Vimentin Antibody (9E7E7) recommended for detection of Vimentin of human origin by WB, IP, IF and IHC(P)

Vimentin Antibody (9E7E7): sc-66001

Vimentin Antibody (9E7E7) is rated 5.0 out of 5 by 3.
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Datasheets

    • Vimentin Antibody (9E7E7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against purified truncated recombinant Vimentin of human origin
    • recommended for detection of Vimentin of human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using Vimentin (9E7E7): sc-66001 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic and membrane Great immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
Date published: 2015-02-11
Rated 5 out of 5 by from Robust bands in WB in SJRH30 and RD whole Robust bands in WB in SJRH30 and RD whole cell lysates. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from Publishable data for use in IHC (PFA) in Publishable data for use in IHC (PFA) in human oral cavity tumor sample. -SCBT Publication Review
Date published: 2015-01-27
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