VP16 Antibody (1-21): sc-7545

VP16 Antibody (1-21) is a mouse monoclonal IgG1 kappa light chain antibody that detects VP16 protein by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-VP16 antibody (1-21) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VP16 protein, also known as the 65 kDa tegument protein of herpes simplex virus type 1 (HSV-1), plays a crucial role in viral life cycle by facilitating transcription of immediate-early genes, which are essential for initiating viral replication. VP16 (1-21) monoclonal antibody interacts with host transcription factors, such as Oct-1 and Host Cell Factor 1 (HCF), to enhance transcriptional activity of viral genes, thereby promoting efficient viral replication and pathogenesis. VP16′s ability to modulate host cellular machinery underscores its importance in virology and highlights potential for therapeutic interventions targeting this protein. The two-hybrid system, a widely utilized method for detecting protein-protein interactions, leverages unique properties of VP16, allowing researchers to explore complex protein interactions and their implications in various biological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

VP16 Antibody (1-21) References:

1. GAL4 fusion vectors for expression in yeast or mammalian cells.  |  Sadowski, I., et al. 1992. Gene. 118: 137-41. PMID: 1511877
2. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.  |  Chien, CT., et al. 1991. Proc Natl Acad Sci U S A. 88: 9578-82. PMID: 1946372
3. Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation.  |  Song, D., et al. 1991. Genes Dev. 5: 741-50. PMID: 2026326
4. Activators and targets.  |  Ptashne, M. and Gann, AA. 1990. Nature. 346: 329-31. PMID: 2142753
5. A novel genetic system to detect protein-protein interactions.  |  Fields, S. and Song, O. 1989. Nature. 340: 245-6. PMID: 2547163
6. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae.  |  Johnston, M. 1987. Microbiol Rev. 51: 458-76. PMID: 2830478
7. Deletion analysis of GAL4 defines two transcriptional activating segments.  |  Ma, J. and Ptashne, M. 1987. Cell. 48: 847-53. PMID: 3028647
8. Duck Enteritis Virus VP16 Antagonizes IFN-β-Mediated Antiviral Innate Immunity.  |  Li, Y., et al. 2020. J Immunol Res. 2020: 9630452. PMID: 32537474
9. The Role of VP16 in the Life Cycle of Alphaherpesviruses.  |  Fan, D., et al. 2020. Front Microbiol. 11: 1910. PMID: 33013729
10. Host factors associated with either VP16 or VP16-induced complex differentially affect HSV-1 lytic infection.  |  Ding, X., et al. 2022. Rev Med Virol. 32: e2394. PMID: 36069169
11. VP16-213 and podophyllotoxin. A study on the relationship between chemical structure and biological activity.  |  Loike, JD. 1982. Cancer Chemother Pharmacol. 7: 103-11. PMID: 7044591
12. The two-hybrid system: an assay for protein-protein interactions.  |  Fields, S. and Sternglanz, R. 1994. Trends Genet. 10: 286-92. PMID: 7940758

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VP16 Antibody (1-21) has 112 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VP16 Antibody (1-21) Specifications

Alternate Product Name: Herpes simplex virus protein vmw65

Product Clone Name: 1-21

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 456-490

Epitope Target: VP16

Epitope Species:

Product Reactivity/Detection: VP16 and VP16 fusion proteins

Species Reactivity/Detection: N/A

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 112 citations in various scientific publications

Available Conjugates/Forms for VP16 Antibody (1-21): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine

The preferred VP16 antibody is VP16 Antibody (14-5): sc-7546.