VMAT 1 Antibody (G-12): sc-166391
- VMAT 1 Antibody (G-12) is a mouse monoclonal IgG2b κ VMAT 1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 44-143 mapping near the N-terminus of VMAT 1 of human origin
- VMAT 1 Antibody (G-12) is recommended for detection of VMAT 1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-VMAT 1 Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VMAT 1 Antibody (G-12) for WB applications. These reagents are now offered in bundles with VMAT 1 Antibody (G-12) (see ordering information below).
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VMAT 1 Antibody (G-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects VMAT 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VMAT 1 (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VMAT 1 protein, also known as SLC18A1, plays a crucial role in neurotransmission by facilitating the packaging of monoamines into synaptic vesicles, which is essential for the regulated release of neurotransmitters in neurons and neuroendocrine cells. This process is vital for proper neuronal communication and overall brain function, as it ensures that neurotransmitters are stored and released in a controlled manner, preventing excessive signaling that could lead to neurotoxicity. VMAT 1 structure is characterized by twelve transmembrane domains, which are integral to its function as a vesicular transporter, and shares weak homology with a class of bacterial antibiotic resistance proteins, highlighting its evolutionary significance. The vesicular transport mechanism employed by VMAT 1 is both ATP- and proton-dependent, underscoring the energy requirements for effective neurotransmitter packaging and release.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VMAT 1 Antibody (G-12) References:
- Biochemistry and molecular biology of the vesicular monoamine transporter from chromaffin granules. | Henry, JP., et al. 1994. J Exp Biol. 196: 251-62. PMID: 7823026
- Molecular cloning of a putative vesicular transporter for acetylcholine. | Roghani, A., et al. 1994. Proc Natl Acad Sci U S A. 91: 10620-4. PMID: 7938002
- Acetylcholine active transport by rat brain synaptic vesicles. | Haigh, JR., et al. 1994. Neuroreport. 5: 773-6. PMID: 8018848
- The pharmacological profile of the vesicular monoamine transporter resembles that of multidrug transporters. | Yelin, R. and Schuldiner, S. 1995. FEBS Lett. 377: 201-7. PMID: 8543051
- Active transport of acetylcholine by the human vesicular acetylcholine transporter. | Varoqui, H. and Erickson, JD. 1996. J Biol Chem. 271: 27229-32. PMID: 8910293
- Vesicular neurotransmitter transporters. Potential sites for the regulation of synaptic function. | Varoqui, H. and Erickson, JD. 1997. Mol Neurobiol. 15: 165-91. PMID: 9396009
- Vesicular neurotransmitter transport and the presynaptic regulation of quantal size. | Reimer, RJ., et al. 1998. Curr Opin Neurobiol. 8: 405-12. PMID: 9687352
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VMAT 1 Antibody (G-12) | sc-166391 | 200 µg/ml | $316.00 | |||
VMAT 1 Antibody (G-12): m-IgGκ BP-HRP Bundle | sc-521586 | 200 µg Ab, 40 µg BP | $354.00 | |||
VMAT 1 Antibody (G-12): m-IgG2b BP-HRP Bundle | sc-548933 | 200 µg Ab; 10 µg BP | $354.00 | |||
VMAT 1 Antibody (G-12) AC | sc-166391 AC | 500 µg/ml, 25% agarose | $416.00 | |||
VMAT 1 Antibody (G-12) HRP | sc-166391 HRP | 200 µg/ml | $316.00 | |||
VMAT 1 Antibody (G-12) FITC | sc-166391 FITC | 200 µg/ml | $330.00 | |||
VMAT 1 Antibody (G-12) PE | sc-166391 PE | 200 µg/ml | $343.00 | |||
VMAT 1 Antibody (G-12) Alexa Fluor® 488 | sc-166391 AF488 | 200 µg/ml | $357.00 | |||
VMAT 1 Antibody (G-12) Alexa Fluor® 546 | sc-166391 AF546 | 200 µg/ml | $357.00 | |||
VMAT 1 Antibody (G-12) Alexa Fluor® 594 | sc-166391 AF594 | 200 µg/ml | $357.00 | |||
VMAT 1 Antibody (G-12) Alexa Fluor® 647 | sc-166391 AF647 | 200 µg/ml | $357.00 | |||
VMAT 1 Antibody (G-12) Alexa Fluor® 680 | sc-166391 AF680 | 200 µg/ml | $357.00 | |||
VMAT 1 Antibody (G-12) Alexa Fluor® 790 | sc-166391 AF790 | 200 µg/ml | $357.00 |