfunction, neurogenic arthrogryposis multiplex congenita, and neonatal cholestasis with bile duct hypoplasia, underscoring VPS33B′s critical function in human health.

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VPS33B Antibody (G-9) References:

1. Cloning, mapping and expression analysis of VPS33B, the human orthologue of rat Vps33b.  |  Carim, L., et al. 2000. Cytogenet Cell Genet. 89: 92-5. PMID: 10894945
2. Molecular cloning and characterization of human VPS18, VPS 11, VPS16, and VPS33.  |  Huizing, M., et al. 2001. Gene. 264: 241-7. PMID: 11250079
3. Mutations in VPS33B, encoding a regulator of SNARE-dependent membrane fusion, cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome.  |  Gissen, P., et al. 2004. Nat Genet. 36: 400-4. PMID: 15052268
4. Comparative evolutionary analysis of VPS33 homologues: genetic and functional insights.  |  Gissen, P., et al. 2005. Hum Mol Genet. 14: 1261-70. PMID: 15790593
5. Requirement of VPS33B, a member of the Sec1/Munc18 protein family, in megakaryocyte and platelet alpha-granule biogenesis.  |  Lo, B., et al. 2005. Blood. 106: 4159-66. PMID: 16123220
6. Defective lamellar granule secretion in arthrogryposis, renal dysfunction, and cholestasis syndrome caused by a mutation in VPS33B.  |  Hershkovitz, D., et al. 2008. Arch Dermatol. 144: 334-40. PMID: 18347289
7. Mycobacterium tuberculosis virulence is mediated by PtpA dephosphorylation of human vacuolar protein sorting 33B.  |  Bach, H., et al. 2008. Cell Host Microbe. 3: 316-22. PMID: 18474358
8. Clinical characteristics and VPS33B mutations in patients with ARC syndrome.  |  Jang, JY., et al. 2009. J Pediatr Gastroenterol Nutr. 48: 348-54. PMID: 19274792
9. VPS33B suppresses lung adenocarcinoma metastasis and chemoresistance to cisplatin.  |  Liu, Z., et al. 2021. Genes Dis. 8: 307-319. PMID: 33997178
10. Vps33B in Dendritic Cells Regulates House Dust Mite-Induced Allergic Lung Inflammation.  |  Ma, J., et al. 2021. J Immunol. 207: 2649-2659. PMID: 34732466
11. Vps33B controls Treg cell suppressive function through inhibiting lysosomal nutrient sensing complex-mediated mTORC1 activation.  |  Xiang, H., et al. 2022. Cell Rep. 39: 110943. PMID: 35705052
12. The Sec1-Munc18 protein VPS33B forms a uniquely bidirectional complex with VPS16B.  |  Liu, RJY., et al. 2023. J Biol Chem. 299: 104718. PMID: 37062417

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VPS33B Antibody (G-9) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-VPS33B Antibody (G-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VPS33B Antibody (G-9) Specifications

Alternate Product Name: VPS33B

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 271-570

Epitope Target: VPS33B

Epitope Species: human

Product Reactivity/Detection: VPS33B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for VPS33B Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin