Vimentin Antibody (9E7E7): sc-66001

Vimentin Antibody (9E7E7) is a mouse monoclonal IgG1 kappa light chain antibody raised against truncated recombinant human vimentin, a fundamental component of cytoskeletal intermediate filaments. Vimentin mouse monoclonal antibody (9E7E7) recognizes an epitope within the vimentin immunogen sequence and demonstrates effectiveness in Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry on paraffin-embedded sections. Anti-vimentin monoclonal antibody (9E7E7) serves as a valuable marker for identifying mesenchymal cells and distinguishing tumor origins, making vimentin particularly useful in cancer and metastasis research. Vimentin forms part of the intermediate filament network that provides structural integrity to cells while participating in essential cellular functions such as migration, wound healing, and epithelial-mesenchymal transition (EMT). In many cancers, including melanoma and breast carcinoma, vimentin shows co-expression with other intermediate filament proteins like cytokeratins. Vimentin engages in several crucial protein interactions that extend beyond structural roles. For example, vimentin′s association with integrins facilitates cell migration processes, while vimentin′s interaction with plectin helps stabilize cellular architecture by connecting vimentin filaments to other cytoskeletal components. The relationship between vimentin and AKT kinase adds another layer of regulation, as AKT-mediated phosphorylation of vimentin influences cell survival and motility mechanisms - processes fundamental to cancer progression and metastasis. Through these various interactions and modifications, vimentin coordinates cellular structure with signaling pathways that control cell behavior and fate. Vimentin (9E7E7) antibody enables researchers to investigate these complex cellular processes in both normal and pathological conditions.

The preferred Vimentin antibody is Vimentin Antibody (V9): sc-6260.