vinculin Antibody (7F9): sc-73614
- vinculin Antibody (7F9) is a mouse monoclonal IgG1 κ vinculin antibody, cited in 476 publications, provided at 200 µg/ml
- raised against vinculin of human origin
- vinculin Antibody (7F9) is recommended for detection of vinculin of human, mouse, rat and avian origin by WB, IP, IF and IHC(P)
- Anti-vinculin Antibody (7F9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for vinculin Antibody (7F9) for WB and IHC(P) applications. These reagents are now offered in bundles with vinculin Antibody (7F9) (see ordering information below).
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Vinculin Antibody (7F9) is a mouse monoclonal IgG1 kappa light chain antibody that detects vinculin protein of human, mouse, rat, and avian origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-vinculin antibody (7F9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Vinculin plays a crucial role in cellular adhesion and mechanotransduction, functioning as a key component of focal adhesions, which are specialized structures that connect the cytoskeleton to the extracellular matrix. These focal adhesions are essential for maintaining cell shape, enabling cell migration, and facilitating communication between the cell and its environment. Vinculin′s ability to interact with other cytoskeletal proteins, such as Talin and α-actinin, underscores vinculin′s importance in linking the actin cytoskeleton to integrins in the plasma membrane, thereby influencing cellular responses to mechanical stimuli. Furthermore, vinculin and Talin expression levels are closely tied to the dynamics of actin filaments, highlighting the intricate regulatory mechanisms that govern cell adhesion and movement. With versatile applications and critical role in cell biology, vinculin monoclonal antibody (7F9) serves as an invaluable tool for researchers studying cellular adhesion and signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
vinculin Antibody (7F9) References:
- Further characterisation of the talin-binding site in the cytoskeletal protein vinculin. | Gilmore, AP., et al. 1992. J Cell Sci. 103 (Pt 3): 719-31. PMID: 1478968
- Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. | Burridge, K., et al. 1988. Annu Rev Cell Biol. 4: 487-525. PMID: 3058164
- Impact of altered actin gene expression on vinculin, talin, cell spreading, and motility. | Schevzov, G., et al. 1995. DNA Cell Biol. 14: 689-700. PMID: 7646816
- Modulation of alpha-actinin levels affects cell motility and confers tumorigenicity on 3T3 cells. | Glück, U. and Ben-Ze'ev, A. 1994. J Cell Sci. 107 (Pt 7): 1773-82. PMID: 7983147
- Characterisation of the paxillin-binding site and the C-terminal focal adhesion targeting sequence in vinculin. | Wood, CK., et al. 1994. J Cell Sci. 107 (Pt 2): 709-17. PMID: 8207093
- Regulation of vinculin binding to talin and actin by phosphatidyl-inositol-4-5-bisphosphate. | Gilmore, AP. and Burridge, K. 1996. Nature. 381: 531-5. PMID: 8632828
- Talin contains three actin-binding sites each of which is adjacent to a vinculin-binding site. | Hemmings, L., et al. 1996. J Cell Sci. 109 (Pt 11): 2715-26. PMID: 8937989
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
vinculin Antibody (7F9) | sc-73614 | 200 µg/ml | $322.00 | |||
vinculin Antibody (7F9): m-IgG Fc BP-HRP Bundle | sc-528657 | 200 µg Ab; 10 µg BP | $361.00 | |||
vinculin Antibody (7F9): m-IgGκ BP-HRP Bundle | sc-521102 | 200 µg Ab, 40 µg BP | $361.00 | |||
vinculin Antibody (7F9): m-IgG1 BP-HRP Bundle | sc-543056 | 200 µg Ab; 20 µg BP | $361.00 | |||
vinculin Antibody (7F9) AC | sc-73614 AC | 500 µg/ml, 25% agarose | $424.00 | |||
vinculin Antibody (7F9) HRP | sc-73614 HRP | 200 µg/ml | $322.00 | |||
vinculin Antibody (7F9) FITC | sc-73614 FITC | 200 µg/ml | $336.00 | |||
vinculin Antibody (7F9) PE | sc-73614 PE | 200 µg/ml | $349.00 | |||
vinculin Antibody (7F9) Alexa Fluor® 488 | sc-73614 AF488 | 200 µg/ml | $364.00 | |||
vinculin Antibody (7F9) Alexa Fluor® 546 | sc-73614 AF546 | 200 µg/ml | $364.00 | |||
vinculin Antibody (7F9) Alexa Fluor® 594 | sc-73614 AF594 | 200 µg/ml | $364.00 | |||
vinculin Antibody (7F9) Alexa Fluor® 647 | sc-73614 AF647 | 200 µg/ml | $364.00 | |||
vinculin Antibody (7F9) Alexa Fluor® 680 | sc-73614 AF680 | 200 µg/ml | $364.00 | |||
vinculin Antibody (7F9) Alexa Fluor® 790 | sc-73614 AF790 | 200 µg/ml | $364.00 |