VPS29 Antibody (D-1): sc-398874
- VPS29 Antibody (D-1) is a mouse monoclonal IgG2a κ VPS29 antibody, cited in 11 publications, provided at 200 µg/ml
- raised against amino acids 1-179 mapping at the N-terminus of VPS29 of human origin
- VPS29 Antibody (D-1) is recommended for detection of VPS29 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-VPS29 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VPS29 Antibody (D-1) for WB applications. These reagents are now offered in bundles with VPS29 Antibody (D-1) (see ordering information below).
VPS29 Antibody (D-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects VPS29 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VPS29 Antibody (D-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VPS29, also known as vacuolar protein sorting 29 homolog, DC7, or PEP11, is a crucial component of the retromer complex, which is essential for retrograde transport of proteins from endosomes to trans-Golgi network. This 182 amino acid peripheral membrane protein is predominantly localized in cytoplasm and endosomal membranes, playing a vital role in trafficking of endocytic and biosynthetic proteins to vacuole. Proper functioning of VPS29 is critical for maintaining cellular homeostasis and ensuring proteins are correctly sorted and delivered to intended destinations. VPS29 interacts with other proteins within retromer complex, such as VPS26 and VPS35, which together facilitate recognition and sorting of cargo proteins, underscoring VPS29′s importance in cellular transport mechanisms. Multiple isoforms of VPS29 arise from alternative splicing, contributing to complexity and regulation of function across different tissues, including high expression levels in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon, skeletal muscle, kidney, and brain.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VPS29 Antibody (D-1) References:
- The human Vps29 retromer component is a metallo-phosphoesterase for a cation-independent mannose 6-phosphate receptor substrate peptide. | Damen, E., et al. 2006. Biochem J. 398: 399-409. PMID: 16737443
- AtVPS29, a putative component of a retromer complex, is required for the efficient sorting of seed storage proteins. | Shimada, T., et al. 2006. Plant Cell Physiol. 47: 1187-94. PMID: 16926167
- Interchangeable but essential functions of SNX1 and SNX2 in the association of retromer with endosomes and the trafficking of mannose 6-phosphate receptors. | Rojas, R., et al. 2007. Mol Cell Biol. 27: 1112-24. PMID: 17101778
- A loss-of-function screen reveals SNX5 and SNX6 as potential components of the mammalian retromer. | Wassmer, T., et al. 2007. J Cell Sci. 120: 45-54. PMID: 17148574
- Phosphoinositide 3-kinase regulates the role of retromer in transcytosis of the polymeric immunoglobulin receptor. | Vergés, M., et al. 2007. Exp Cell Res. 313: 707-18. PMID: 17184770
- Arabidopsis vacuolar sorting mutants (green fluorescent seed) can be identified efficiently by secretion of vacuole-targeted green fluorescent protein in their seeds. | Fuji, K., et al. 2007. Plant Cell. 19: 597-609. PMID: 17293568
- Functional architecture of the retromer cargo-recognition complex. | Hierro, A., et al. 2007. Nature. 449: 1063-7. PMID: 17891154
- AP-1 and retromer play opposite roles in the trafficking of sortilin between the Golgi apparatus and the lysosomes. | Canuel, M., et al. 2008. Biochem Biophys Res Commun. 366: 724-30. PMID: 18078806
- Identification of novel retromer complexes in the mouse testis. | Kim, E., et al. 2008. Biochem Biophys Res Commun. 375: 16-21. PMID: 18656452
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VPS29 Antibody (D-1) | sc-398874 | 200 µg/ml | $322.00 | |||
VPS29 Antibody (D-1): m-IgG Fc BP-HRP Bundle | sc-530950 | 200 µg Ab; 10 µg BP | $361.00 | |||
VPS29 Antibody (D-1): m-IgGκ BP-HRP Bundle | sc-524516 | 200 µg Ab, 40 µg BP | $361.00 | |||
VPS29 Antibody (D-1): m-IgG2a BP-HRP Bundle | sc-547643 | 200 µg Ab; 10 µg BP | $361.00 | |||
VPS29 Antibody (D-1) AC | sc-398874 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VPS29 Antibody (D-1) HRP | sc-398874 HRP | 200 µg/ml | $322.00 | |||
VPS29 Antibody (D-1) FITC | sc-398874 FITC | 200 µg/ml | $336.00 | |||
VPS29 Antibody (D-1) PE | sc-398874 PE | 200 µg/ml | $349.00 | |||
VPS29 Antibody (D-1) Alexa Fluor® 488 | sc-398874 AF488 | 200 µg/ml | $364.00 | |||
VPS29 Antibody (D-1) Alexa Fluor® 546 | sc-398874 AF546 | 200 µg/ml | $364.00 | |||
VPS29 Antibody (D-1) Alexa Fluor® 594 | sc-398874 AF594 | 200 µg/ml | $364.00 | |||
VPS29 Antibody (D-1) Alexa Fluor® 647 | sc-398874 AF647 | 200 µg/ml | $364.00 | |||
VPS29 Antibody (D-1) Alexa Fluor® 680 | sc-398874 AF680 | 200 µg/ml | $364.00 | |||
VPS29 Antibody (D-1) Alexa Fluor® 790 | sc-398874 AF790 | 200 µg/ml | $364.00 |