Vinexin Antibody (D-4): sc-398275

Vinexin Antibody (D-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Vinexin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Vinexin antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Vinexin, also known as SORBS3 or SCAM1, is a crucial cytoskeletal protein that exists in three isoforms: Vinexin α, β, and γ. These isoforms are essential for cell adhesion, cytoskeletal organization, and cell signaling, particularly in promoting actin stress fiber formation and activating c-Jun N-terminal kinase (JNK) pathway in response to epidermal growth factor (EGF) stimulation. Vinexin′s structure features three SH3 domains and one sorbin homology (SoHo) domain, which facilitate interactions with other proteins; specifically, binding to vinculin through first two SH3 domains and to guanine nucleotide exchange factor SOS via third SH3 domain. Vinexin expression occurs in various tissues, including placenta, heart, liver, brain, pancreas, and skeletal muscle, with localization at focal adhesion sites, cell-cell junctions, and cell-extracellular matrix junctions, while β isoform specifically resides in the nucleus, highlighting diverse functional roles within cells.

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Vinexin Antibody (D-4) References:

1. Vinexin forms a signaling complex with Sos and modulates epidermal growth factor-induced c-Jun N-terminal kinase/stress-activated protein kinase activities.  |  Akamatsu, M., et al. 1999. J Biol Chem. 274: 35933-7. PMID: 10585480
2. Vinexin, CAP/ponsin, ArgBP2: a novel adaptor protein family regulating cytoskeletal organization and signal transduction.  |  Kioka, N., et al. 2002. Cell Struct Funct. 27: 1-7. PMID: 11937713
3. The focal adhesion protein vinexin alpha regulates the phosphorylation and activity of estrogen receptor alpha.  |  Tujague, M., et al. 2004. J Biol Chem. 279: 9255-63. PMID: 14625289
4. A novel isoform of Vinexin, Vinexin gamma, regulates Sox9 gene expression through activation of MAPK cascade in mouse fetal gonad.  |  Matsuyama, M., et al. 2005. Genes Cells. 10: 421-34. PMID: 15836771
5. SHIP2 interaction with the cytoskeletal protein Vinexin.  |  Paternotte, N., et al. 2005. FEBS J. 272: 6052-66. PMID: 16302969
6. Vinexin beta regulates the phosphorylation of epidermal growth factor receptor on the cell surface.  |  Mitsushima, M., et al. 2006. Genes Cells. 11: 971-82. PMID: 16923119
7. Phosphorylation by extracellular signal-regulated kinase of a multidomain adaptor protein, vinexin, at synapses.  |  Ito, H., et al. 2007. J Neurochem. 100: 545-54. PMID: 17241162
8. Vinexin-β protects against cardiac hypertrophy by blocking the Akt-dependent signalling pathway.  |  Chen, K., et al. 2013. Basic Res Cardiol. 108: 338. PMID: 23429936
9. Vinexin: a novel vinculin-binding protein with multiple SH3 domains enhances actin cytoskeletal organization.  |  Kioka, N., et al. 1999. J Cell Biol. 144: 59-69. PMID: 9885244

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Vinexin Antibody (D-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Vinexin Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Vinexin Antibody (D-4) Specifications

Alternate Product Name: Vinexin

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 206-243

Epitope Target: Vinexin

Epitope Species: human

Product Reactivity/Detection: Vinexin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Vinexin Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin