VP16 Antibody (14-5): sc-7546

VP16 Antibody (14-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects VP16 in various applications including western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-VP16 antibody (14-5) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for different experimental needs. VP16, also known as the Herpes simplex virus protein vmw65, plays a crucial role in the viral life cycle by acting as a transcriptional activator, which is essential for the expression of immediate-early genes necessary for viral replication. This function is particularly important as VP16 allows the virus to hijack the host′s cellular machinery, facilitating efficient viral propagation and pathogenesis. Understanding the interactions and mechanisms of VP16 can provide insights into potential therapeutic targets for herpesvirus infections. The two-hybrid system, a widely-used method for detecting protein-protein interactions, can be employed to study VP16′s interactions with other proteins, enhancing our understanding of VP16′s role in the viral lifecycle and impact on host cell functions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

VP16 Antibody (14-5) References:

1. VP16-dependent association of chromatin-modifying coactivators and underrepresentation of histones at immediate-early gene promoters during herpes simplex virus infection.  |  Herrera, FJ. and Triezenberg, SJ. 2004. J Virol. 78: 9689-96. PMID: 15331701
2. Gal4-VP16 directs ATP-independent chromatin reorganization in a yeast chromatin assembly system.  |  Robinson, KM. and Schultz, MC. 2005. Biochemistry. 44: 4551-61. PMID: 15766286
3. The VP16 activation domain establishes an active mediator lacking CDK8 in vivo.  |  Uhlmann, T., et al. 2007. J Biol Chem. 282: 2163-73. PMID: 17135252
4. Structure of the VP16 transactivator target in the Mediator.  |  Milbradt, AG., et al. 2011. Nat Struct Mol Biol. 18: 410-5. PMID: 21378963
5. VP16 serine 375 is a critical determinant of herpes simplex virus exit from latency in vivo.  |  Sawtell, NM., et al. 2011. J Neurovirol. 17: 546-51. PMID: 22144074
6. GAL4-VP16 is an unusually potent transcriptional activator.  |  Sadowski, I., et al. 1988. Nature. 335: 563-4. PMID: 3047590
7. Adult patients with Ph+ ALL benefit from conditioning regimen of medium-dose VP16 plus CY/TBI.  |  Morita-Fujita, M., et al. 2022. Hematol Oncol. 40: 1041-1055. PMID: 35790020
8. Slug, a Stress-Induced Transcription Factor, Stimulates Herpes Simplex Virus 1 Replication and Transactivates a cis-Regulatory Module within the VP16 Promoter.  |  Santos, VC., et al. 2023. J Virol. 97: e0007323. PMID: 37022165
9. Neuronal expression of herpes simplex virus-1 VP16 protein induces pseudorabies virus escape from silencing and reactivation.  |  Hsu, Z-S., et al. 2024. J Virol. 98: e0056124. PMID: 38869285
10. Enhancing expression of recombinant proteins in mammalian cells using the herpesvirus VP16 transactivator.  |  Hippenmeyer, PJ. and Pegg, LE. 1995. Curr Opin Biotechnol. 6: 548-52. PMID: 7579666

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VP16 Antibody (14-5) has 29 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VP16 Antibody (14-5) Specifications

Alternate Product Name: Herpes simplex virus protein vmw65

Product Clone Name: 14-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 410-456

Epitope Target: VP16

Epitope Species:

Product Reactivity/Detection: VP16 and VP16 fusion proteins

Species Reactivity/Detection: N/A

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 29 citations in various scientific publications

Available Conjugates/Forms for VP16 Antibody (14-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred VP16 antibody is VP16 Antibody (1-21): sc-7545.